scholarly journals Pressure and Curvature Control of Contact Inhibition in Epithelia Growing Under Spherical Confinement

2021 ◽  
Author(s):  
Ilaria Di Meglio ◽  
Anastasiya Trushko ◽  
Pau Guillamat ◽  
Carles Blanch-Mercader ◽  
Aurélien Roux
Keyword(s):  
Contact Inhibition ◽  
Curvature Control

scholarly journals Pressure and curvature control of contact inhibition in epithelia growing under spherical confinement

2021 ◽  
Author(s):  
Ilaria Di Meglio ◽  
Anastasiya Trushko ◽  
Pau Guillamat ◽  
Carles Blanch-Mercader ◽  
Aurelien Roux
Keyword(s):  
Cell Proliferation ◽  
Threshold Value ◽  
Tissue Level ◽  
Contact Inhibition ◽  
Local Curvature ◽  
Inhibition Of Proliferation ◽  
Spatiotemporal Regulation ◽  
Pathway Inhibition ◽  
Pressure Changes ◽  
Curvature Control

Morphogenesis requires spatiotemporal regulation of cell shape and proliferation, both regulated by biochemical and mechanical cues. In epithelia, this regulation is called contact inhibition, but disentangling biochemical from mechanical cues remains challenging. Here, we show that epithelia growing under confinement accumulate pressure that inhibits proliferation above a threshold value, which depends on the β-catenin pathway. Before inhibition of proliferation, cell aspect ratio abruptly increased upon reaching confluency. This shape transition occurred at low, constant pressure and was mainly controlled by cell density and contractility, correlating with YAP/TAZ pathway inhibition. In our system, epithelia spontaneously buckle: we observed that folding transiently reactivates both the YAP/TAZ pathway and cell proliferation. Altogether, our results support that different mechanical cues part of contact inhibition regulate cell proliferation through different mechanosensing pathways. Proliferation is regulated by sustained, tissue-level pressure through the β-catenin pathway, and by local curvature and pressure changes through the YAP/TAZ pathway.

scholarly journals Modelling cell guidance and curvature control in evolving biological tissues

2021 ◽  
pp. 110658
Author(s):  
Solene G.D. Hegarty-Cremer ◽  
Matthew J. Simpson ◽  
Thomas L. Andersen ◽  
Pascal R. Buenzli
Keyword(s):  
Biological Tissues ◽  
Cell Guidance ◽  
Curvature Control
Sign in / Sign up

Export Citation Format

Share Document