Long-Term Selective Stimulation of Transplanted Neural Stem/Progenitor Cells for Spinal Cord Injury Improves Locomotor Function

2021 ◽  
Author(s):  
Momotaro Kawai ◽  
Kent Imaizumi ◽  
Mitsuru Ishikawa ◽  
Shinsuke Shibata ◽  
Munehisa Shinozaki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Locomotor Function ◽  
Selective Stimulation ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells ◽  
Stimulation Of

scholarly journals Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor function

Cell Reports ◽  
2021 ◽  
Vol 37 (8) ◽  
pp. 110019
Author(s):  
Momotaro Kawai ◽  
Kent Imaizumi ◽  
Mitsuru Ishikawa ◽  
Shinsuke Shibata ◽  
Munehisa Shinozaki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Locomotor Function ◽  
Selective Stimulation ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells ◽  
Stimulation Of

Transplantation of Neural Stem/Progenitor Cells at Different Locations in Mice with Spinal Cord Injury

2014 ◽  
Vol 23 (11) ◽  
pp. 1451-1464 ◽  
Author(s):  
Hiroki Iwai ◽  
Satoshi Nori ◽  
Soraya Nishimura ◽  
Akimasa Yasuda ◽  
Morito Takano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Functional Recovery ◽  
Control Group ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells ◽  
Neurotropic Factor ◽  
Transplantation Site

Transplantation of neural stem/progenitor cells (NS/PCs) promotes functional recovery after spinal cord injury (SCI); however, few studies have examined the optimal site of NS/PC transplantation in the spinal cord. The purpose of this study was to determine the optimal transplantation site of NS/PCs for the treatment of SCI. Wild-type mice were generated with contusive SCI at the T10 level, and NS/PCs were derived from fetal transgenic mice. These NS/PCs ubiquitously expressed ffLuc-cp156 protein (Venus and luciferase fusion protein) and so could be detected by in vivo bioluminescence imaging 9 days postinjury. NS/PCs (low: 250,000 cells per mouse; high: 1 million cells per mouse) were grafted into the spinal cord at the lesion epicenter (E) or at rostral and caudal (RC) sites. Phosphate-buffered saline was injected into E as a control. Motor functional recovery was better in each of the transplantation groups (E-Low, E-High, RC-Low, and RC-High) than in the control group. The photon counts of the grafted NS/PCs were similar in each of the four transplantation groups, suggesting that the survival of NS/PCs was fairly uniform when more than a certain threshold number of cells were transplanted. Quantitative RT-PCR analyses demonstrated that brain-derived neurotropic factor expression was higher in the RC segment than in the E segment, and this may underlie why NS/PCs more readily differentiated into neurons than into astrocytes in the RC group. The location of the transplantation site did not affect the area of spared fibers, angiogenesis, or the expression of any other mediators. These findings indicated that the microenvironments of the E and RC sites are able to support NS/PCs transplanted during the subacute phase of SCI similarly. Optimally, a certain threshold number of NS/PCs should be grafted into the E segment to avoid damaging sites adjacent to the lesion during the injection procedure.

scholarly journals Comparative Study of Methods for Administering Neural Stem/Progenitor Cells to Treat Spinal Cord Injury in Mice

2011 ◽  
Vol 20 (5) ◽  
pp. 727-739 ◽  
Author(s):  
Yuichiro Takahashi ◽  
Osahiko Tsuji ◽  
Gentaro Kumagai ◽  
Chikako Miyauchi Hara ◽  
Hirotaka James Okano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Comparative Study ◽  
Progenitor Cells ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells

scholarly journals Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold

2021 ◽  
Author(s):  
Sara Abdolahi ◽  
Hadi Aligholi ◽  
Azizollah Khodakaram-Tafti ◽  
Maryam Khaleghi Ghadiri ◽  
Walter Stummer ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Neural Circuit ◽  
Lentiviral Vector ◽  
Host Tissue ◽  
Lesion Volume ◽  
Cell Based Therapy ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells

AbstractSpinal cord injury (SCI) is a disabling neurological disorder that causes neural circuit dysfunction. Although various therapies have been applied to improve the neurological outcomes of SCI, little clinical progress has been achieved. Stem cell–based therapy aimed at restoring the lost cells and supporting micromilieu at the site of the injury has become a conceptually attractive option for tissue repair following SCI. Adult human neural stem/progenitor cells (hNS/PCs) were obtained from the epileptic human brain specimens. Induction of SCI was followed by the application of lentiviral vector-mediated green fluorescent protein–labeled hNS/PCs seeded in PuraMatrix peptide hydrogel (PM). The co-application of hNS/PCs and PM at the SCI injury site significantly enhanced cell survival and differentiation, reduced the lesion volume, and improved neurological functions compared to the control groups. Besides, the transplanted hNS/PCs seeded in PM revealed significantly higher migration abilities into the lesion site and the healthy host tissue as well as a greater differentiation into astrocytes and neurons in the vicinity of the lesion as well as in the host tissue. Our data suggest that the transplantation of hNS/PCs seeded in PM could be a promising approach to restore the damaged tissues and improve neurological functions after SCI.

scholarly journals Cell Therapy for Spinal Cord Injury by Neural Stem/Progenitor Cells Derived from iPS/ES Cells

2011 ◽  
Vol 8 (4) ◽  
pp. 668-676 ◽  
Author(s):  
Osahiko Tsuji ◽  
Kyoko Miura ◽  
Kanehiro Fujiyoshi ◽  
Suketaka Momoshima ◽  
Masaya Nakamura ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Therapy ◽  
Progenitor Cells ◽  
Es Cells ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells

scholarly journals Comparison between Fetal Spinal-Cord- and Forebrain-Derived Neural Stem/Progenitor Cells as a Source of Transplantation for Spinal Cord Injury

2004 ◽  
Vol 26 (2-4) ◽  
pp. 275-287 ◽  
Author(s):  
Kota Watanabe ◽  
Masaya Nakamura ◽  
Akio Iwanami ◽  
Yuko Fujita ◽  
Yonehiro Kanemura ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells
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