Exosomal Let-7i-5p from Three-Dimensional Cultured Human Umbilical Cord Mesenchymal Stem Cell S Inhibits the Activation of Fibroblasts Induced by Silica Through Targeting TGFBR1

2021 ◽  
Author(s):  
Chunjie Xu ◽  
Lin Hou ◽  
Jing Zhao ◽  
Zhonghui Zhu ◽  
Fuyang Jiang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Three Dimensional ◽  
Human Umbilical Cord

scholarly journals Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial–mesenchymal transition by targeting HOXC6

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dongli Li ◽  
Junxiu Zhang ◽  
Zijia Liu ◽  
Yuanyuan Gong ◽  
Zhi Zheng
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Mechanism Of Action ◽  
Epithelial Mesenchymal Transition ◽  
Human Umbilical Cord ◽  
Mesenchymal Transition ◽  
Rpe Cells ◽  
Subretinal Fibrosis

Abstract Background and aim Subretinal fibrosis resulting from neovascular age-related macular degeneration (nAMD) is one of the major causes of serious and irreversible vision loss worldwide, and no definite and effective treatment exists currently. Retinal pigmented epithelium (RPE) cells are crucial in maintaining the visual function of normal eyes and its epithelial–mesenchymal transition (EMT) is associated with the pathogenesis of subretinal fibrosis. Stem cell-derived exosomes have been reported to play a crucial role in tissue fibrosis by transferring their molecular contents. This study aimed to explore the effects of human umbilical cord-derived mesenchymal stem cell exosomes (hucMSC-Exo) on subretinal fibrosis in vivo and in vitro and to investigate the anti-fibrotic mechanism of action of hucMSC-Exo. Methods In this study, human umbilical cord-derived mesenchymal stem cells (hucMSCs) were successfully cultured and identified, and exosomes were isolated from the supernatant by ultracentrifugation. A laser-induced choroidal neovascularization (CNV) and subretinal fibrosis model indicated that the intravitreal administration of hucMSC-Exo effectively alleviated subretinal fibrosis in vivo. Furthermore, hucMSC-Exo could efficaciously suppress the migration of retinal pigmented epithelial (RPE) cells and promote the mesenchymal–epithelial transition by delivering miR-27b-3p. The latent binding of miR-27b-3p to homeobox protein Hox-C6 (HOXC6) was analyzed by bioinformatics prediction and luciferase reporter assays. Results This study showed that the intravitreal injection of hucMSC-Exo effectively ameliorated laser-induced CNV and subretinal fibrosis via the suppression of epithelial–mesenchymal transition (EMT) process. In addition, hucMSC-Exo containing miR-27b repressed the EMT process in RPE cells induced by transforming growth factor-beta2 (TGF-β2) via inhibiting HOXC6 expression. Conclusions The present study showed that HucMSC-derived exosomal miR-27b could reverse the process of EMT induced by TGF-β2 via inhibiting HOXC6, indicating that the exosomal miR-27b/HOXC6 axis might play a vital role in ameliorating subretinal fibrosis. The present study proposed a promising therapeutic agent for treating ocular fibrotic diseases and provided insights into the mechanism of action of hucMSC-Exo on subretinal fibrosis.

scholarly journals Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method

2011 ◽  
Vol 35 (3) ◽  
pp. 221-226 ◽  
Author(s):  
Chih Kong Tong ◽  
Shalini Vellasamy ◽  
Boon Chong Tan ◽  
Maha Abdullah ◽  
Sharmili Vidyadaran ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Umbilical Cord Tissue

Human umbilical cord mesenchymal stem cell derived exosomes encapsulated in functional peptide hydrogels promote cardiac repair

2019 ◽  
Vol 7 (7) ◽  
pp. 2920-2933 ◽  
Author(s):  
Chaoshan Han ◽  
Jin Zhou ◽  
Chun Liang ◽  
Bin Liu ◽  
Xiangbin Pan ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Cardiac Repair ◽  
Human Umbilical Cord ◽  
Functional Peptide ◽  
Peptide Hydrogels

Stem cell-derived exosomes have been recognized as a potential therapy for cardiovascular disease.

scholarly journals Passage Effect on Aging of Human Umbilical Cord Derived Mesenchymal Stem Cell

2015 ◽  
Vol 15 (3) ◽  
pp. 170-177 ◽  
Author(s):  
Dian Mediana ◽  
Isabella Kurnia Liem ◽  
Jeanne Adiwinata Pawitan ◽  
Noviyanti Goei
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Passage Effect

Human Umbilical Cord Mesenchymal Stem Cell Therapy on Neuromyelitis Optica

2012 ◽  
Vol 9 (4) ◽  
pp. 250-255 ◽  
Author(s):  
Zhengjuan Lu ◽  
Dan Ye ◽  
Lai Qian ◽  
Lin Zhu ◽  
Chong Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Umbilical Cord ◽  
Neuromyelitis Optica ◽  
Stem Cell Therapy ◽  
Human Umbilical Cord ◽  
Mesenchymal Stem Cell Therapy
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