VAMP4 Controls Constitutive Recycling and Sorting of Post-Synaptic Receptors in Neuronal Dendrites

2021 ◽  
Author(s):  
May Bakr ◽  
Damien Jullié ◽  
Julia Krapivkina ◽  
Lou Bouit ◽  
Jennifer Petersen ◽  
...  
Keyword(s):  
Neuronal Dendrites ◽  
Synaptic Receptors

scholarly journals The vSNAREs VAMP2 and VAMP4 control recycling and intracellular sorting of post-synaptic receptors in neuronal dendrites

Cell Reports ◽  
2021 ◽  
Vol 36 (10) ◽  
pp. 109678
Author(s):  
May Bakr ◽  
Damien Jullié ◽  
Julia Krapivkina ◽  
Vincent Paget-Blanc ◽  
Lou Bouit ◽  
...  
Keyword(s):  
Neuronal Dendrites ◽  
Synaptic Receptors ◽  
Intracellular Sorting

scholarly journals A computational model of cell culture dynamics: the role of connectivity and synaptic receptors in the appearance of synchronized bursting events

2015 ◽  
Vol 16 (S1) ◽  
Author(s):  
Davide Lonardoni ◽  
Stefano Di Marco ◽  
Hayder Amin ◽  
Luca Berdondini ◽  
Thierry Nieus
Keyword(s):  
Cell Culture ◽  
Computational Model ◽  
Synaptic Receptors ◽  
Bursting Events

Effects of alcohol metabolic products on synaptic receptors

1990 ◽  
Vol 183 (2) ◽  
pp. 562
Author(s):  
Y. Nishizawa ◽  
S. Hahuka ◽  
S. Hayashi ◽  
T. Kurihara ◽  
Y. Takahashi
Keyword(s):  
Metabolic Products ◽  
Synaptic Receptors

scholarly journals Tonic Extrasynaptic GABAA Receptor Currents Control Gonadotropin-Releasing Hormone Neuron Excitability in the Mouse

Endocrinology ◽  
2011 ◽  
Vol 152 (4) ◽  
pp. 1551-1561 ◽  
Author(s):  
Janardhan P. Bhattarai ◽  
Seon Ah Park ◽  
Jin Bong Park ◽  
So Yeong Lee ◽  
Allan E. Herbison ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Gabaa Receptors ◽  
Brain Slice ◽  
Resting Membrane Potential ◽  
Receptor Signaling ◽  
Gaba Transporter ◽  
Gnrh Neurons ◽  
Inward Currents ◽  
Synaptic Receptors ◽  
Tonic Current

Abstract It is well established that the GABAA receptor plays an important role in regulating the electrical excitability of GnRH neurons. Two different modes of GABAA receptor signaling exist: one mediated by synaptic receptors generating fast (phasic) postsynaptic currents and the other mediated by extrasynaptic receptors generating a persistent (tonic) current. Using GABAA receptor antagonists picrotoxin, bicuculline methiodide, and gabazine, which differentiate between phasic and tonic signaling, we found that ∼50% of GnRH neurons exhibit an approximately 15-pA tonic GABAA receptor current in the acute brain slice preparation. The blockade of either neuronal (NO711) or glial (SNAP-5114) GABA transporter activity within the brain slice revealed the presence of tonic GABA signaling in ∼90% of GnRH neurons. The GABAA receptor δ subunit is only found in extrasynaptic GABAA receptors. Using single-cell RT-PCR, GABAA receptor δ subunit mRNA was identified in GnRH neurons and the δ subunit–specific agonist 4,5,6,7-tetrahydroisoxazolo [5,4-c] pyridin-3-ol was found to activate inward currents in GnRH neurons. Perforated-patch clamp studies showed that 4,5,6,7-tetrahydroisoxazolo [5,4-c] pyridin-3-ol exerted the same depolarizing or hyperpolarizing effects as GABA on juvenile and adult GnRH neurons and that tonic GABAA receptor signaling regulates resting membrane potential. Together, these studies reveal the presence of a tonic GABAA receptor current in GnRH neurons that controls their excitability. The level of tonic current is dependent, in part, on neuronal and glial GABA transporter activity and mediated by extrasynaptic δ subunit–containing GABAA receptors.

scholarly journals Disease-associated scaffold protein CNK2 modulates PSD size and influences trafficking of new synaptic binding partner TNIK

2019 ◽  
Author(s):  
Hanna L. Zieger ◽  
Stella-Amrei Kunde ◽  
Nils Rademacher ◽  
Bettina Schmerl ◽  
Sarah A. Shoichet
Keyword(s):  
Scaffold Protein ◽  
Subcellular Localisation ◽  
The Novel ◽  
Developmental Defects ◽  
Binding Partner ◽  
Neuronal Dendrites ◽  
Interaction Partners ◽  
Kinase Suppressor Of Ras ◽  
Synaptic Markers

AbstractScaffold proteins are responsible for structural organisation within cells; they form complexes with other proteins to facilitate signalling pathways and catalytic reactions. The scaffold protein connector enhancer of kinase suppressor of Ras 2 (CNK2) is predominantly expressed in neural tissues and was recently implicated in X-linked intellectual disability (ID). We have investigated the role of CNK2 in neurons in order to contribute to our understanding of how CNK2 alterations might cause developmental defects, and we have elucidated a functional role for CNK2 in the molecular processes that govern morphology of the postsynaptic density (PSD). We have also identified novel CNK2 interaction partners and explored their functional interdependency with CNK2. We focussed on the novel interaction partner TRAF2- and NCK-interacting kinase TNIK, which is also associated with ID. Both CNK2 and TNIK are expressed in neuronal dendrites and concentrated in dendritic spines, and staining with synaptic markers indicates a clear postsynaptic localisation. Importantly, our data highlight that CNK2 plays a role in directing TNIK subcellular localisation, and in neurons, CNK2 participates in ensuring that this multifunctional kinase is present at desirable levels at synaptic sites. In summary, our data indicate that CNK2 expression is critical for modulating PSD morphology; moreover, our study highlights a role for CNK2 in directing the localisation of regulatory proteins within the cell. Importantly, we describe a novel link between CNK2 and the regulatory kinase TNIK, and provide evidence supporting the idea that these proteins play complementary roles in the regulation of dendritic spine growth and maintenance.

scholarly journals Comprehensive catalog of dendritically localized mRNA isoforms from sub-cellular sequencing of single mouse neurons

2018 ◽  
Author(s):  
Sarah A. Middleton ◽  
James Eberwine ◽  
Junhyong Kim
Keyword(s):  
Hippocampal Neurons ◽  
Rna Localization ◽  
Synaptic Potentiation ◽  
Protein Structure Analysis ◽  
Mrna Isoforms ◽  
Neuronal Dendrites ◽  
Specific Localization ◽  
Dendritic Rna ◽  
Alternative Isoforms

AbstractRNA localization to neuronal dendrites is critical step for long-lasting synaptic potentiation, but there is little consensus regarding which RNAs are localized and the role of alternative isoforms in localization. Using independent RNA-sequencing from soma and dendrites of the same neuron, we deeply profiled the sub-cellular transcriptomes to assess the extent and variability of dendritic RNA localization in individual hippocampal neurons, including an assessment of differential localization of alternative 3’UTR isoforms. We identified 2,225 dendritic RNAs, including 298 cases of 3’UTR isoform-specific localization. We extensively analyzed the localized RNAs for potential localization motifs, finding that B1 and B2 SINE elements are up to 5.7 times more abundant in localized RNA 3’UTRs than non-localized, and also functionally characterized the localized RNAs using protein structure analysis. Finally, we integrate our list of localized RNAs with the literature to provide a comprehensive list of known dendritically localized RNAs as a resource.

scholarly journals Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments

2018 ◽  
Author(s):  
Anne-Sophie Hafner ◽  
Paul G. Donlin-Asp ◽  
Beulah Leitch ◽  
Etienne Herzog ◽  
Erin M. Schuman
Keyword(s):  
Protein Synthesis ◽  
Mouse Brain ◽  
Brain Slices ◽  
Metabolic Labeling ◽  
Presynaptic Terminals ◽  
Ample Evidence ◽  
Local Protein Synthesis ◽  
Axon Terminals ◽  
Neuronal Dendrites ◽  
Local Protein

AbstractThere is ample evidence for localized mRNAs and protein synthesis in neuronal dendrites, however, demonstrations of these processes in presynaptic terminals are limited. We used expansion microscopy to resolve pre- and postsynaptic compartments in brain slices. Most presynaptic terminals in the hippocampus and forebrain contained mRNA and ribosomes. We sorted fluorescently labeled synaptosomes from mouse brain and then sequenced hundreds of mRNA species present within excitatory boutons. After brief metabolic labeling, more them 30% of all presynaptic terminals exhibited a signal, providing evidence for ongoing protein synthesis. We tested different classic plasticity paradigms and observed unique patterns of rapid pre- and/or postsynaptic translation. Thus presynaptic terminals are translationally competent and local protein synthesis is differentially recruited to drive compartment-specific phenotypes that underlie different forms of plasticity.One sentence summaryProtein synthesis occurs in all synaptic compartments, including excitatory and inhibitory axon terminals.

Sign in / Sign up

Export Citation Format

Share Document