Efficient Delivery of Cytosolic Proteins by Protein-Hexahistidine-Metal Co-Assemblies

2021 ◽  
Author(s):  
Wenjuan Huang ◽  
Sijie Zhou ◽  
Bojiao Tang ◽  
Hongyan Xu ◽  
Xiaoxiao Wu ◽  
...  
Keyword(s):  
Cytosolic Proteins ◽  
Efficient Delivery

Efficient delivery of cytosolic proteins by protein-hexahistidine-metal co-assemblies

2021 ◽  
Author(s):  
Wenjuan Huang ◽  
Sijie Zhou ◽  
Bojiao Tang ◽  
Hongyan Xu ◽  
Xiaoxiao Wu ◽  
...  
Keyword(s):  
Cytosolic Proteins ◽  
Efficient Delivery

Design and Delivery of Therapeutic siRNAs: Application to MERS-Coronavirus

2018 ◽  
Vol 24 (1) ◽  
pp. 62-77 ◽  
Author(s):  
Sayed Sartaj Sohrab ◽  
Sherif Aly El-Kafrawy ◽  
Zeenat Mirza ◽  
Mohammad Amjad Kamal ◽  
Esam Ibraheem Azhar
Keyword(s):  
Enzymatic Degradation ◽  
Polymeric Nanoparticles ◽  
Genetic Disorders ◽  
Inorganic Nanoparticles ◽  
Innovative Technology ◽  
Viral Diseases ◽  
Viral Gene ◽  
Efficient Delivery ◽  
Disease Therapy ◽  
Target Sites

Background: The MERS-CoV is a novel human coronavirus causing respiratory syndrome since April 2012. The replication of MERS-CoV is mediated by ORF 1ab and viral gene activity can be modulated by RNAi approach. The inhibition of virus replication has been documented in cell culture against multiple viruses by RNAi approach. Currently, very few siRNA against MERS-CoV have been computationally designed and published. Methods: In this review, we have discussed the computational designing and delivery of potential siRNAs. Potential siRNA can be designed to silence a desired gene by considering many factors like target site, specificity, length and nucleotide content of siRNA, removal of potential off-target sites, toxicity and immunogenic responses. The efficient delivery of siRNAs into targeted cells faces many challenges like enzymatic degradation and quick clearance through renal system. The siRNA can be delivered using transfection, electroporation and viral gene transfer. Currently, siRNAs delivery has been improved by using advanced nanotechnology like lipid nanoparticles, inorganic nanoparticles and polymeric nanoparticles. Conclusion: The efficacy of siRNA-based therapeutics has been used not only against many viral diseases but also against non-viral diseases, cancer, dominant genetic disorders, and autoimmune disease. This innovative technology has attracted researchers, academia and pharmaceuticals industries towards designing and development of highly effective and targeted disease therapy. By using this technology, effective and potential siRNAs can be designed, delivered and their efficacy with toxic effects and immunogenic responses can be tested against MERS-CoV.

Binding Assay of Cytosolic Proteins to the Cytoskeleton

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (22) ◽  
Author(s):  
Stefano Del Duca ◽  
Giampiero Cai
Keyword(s):  
Binding Assay ◽  
Cytosolic Proteins

scholarly journals Modulation of Macrophages M1/M2 Polarization Using Carbohydrate-Functionalized Polymeric Nanoparticles

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 88
Author(s):  
Raquel G. D. Andrade ◽  
Bruno Reis ◽  
Benjamin Costas ◽  
Sofia A. Costa Lima ◽  
Salette Reis
Keyword(s):  
Inflammatory Responses ◽  
Polymeric Nanoparticles ◽  
Interaction Mechanism ◽  
Mannose Receptor ◽  
The Body ◽  
Receptor Expression ◽  
Production Methods ◽  
Polymeric Nanocarriers ◽  
Efficient Delivery

Exploiting surface endocytosis receptors using carbohydrate-conjugated nanocarriers brings outstanding approaches to an efficient delivery towards a specific target. Macrophages are cells of innate immunity found throughout the body. Plasticity of macrophages is evidenced by alterations in phenotypic polarization in response to stimuli, and is associated with changes in effector molecules, receptor expression, and cytokine profile. M1-polarized macrophages are involved in pro-inflammatory responses while M2 macrophages are capable of anti-inflammatory response and tissue repair. Modulation of macrophages’ activation state is an effective approach for several disease therapies, mediated by carbohydrate-coated nanocarriers. In this review, polymeric nanocarriers targeting macrophages are described in terms of production methods and conjugation strategies, highlighting the role of mannose receptor in the polarization of macrophages, and targeting approaches for infectious diseases, cancer immunotherapy, and prevention. Translation of this nanomedicine approach still requires further elucidation of the interaction mechanism between nanocarriers and macrophages towards clinical applications.

scholarly journals Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

2021 ◽  
Vol 22 (14) ◽  
pp. 7456
Author(s):  
Mousa A. Alghuthaymi ◽  
Aftab Ahmad ◽  
Zulqurnain Khan ◽  
Sultan Habibullah Khan ◽  
Farah K. Ahmed ◽  
...  
Keyword(s):  
Genome Editing ◽  
Viral Vectors ◽  
Polymeric Materials ◽  
Inorganic Nanoparticles ◽  
Plant Genome ◽  
Delivery Methods ◽  
Detailed Consideration ◽  
Crispr System ◽  
Efficient Delivery ◽  
Low Cytotoxicity

Rapid developments in the field of plant genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems necessitate more detailed consideration of the delivery of the CRISPR system into plants. Successful and safe editing of plant genomes is partly based on efficient delivery of the CRISPR system. Along with the use of plasmids and viral vectors as cargo material for genome editing, non-viral vectors have also been considered for delivery purposes. These non-viral vectors can be made of a variety of materials, including inorganic nanoparticles, carbon nanotubes, liposomes, and protein- and peptide-based nanoparticles, as well as nanoscale polymeric materials. They have a decreased immune response, an advantage over viral vectors, and offer additional flexibility in their design, allowing them to be functionalized and targeted to specific sites in a biological system with low cytotoxicity. This review is dedicated to describing the delivery methods of CRISPR system into plants with emphasis on the use of non-viral vectors.

Cytotoxicity Analysis of Nanoparticles by Association Rule Mining

2021 ◽  
Author(s):  
Gulsah Gul ◽  
Ramazan Yildirim ◽  
Nazar Ileri-Ercan
Keyword(s):  
Association Rule ◽  
Association Rule Mining ◽  
Long Term Effects ◽  
Rule Mining ◽  
Efficient Delivery ◽  
Intracellular Targets

Understanding the toxicity behavior of NPs is of great importance to ensure efficient delivery to intracellular targets without causing cytotoxicity, to measure the long-term effects of nanoparticles (NPs), and to...

scholarly journals Nanominerals: Fabrication Methods, Benefits and Hazards, and Their Applications in Ruminants with Special Reference to Selenium and Zinc Nanoparticles

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1916
Author(s):  
Sameh A. Abdelnour ◽  
Mahmoud Alagawany ◽  
Nesrein M. Hashem ◽  
Mayada R. Farag ◽  
Etab S. Alghamdi ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Nutrient Metabolism ◽  
Animal Products ◽  
Positive Effects ◽  
Ethical Implications ◽  
Livestock Sector ◽  
Efficient Delivery ◽  
Nano Zinc ◽  
Organic Minerals

Nanotechnology is one of the major advanced technologies applied in different fields, including agriculture, livestock, medicine, and food sectors. Nanomaterials can help maintain the sustainability of the livestock sector through improving quantitative and qualitative production of safe, healthy, and functional animal products. Given the diverse nanotechnology applications in the animal nutrition field, the use of nanomaterials opens the horizon of opportunities for enhancing feed utilization and efficiency in animal production. Nanotechnology facilitates the development of nano vehicles for nutrients (including trace minerals), allowing efficient delivery to improve digestion and absorption for better nutrient metabolism and physiology. Nanominerals are interesting alternatives for inorganic and organic minerals for animals that can substantially enhance the bioavailability and reduce pollution. Nanominerals promote antioxidant activity, and improve growth performance, reproductive performance, immune response, intestinal health, and the nutritional value of animal products. Nanominerals are also helpful for improving assisted reproductive technologies (ART) outcomes by enriching media for cryopreservation of spermatozoa, oocytes, and embryos with antioxidant nanominerals. Despite the promising positive effects of nanominerals on animal performance and health, there are various challenges related to nanominerals, including their metabolism and fate in the animal’s body. Thus, the economic, legal, and ethical implications of nanomaterials must also be considered by the authority. This review highlights the benefits of including nanominerals (particularly nano-selenium and nano-zinc) in animal diets and/or cryopreservation media, focusing on modes of action, physiological effects, and the potential toxicity of their impact on human health.

scholarly journals Nanoparticles as Drug Delivery Systems of RNAi in Cancer Therapy

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2380
Author(s):  
Diedie Li ◽  
Chengzhi Gao ◽  
Meiyan Kuang ◽  
Minhao Xu ◽  
Ben Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Therapeutic Strategy ◽  
Target Gene ◽  
Treatment Approach ◽  
Systemic Delivery ◽  
Efficient Delivery ◽  
Rnai Therapeutics ◽  
Cellular Machinery ◽  
Tumor Delivery

RNA interference (RNAi) can mediate gene-silencing by knocking down the expression of a target gene via cellular machinery with much higher efficiency in contrast to other antisense-based approaches which represents an emerging therapeutic strategy for combating cancer. Distinct characters of nanoparticles, such as distinctive size, are fundamental for the efficient delivery of RNAi therapeutics, allowing for higher targeting and safety. In this review, we present the mechanism of RNAi and briefly describe the hurdles and concerns of RNAi as a cancer treatment approach in systemic delivery. Furthermore, the current nanovectors for effective tumor delivery of RNAi therapeutics are classified, and the characteristics of different nanocarriers are summarized.

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