Sensitivity and Efficiency of RNA Sample Pooling for RT-qPCR Testing for SARS-CoV-2

2020 ◽  
Author(s):  
Leonelo E. Bautista ◽  
Luis A. Villar ◽  
Mario A. Cleves ◽  
Margarita Gelvez ◽  
Anyela Lozano-Parra ◽  
...  
Keyword(s):  
Sample Pooling

scholarly journals Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1166
Author(s):  
Immacolata Polvere ◽  
Elena Silvestri ◽  
Lina Sabatino ◽  
Antonia Giacco ◽  
Stefania Iervolino ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Virus Detection ◽  
Testing Procedures ◽  
Stem Infection ◽  
In Vitro Diagnostic ◽  
Sample Pooling ◽  
Pooling Strategy ◽  
Asymptomatic Individuals ◽  
The University

Since the beginning of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic, it has been clear that testing large groups of the population was the key to stem infection and prevent the effects of the coronavirus disease of 2019, mostly among sensitive patients. On the other hand, time and cost-sustainability of virus detection by molecular analysis such as reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) may be a major issue if testing is extended to large communities, mainly asymptomatic large communities. In this context, sample-pooling and test grouping could offer an effective solution. Here we report the screening on 1195 oral-nasopharyngeal swabs collected from students and staff of the Università degli Studi del Sannio (University of Sannio, Benevento, Campania, Italy) and analyzed by an in-house developed multiplex RT-qPCR for SARS-CoV-2 detection through a simple monodimensional sample pooling strategy. Overall, 400 distinct pools were generated and, within 24 h after swab collection, five positive samples were identified. Out of them, four were confirmed by using a commercially available kit suitable for in vitro diagnostic use (IVD). High accuracy, sensitivity and specificity were also determined by comparing our results with a reference IVD assay for all deconvoluted samples. Overall, we conducted 463 analyses instead of 1195, reducing testing resources by more than 60% without lengthening diagnosis time and without significant losses in sensitivity, suggesting that our strategy was successful in recognizing positive cases in a community of asymptomatic individuals with minor requirements of reagents and time when compared to normal testing procedures.

scholarly journals Evaluation of a Sample Pooling Strategy for Sars-cov-2 Using Real Time PCR

2021 ◽  
Author(s):  
Annet M Nankya ◽  
Luke Nyakarahuka ◽  
Stephen Balinandi ◽  
John Kayiwa ◽  
Julius Lutwama ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Virus Disease ◽  
Laboratory Diagnosis ◽  
Turnaround Time ◽  
Limited Resources ◽  
Asymptomatic Patients ◽  
Sample Pooling ◽  
Sample Testing ◽  
Pooling Strategy

Abstract Back ground: Corona Virus Disease 2019 (COVID 19) in Uganda was first reported in a male traveler from Dubai on 21st March, 2020 shortly after WHO had announced the condition as a global pandemic. Timely laboratory diagnosis of COVID -19 for all samples from both symptomatic and asymptomatic patients was observed as key in containing the pandemic and breaking the chain of transmission. However, there was a challenge of limited resources required for testing SARS-COV-2 in low and middle income countries. To mitigate this, a study was conducted to evaluate a sample pooling strategy for COVI-19 using real time PCR. The cost implication and the turn around time of pooled sample testing versus individual sample testing were also compared.Methods: In this study, 1260 randomly selected samples submitted to Uganda Virus Research Institute for analysis were batched in pools of 5, 10, and 15. The pools were then extracted using a Qiagen kit. Both individual and pooled RNA were screened for the SARS-COV-2 E gene using a Berlin kit. Results: Out of 1260 samples tested, 21 pools were positive in pools of 5 samples, 16 were positive in pools of 10 and 14 were positive in pools of 15 samples. The study also revealed that the pooling strategy helps to save a lot on resources, time and expands diagnostic capabilities without affecting the sensitivity of the test in areas with low SARS-COV-2 prevalence.Conclusion: This study demonstrated that the pooling strategy for COVID-19 reduced on the turnaround time and there was a substantial increase in the overall testing capacity with limited resources as compared to individual testing.

scholarly journals Correction to: On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Alemu Takele Assefa ◽  
Jo Vandesompele ◽  
Olivier Thas
Keyword(s):  
Rna Sequencing ◽  
Statistical Power ◽  
Sample Pooling

An amendment to this paper has been published and can be accessed via the original article.

High-throughput logP measurement using parallel liquid chromatography/ultraviolet/mass spectrometry and sample-pooling

2002 ◽  
Vol 16 (16) ◽  
pp. 1548-1555 ◽  
Author(s):  
Yining Zhao ◽  
Janan Jona ◽  
David T. Chow ◽  
Haojing Rong ◽  
David Semin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Throughput ◽  
Sample Pooling

Application of a sample pooling method for the accelerated assessment of the rate of uptake of drugs by the brain in rats

2006 ◽  
Vol 58 (6) ◽  
pp. 837-846 ◽  
Author(s):  
Kang-Pil Kim ◽  
Sung-Hoon Ahn ◽  
Jong-Hyuk Sung ◽  
Tae-Sung Koo ◽  
Myong-Joo Byun ◽  
...  
Keyword(s):  
Sample Pooling ◽  
The Brain

scholarly journals Blood biochemistry and the risk of cancer Effect of sample pooling

2004 ◽  
Vol 43 (7) ◽  
pp. 667-674 ◽  
Author(s):  
Matti Hakama ◽  
Timo Hakulinen ◽  
Michael G. Kenward ◽  
Ritva-kaarina Aaran ◽  
Arpo Aromaa ◽  
...  
Keyword(s):  
Blood Biochemistry ◽  
Sample Pooling ◽  
Risk Of Cancer
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