SIAH1 Promotes Granulosa Cells Senescence and Contributes to Premature Ovarian Failure by Reducing the Stability of TRF2

2020 ◽  
Author(s):  
Li Lin ◽  
Lu Chen ◽  
Wujiang Gao ◽  
Taoqiong Li ◽  
Chunli Sha ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Ovarian Failure ◽  
The Stability

scholarly journals Investigation of the Relation between Follicular Atresia and Granulosa Cells in Terms of Cell Death Mechanisms in Premature Ovarian Failure Model

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1529 ◽  
Author(s):  
Damla Akogullari ◽  
Elgin Turkoz Uluer ◽  
H. Seda Vatansever
Keyword(s):  
Cell Death ◽  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Ovarian Failure ◽  
Blood Levels ◽  
Failure Model ◽  
Follicular Atresia ◽  
Treatment Protocols ◽  
New Treatment ◽  
Cell Death Mechanisms

Premature Ovarian Failure; is characterized by the dysfunction or early depletion of ovarian reserves due to follicular loss in the ovary in women under age of 40. POF is the important cause of infertility and its etiology is still not clearly understood. Investigation of cell death mechanisms (CDM) that play a role in the follicular atresia (FA) triggered by excessive loss of granulosa cells (GCs) that provide metabolic support for oocyte and follicle development in the ovary will help to understand POF etiology. It was known that apoptosis and autophagy play a role in FA. Recent studies have shown that paraptosis, associated with endoplasmic reticulum stress (ERS), also exist in FA. POF model was established in C57BL/6 female mice by CTX and it was confirmed by increased follicle stimulating hormone (FSH), luteal hormone (LH) and decreased estradiol (E2) blood levels and follicle count. According to the results of the immunohistochemistry (IHC) cell death markers were significantly more expressed than control (C) and sham (S) groups in the POF model. In addition, more apoptotic cells were observed in the POF group compared to C and S in the TUNEL analysis. In consequence of this study apoptosis and autophagy as well as paraptosis play a role in the FA leading to POF, will help to develop new treatment protocols.

scholarly journals The Therapeutic Potential of Umbilical Cord Mesenchymal Stem Cells in Mice Premature Ovarian Failure

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Shufang Wang ◽  
Ling Yu ◽  
Min Sun ◽  
Sha Mu ◽  
Changyong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Ovarian Function ◽  
Treated Group ◽  
Ovarian Failure ◽  
Control Group ◽  
Wild Type

Mesenchymal stem cells, which are poorly immunogenic and have potent immunosuppressive activities, have emerged as promising cellular therapeutics for the treatment of several diseases. Mesenchymal-like cells derived from Wharton’s Jelly, called umbilical cord matrix stem cells (UCMSCs), reportedly secrete a variety of cytokines and growth factors, acting as trophic suppliers. Here, we used UCMSCs to treat premature ovarian failure (POF). Ovarian function was evaluated by ovulation and the number of follicles. Apoptosis of the granulosa cells (GC) was analyzed by TUNEL staining. We found that after transplantation of the UCMSCs, apoptosis of cumulus cells in the ovarian damage model was reduced and the function of the ovary had been recovered. The sex hormone level was significantly elevated in mice treated with UCMSCs. The number of follicles in the treated group was higher than in the control group. Our results demonstrate that UCMSCs can effectively restore ovary functionality and reduce apoptosis of granulosa cells. We compared the RNA expression of the UCMSCs treated group with the POF model and wild-type control group and found that the UCMSC group is most similar to the wild-type group. Our experiments provide new information regarding the treatment of ovarian function failure.

scholarly journals TRDMT1 participates in the DNA damage repair of granulosa cells in premature ovarian failure

Aging ◽  
2021 ◽  
Author(s):  
Chunli Sha ◽  
Lu Chen ◽  
Li Lin ◽  
Taoqiong Li ◽  
Hong Wei ◽  
...  
Keyword(s):  
Dna Damage ◽  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Dna Damage Repair ◽  
Ovarian Failure ◽  
Damage Repair

scholarly journals Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway

2019 ◽  
Vol 20 (24) ◽  
pp. 6311 ◽  
Author(s):  
Shiqi Wang ◽  
Shujun Lin ◽  
Mingmin Zhu ◽  
Chenglu Li ◽  
Shulian Chen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Akt Signaling ◽  
Ovarian Failure ◽  
Acupuncture Group ◽  
Female Rats ◽  
Akt Signaling Pathway ◽  
Expression Levels

Acupuncture is widely recognized as an effective therapy for premature ovarian failure (POF) in clinical, but information about its potential mechanisms is rarely explored. To investigate the mechanism, fifty SD female rats were randomly divided into normal group, POF group, POF+estradiol-valerate group (abbreviated as estradiol group), and POF+acupuncture group (abbreviated as acupuncture group). The estrous cycle of the rats was tracked by vaginal smears. Their ovaries morphology was observed by hematoxylin-eosin staining. The apoptotic level of granulosa cells was detected by in situ TUNEL fluorescence staining assay. Serum follicle-stimulating hormone (FSH) and estrogen (E2) levels were measured by enzyme-linked-immunosorbent-assay (ELISA). Protein and gene expression of PI3K, Akt, bcl-2, and bax were detected by Western blotting and qPCR. In the acupuncture and estradiol groups, compared with the POF group as controls, the apoptosis number of granulosa cells was significantly decreased (p < 0.05). FSH levels were decreased, while E2 levels were increased (p > 0.05). The gene and protein expression levels of PI3K, Akt, and bcl-2 were increased, while the expression levels of bax were decreased (p < 0.05), and the protein expression level of p-Akt increased. There was no significant difference between the acupuncture group and the estradiol group (p > 0.05). Acupuncture was able to regulate hormone levels in POF rats, up-regulate PI3K/Akt signaling pathway, and reduce the apoptosis of granulosa cells. This may be one of the mechanisms of acupuncture treating premature ovarian failure.

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