Follicular T Cells are Clonally and Transcriptionally Distinct in B Cell-Driven Autoimmune Disease

2020 ◽  
Author(s):  
Elliot Akama-Garren ◽  
Theo van den Broek ◽  
Lea Simoni ◽  
Carlos Castrillon ◽  
Cees van der Poel ◽  
...  
Keyword(s):  
T Cells ◽  
Autoimmune Disease ◽  
B Cell

scholarly journals Follicular T cells are clonally and transcriptionally distinct in B cell-driven mouse autoimmune disease

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Elliot H. Akama-Garren ◽  
Theo van den Broek ◽  
Lea Simoni ◽  
Carlos Castrillon ◽  
Cees E. van der Poel ◽  
...  
Keyword(s):  
T Cells ◽  
Autoimmune Disease ◽  
B Cell ◽  
Single Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Germinal Centers ◽  
Learning Approaches ◽  
Differential Gene ◽  
Disease Antigen

AbstractPathogenic autoantibodies contribute to tissue damage and clinical decline in autoimmune disease. Follicular T cells are central regulators of germinal centers, although their contribution to autoantibody-mediated disease remains unclear. Here we perform single cell RNA and T cell receptor (TCR) sequencing of follicular T cells in a mouse model of autoantibody-mediated disease, allowing for analyses of paired transcriptomes and unbiased TCRαβ repertoires at single cell resolution. A minority of clonotypes are preferentially shared amongst autoimmune follicular T cells and clonotypic expansion is associated with differential gene signatures in autoimmune disease. Antigen prediction using algorithmic and machine learning approaches indicates convergence towards shared specificities between non-autoimmune and autoimmune follicular T cells. However, differential autoimmune transcriptional signatures are preserved even amongst follicular T cells with shared predicted specificities. These results demonstrate that follicular T cells are phenotypically distinct in B cell-driven autoimmune disease, providing potential therapeutic targets to modulate autoantibody development.

scholarly journals CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease

2018 ◽  
Vol 201 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Kristen M. Valentine ◽  
Dan Davini ◽  
Travis J. Lawrence ◽  
Genevieve N. Mullins ◽  
Miguel Manansala ◽  
...  
Keyword(s):  
T Cells ◽  
Autoimmune Disease ◽  
B Cell ◽  
Class Switch ◽  
Cell Antibody ◽  
Antibody Class

scholarly journals The Current Researches on T Follicular Helper Cells and Associated Diseases

2012 ◽  
Vol 2 (2) ◽  
pp. 85-91
Author(s):  
Jianwei Zhou ◽  
Cui Kong
Keyword(s):  
T Cells ◽  
T Cell ◽  
Autoimmune Disease ◽  
Infectious Diseases ◽  
B Cell ◽  
Humoral Immunity ◽  
Cd4 T Cell ◽  
T Follicular Helper Cells ◽  
Tfh Cells ◽  
Follicular Helper

T follicular helper (Tfh) cell is a new subpopulation of CD4+ T cell family, whose differentiation is affected by Bcl-6, Blimp-1, STAT3, STAT5 and so on, and it could affect or decide the development of other subsets of CD4+ T cells. The important function of Tfh cell is  to help B cell mediate humoral immunity, many researches have proved that Tfh cells participate in the development of autoimmune disease, immunodeficient disease, tumor and    infectious diseases. DOI: http://dx.doi.org/10.3329/jemc.v2i2.12843 J Enam Med Col 2012; 2(2): 85-91

Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells

1987 ◽  
Vol 58 (03) ◽  
pp. 936-942 ◽  
Author(s):  
Lindsey A Miles ◽  
Edward F Plow
Keyword(s):  
T Cells ◽  
B Cell ◽  
Peripheral Blood ◽  
Binding Sites ◽  
Blood Cells ◽  
High Capacity ◽  
Red Cells ◽  
Peripheral Blood Cells ◽  
Cellular Functions ◽  
Fibrinolytic Proteins

SummaryGlu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound saturably and reversibly to monocytes, granulocytes and lymphocytes with apparent Kd values of 0.9-1.4 μM. The interactions were of high capacity with 1.6 to 49 × 105 sites/cell and involved the lysine binding sites of plasminogen. Both T cells and non-rosetting lymphocytes and two B cell lines saturably bound plasminogen. Urokinase bound saturably to gianulocytes, monocytes, non-rosetting lymphocytes and a B cell line, but minimally to T cells, platelets and red cells. Therefore, plasminogen binding sites of high capacity, of similar affinities, and with common recognition specificities are expressed by many peripheral blood cells. Urokinase receptors are also widely distributed, but less so than plasminogen binding sites. The binding ol plasminogen and/ or urokinase to these cells may lead to generation of cell- associated proteolytic activity which contributes to a variety of cellular functions.

B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds

2014 ◽  
Vol 17 (3) ◽  
pp. 421-426 ◽  
Author(s):  
B. Tokarz-Deptuła ◽  
P. Niedźwiedzka-Rystwej ◽  
B. Hukowska-Szematowicz ◽  
M. Adamiak ◽  
A. Trzeciak-Ryczek ◽  
...  
Keyword(s):  
T Cells ◽  
B Cells ◽  
B Cell ◽  
Cd8 T Cells ◽  
Peripheral Blood ◽  
Laboratory Animals ◽  
Immunological Parameters ◽  
Four Seasons ◽  
Mixed Breed ◽  
The Impact

Abstract In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.

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