Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs During Sperm Maturation in Drosophila

2020 ◽  
Author(s):  
Taichiro Iki ◽  
Moe Takami ◽  
Toshie Kai
Keyword(s):  
Sperm Maturation ◽  
Cyclophilin 40

Molecular Modeling and Dynamics Simulation Analysis of KATNAL1 for Identification of Novel Inhibitor of Sperm Maturation

2017 ◽  
Vol 20 (1) ◽  
pp. 82-92 ◽  
Author(s):  
Kishore Sarma ◽  
Shubhadeep Roychoudhury ◽  
Sudipta Bora ◽  
Budheswar Dehury ◽  
Pratap Parida ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Simulation Analysis ◽  
Sperm Maturation ◽  
Dynamics Simulation

Deficiency for Lcn8 causes epididymal sperm maturation defects in mice

2021 ◽  
Vol 548 ◽  
pp. 7-13
Author(s):  
Zongzhuang Wen ◽  
Dongyue Liu ◽  
Haixia Zhu ◽  
Xiaoyang Sun ◽  
Yu Xiao ◽  
...  
Keyword(s):  
Sperm Maturation ◽  
Epididymal Sperm

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

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