tRNA-Derived 5'tiRNAs are Essential for Embryonic Development by Facilitating tRNA Gene Transcription

2020 ◽  
Author(s):  
Luxi Chen ◽  
Wei Xu ◽  
Kunpeng Liu ◽  
Zheng Jiang ◽  
Yang Han ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Gene Transcription ◽  
Trna Gene

Relative Affinities of Nucleotide Substrates for the Yeast tRNA Gene Transcription Complex

1992 ◽  
Vol 47 (3-4) ◽  
pp. 320-322 ◽  
Author(s):  
Przemyslaw Szafranski ◽  
W. Jerzy Smagowicz
Keyword(s):  
Rna Polymerase ◽  
Gene Transcription ◽  
Trna Gene ◽  
Rna Polymerase Iii ◽  
Rna Polymerases ◽  
Yeast Trna ◽  
Polymerase Iii ◽  
Michaelis Constants ◽  
Auxiliary Protein

Abstract Apparent Michaelis constants for nucleotides in transcription of yeast tRN Agene by hom ologous RNA polymerase III with auxiliary protein factors, were found to be remarkably higher in initiation than in elongation of RNA chain. This supports presumptions regarding topological similarities between catalytic centers of bacterial and eukaryotic RNA polymerases.

Replication stress checkpoint signaling controls tRNA gene transcription

2010 ◽  
Vol 17 (8) ◽  
pp. 976-981 ◽  
Author(s):  
Vesna C Nguyen ◽  
Brett W Clelland ◽  
Darren J Hockman ◽  
Sonya L Kujat-Choy ◽  
Holly E Mewhort ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Trna Gene ◽  
Replication Stress ◽  
Checkpoint Signaling

scholarly journals Robust repression of tRNA gene transcription during stress requires protein arginine methylation

2019 ◽  
Vol 2 (3) ◽  
pp. e201800261 ◽  
Author(s):  
Richoo B Davis ◽  
Neah Likhite ◽  
Christopher A Jackson ◽  
Tao Liu ◽  
Michael C Yu
Keyword(s):  
Rna Polymerase ◽  
Gene Transcription ◽  
Protein Function ◽  
Transcriptional Repression ◽  
Trna Gene ◽  
Rna Polymerase Iii ◽  
Arginine Methylation ◽  
Major Protein ◽  
Polymerase Iii ◽  
Protein Arginine Methylation

Protein arginine methylation is an important means by which protein function can be regulated. In the budding yeast, this modification is catalyzed by the major protein arginine methyltransferase Hmt1. Here, we provide evidence that the Hmt1-mediated methylation of Rpc31, a subunit of RNA polymerase III, plays context-dependent roles in tRNA gene transcription: under conditions optimal for growth, it positively regulates tRNA gene transcription, and in the setting of stress, it promotes robust transcriptional repression. In the context of stress, methylation of Rpc31 allows for its optimal interaction with RNA polymerase III global repressor Maf1. Interestingly, mammalian Hmt1 homologue is able to methylate one of Rpc31’s human homologue, RPC32β, but not its paralogue, RPC32α. Our data led us to propose an efficient model whereby protein arginine methylation facilitates metabolic economy and coordinates protein-synthetic capacity.

A Composite Upstream Sequence Motif Potentiates tRNA Gene Transcription in Yeast

2003 ◽  
Vol 333 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Silvia Giuliodori ◽  
Riccardo Percudani ◽  
Priscilla Braglia ◽  
Roberto Ferrari ◽  
Elisa Guffanti ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Trna Gene ◽  
Sequence Motif ◽  
Upstream Sequence

scholarly journals Hepatitis B virus X protein induces RNA polymerase III-dependent gene transcription and increases cellular TATA-binding protein by activating the Ras signaling pathway.

1997 ◽  
Vol 17 (12) ◽  
pp. 6838-6846 ◽  
Author(s):  
H D Wang ◽  
A Trivedi ◽  
D L Johnson
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Rna Polymerase ◽  
Gene Transcription ◽  
Trna Gene ◽  
Rna Polymerase Iii ◽  
Tata Binding Protein ◽  
S2 Cells ◽  
B Virus ◽  
Pol Iii

Our previous studies have shown that the hepatitis B virus protein, X, activates all three classes of RNA polymerase III (pol III)-dependent promoters by increasing the cellular level of TATA-binding protein (TBP) (H.-D. Wang et al., Mol. Cell. Biol. 15:6720-6728, 1995), a limiting transcription component (A. Trivedi et al., Mol. Cell. Biol. 16:6909-6916, 1996). We have investigated whether these X-mediated events are dependent on the activation of the Ras/Raf-1 signaling pathway. Transient expression of a dominant-negative mutant Ras gene (Ras-ala15) in a Drosophila S-2 stable cell line expressing X (X-S2), or incubation of the cells with a Ras farnesylation inhibitor, specifically blocked both the X-dependent activation of a cotransfected tRNA gene and the increase in cellular TBP levels. Transient expression of a constitutively activated form of Ras (Ras-val12) in control S2 cells produced both an increase in tRNA gene transcription and an increase in cellular TBP levels. These events are not cell type specific since X-mediated gene induction was also shown to be dependent on Ras activation in a stable rat 1A cell line expressing X. Furthermore, increases in RNA pol III-dependent gene activity and TBP levels could be restored in X-S2 cells expressing Ras-ala15 by coexpressing a constitutively activated form of Raf-1. These events are serum dependent, and when the cells are serum deprived, the X-mediated effects are augmented. Together, these results demonstrate that the X-mediated induction of RNA pol III-dependent genes and increase in TBP are both dependent on the activation of the Ras/Raf-1 signaling cascade. In addition, these studies define two new and important consequences mediated by the activation of the Ras signal transduction pathway: an increase in the central transcription factor, TBP, and the induction of RNA pol III-dependent gene activity.

scholarly journals Two control regions for eukaryotic tRNA gene transcription.

1980 ◽  
Vol 77 (6) ◽  
pp. 3365-3368 ◽  
Author(s):  
D. DeFranco ◽  
O. Schmidt ◽  
D. Soll
Keyword(s):  
Gene Transcription ◽  
Trna Gene ◽  
Control Regions

scholarly journals Human Synaptobrevin-like 1 Gene Basal Transcription Is Regulated through the Interaction of Selenocysteine tRNA Gene Transcription Activating Factor-Zinc Finger 143 Factors with Evolutionary Conserved Cis-elements

2003 ◽  
Vol 279 (9) ◽  
pp. 7734-7739 ◽  
Author(s):  
Francesca Di Leva ◽  
Maria Immacolata Ferrante ◽  
Francesca Demarchi ◽  
Antonella Caravelli ◽  
Maria Rosaria Matarazzo ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Gene Transcription ◽  
Trna Gene ◽  
Cis Elements ◽  
Basal Transcription
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