Downregulated lncRNA NEAT1 Upregulates microRNA-381 to Induce Proliferation and Repress Apoptosis of Ovarian Granulosa Cells in Polycystic Ovarian Syndrome Rats by Inhibiting IGF1 Expression

2020 ◽  
Author(s):  
Jingran Zhen ◽  
Jiangli Li ◽  
Xia Li ◽  
Xue Wang ◽  
Yaling Xiao ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Polycystic Ovarian Syndrome ◽  
Ovarian Granulosa Cells ◽  
Igf1 Expression ◽  
Lncrna Neat1 ◽  
Ovarian Syndrome

scholarly journals MicroRNA-93 Promotes Ovarian Granulosa Cells Proliferation Through Targeting CDKN1A in Polycystic Ovarian Syndrome

2015 ◽  
Vol 100 (5) ◽  
pp. E729-E738 ◽  
Author(s):  
Linlin Jiang ◽  
Jia Huang ◽  
Lin Li ◽  
Yaxiao Chen ◽  
Xiaoli Chen ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Polycystic Ovarian Syndrome ◽  
Ovarian Granulosa Cells ◽  
Ovarian Syndrome

scholarly journals Long Noncoding RNA CTBP1-AS Regulates Ovarian Granulosa Cells Proliferation and Autophagy and Participates in Polycystic Ovary Syndrome

2021 ◽  
Author(s):  
Kaixuan Sun ◽  
Yinling Xiu ◽  
Jianbo Song ◽  
Yuexin Yu
Keyword(s):  
Granulosa Cells ◽  
Peripheral Blood ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Polycystic Ovary ◽  
Blood Leukocytes ◽  
Expression Levels ◽  
Ovarian Granulosa Cells ◽  
Related Proteins ◽  
Ovarian Syndrome

Abstract ObjectiveThis study aims to investigate the expression of long noncoding RNA CTBP1-AS in patients with polycystic ovarian syndrome (PCOS) and its effects on the proliferation and autophagy of ovarian granulosa cells. MethodsReal-time polymerase chain reaction assay was used to analyze the expression levels of CTBP1-AS in peripheral blood leukocytes of 40 PCOS patients and 40 non-PCOS women and the CTBP1-AS expression in ovarian granulosa cells and transfect ovarian granulosa cells with pcDNA3.1-CTBP1-AS and si-CTBP1-AS, respectively. Consequently, the CCK-8 kit was used to analyze the effect of CTBP1-AS on the proliferation of ovarian granulosa cells. Moreover, Western blotting was used to detect the expression levels of autophagy-related proteins LC3II/I and P62. ResultThe CTBP1-AS expression in the peripheral blood of PCOS patients was higher compared with non-PCOS patients (P < 0.05). Furthermore, the CTBP1-AS expression of ovarian granulosa cells in PCOS patients was higher compared with non-PCOS patients (P < 0.05). Consequently, CTBP1-AS overexpression in ovarian granulosa cells promotes the proliferation of ovarian granulosa cells and autophagy levels (P < 0.05). The CTBP1-AS expression interference in ovarian granulosa cells can inhibit the proliferation of ovarian granulosa cells and autophagy levels (P < 0.05). ConclusionThe CTBP1-AS expression in peripheral blood and ovarian granulosa cells of PCOS patients significantly increased, and CTBP1-AS could promote the proliferation of ovarian granulosa cells and the level of autophagy.

scholarly journals miR‑132 is upregulated in polycystic ovarian syndrome and inhibits granulosa cells viability by targeting Foxa1

2020 ◽  
Vol 22 (6) ◽  
pp. 5155-5162
Author(s):  
Xiangrong Cui ◽  
Xuan Jing ◽  
Junfen Liu ◽  
Xingyu Bi ◽  
Xueqing Wu
Keyword(s):  
Granulosa Cells ◽  
Polycystic Ovarian Syndrome ◽  
Ovarian Syndrome
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