Regulation of Intracellular Transition Metal Ion Level with a Ph-Sensitive Inorganic Nanocluster to Improve Therapeutic Angiogenesis by Enriching Conditioned Medium Retrieved from Human Adipose Derived Stem Cells

2020 ◽  
Author(s):  
Yeong Hwan Kim ◽  
Euiyoung Jung ◽  
Gwang-Bum Im ◽  
Yu-Jin Kim ◽  
Sun-Won Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transition Metal ◽  
Conditioned Medium ◽  
Metal Ion ◽  
Therapeutic Angiogenesis ◽  
Ph Sensitive ◽  
Adipose Derived Stem Cells ◽  
Transition Metal Ion

scholarly journals Regulation of intracellular transition metal ion level with a pH-sensitive inorganic nanocluster to improve therapeutic angiogenesis by enriching conditioned medium retrieved from human adipose derived stem cells

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yeong Hwan Kim ◽  
Euiyoung Jung ◽  
Gwang-Bum Im ◽  
Yu-Jin Kim ◽  
Sung-Won Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Transition Metal ◽  
Cell Therapy ◽  
Conditioned Medium ◽  
Molecular Mechanism ◽  
Therapeutic Efficacy ◽  
Metal Ion ◽  
Adipose Derived Stem Cells ◽  
Hindlimb Ischemia

Abstract Cell therapy based on human adipose derived stem cells (hADSCs) is a known potential therapeutic approach to induce angiogenesis in ischemic diseases. However, the therapeutic efficacy of direct hADSC injection is limited by a low cell viability and poor cell engraftment after administration. To improve the outcomes of this kind of approach, various types of nanoparticles have been utilized to improve the therapeutic efficacy of hADSC transplantation. Despite their advantages, the adverse effects of nanoparticles, such as genetic damage and potential oncogenesis based on non-degradable property of nanoparticles prohibit the application of nanoparticles toward the clinical applications. Herein, we designed a transition metal based inorganic nanocluster able of pH-selective degradation (ps-TNC), with the aim of enhancing an hADSC based treatment of mouse hindlimb ischemia. Our ps-TNC was designed to undergo degradation at low pH conditions, thus releasing metal ions only after endocytosis, in the endosome. To eliminate the limitations of both conventional hADSC injection and non-degradable property of nanoparticles, we have collected conditioned medium (CM) from the ps-TNC treated hADSCs and administrated it to the ischemic lesions. We found that intracellular increment of transition metal ion upregulated the hypoxia-inducible factor 1α, which can induce vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) expressions. Based on the molecular mechanism, the secretion of VEGF and bFGF by ps-TNC treated hADSCs showed a significant improvement compared to that of untreated cells. Injecting the CM collected from ps-TNC treated hADSCs into the mouse hindlimb ischemia model (ps-TNC-CM group) showed significantly improved angiogenesis in the lesions, with improved limb salvage and decreased muscle degeneration compared to the group injected with CM collected from normal hADSCs (CM group). This study suggests a novel strategy, combining a known angiogenesis molecular mechanism with both an improvement on conventional stem cell therapy and the circumvention of some limitations still present in modern approaches based on nanoparticles.

scholarly journals Efficacious and Clinically Relevant Conditioned Medium of Human Adipose-derived Stem Cells for Therapeutic Angiogenesis

2014 ◽  
Vol 22 (4) ◽  
pp. 862-872 ◽  
Author(s):  
Suk Ho Bhang ◽  
Seahyoung Lee ◽  
Jung-Youn Shin ◽  
Tae-Jin Lee ◽  
Hyeon-Ki Jang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Therapeutic Angiogenesis ◽  
Adipose Derived Stem Cells

Transition-metal ion-mediated morphological transformation of pyridine-based peptide nanostructures

2021 ◽  
Vol 45 (1) ◽  
pp. 153-161
Author(s):  
Narendra Singh ◽  
Ramesh Singh ◽  
Swati Sharma ◽  
Khushboo Kesharwani ◽  
Khashti Ballabh Joshi ◽  
...  
Keyword(s):  
Transition Metal ◽  
Metal Ion ◽  
Chemical Structure ◽  
Morphological Transformation ◽  
Transition Metal Ion

Pyridine-mediated constitutionally isomeric artificial metallopeptides possess remarkable advantages over the natural counterparts mainly due to their tailor-made chemical structure.

scholarly journals Comparison of divalent transition metal ion paraCEST MRI contrast agents

2013 ◽  
Vol 19 (2) ◽  
pp. 191-205 ◽  
Author(s):  
Sarina J. Dorazio ◽  
Abiola O. Olatunde ◽  
Pavel B. Tsitovich ◽  
Janet R. Morrow
Keyword(s):  
Transition Metal ◽  
Contrast Agents ◽  
Metal Ion ◽  
Mri Contrast Agents ◽  
Transition Metal Ion ◽  
Mri Contrast
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