Genetic Diversity and Multiplicity of Infection in Plasmodium Falciparum Isolates Causing Uncomplicated Malaria Infections in North-East India

2020 ◽  
Author(s):  
Ramzan Ahmed ◽  
Indu Sharma
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Uncomplicated Malaria ◽  
Multiplicity Of Infection ◽  
North East India ◽  
North East

Analysis of genetic diversity in Acorus calamus populations in South and North East India using ISSR markers

2012 ◽  
Vol 40 ◽  
pp. 156-161 ◽  
Author(s):  
V.K. Abdul Kareem ◽  
P.E. Rajasekharan ◽  
B.S. Ravish ◽  
S. Mini ◽  
Anuradha Sane ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Acorus Calamus ◽  
North East India ◽  
North East

Common Ancestry & Genetic Diversity of Few Indigenous Chilli Land Races of North East India

2018 ◽  
Vol 7 (6) ◽  
pp. 240-247
Author(s):  
Minakshi Bhattacharjee ◽  
Jutika Boro ◽  
Swati Singha ◽  
Hitesh Boro ◽  
Narbu Gengla ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Common Ancestry ◽  
Oxidation Reactions ◽  
Potential Health ◽  
North East India ◽  
North East ◽  
Rapd Profile ◽  
Dpph Method

Since chilies contain high quantities of bioactive potential compounds, they are expected to possess large number of pharmacological values like antioxi-dants, antifungal, antibacterial, anti-inflammatory, antiulcer, diuretics activi-ties and is being employed for the treatment of different ailments in the indigenous system of medicine. The current study aimed at comparative evaluation of antioxidant properties, phytochemicals and RAPD assay from extracts of 6 different types of chilli found in the states of Assam and Arunachal Pradesh of North East India. The plant extracts were screened for phyto-chemicals, antioxidant analysis and RAPD profile. Phytochemical analysis shows abundant presence of alkaloids, phenols, flavonoids, saponins etc. The antioxidants activities of all the aqueous extracts were evaluated using the DPPH method. The antioxidant activity (% oxidation inhibition) ranged from as high as 86.56% in Bhut Jolokia (Capsicum assamicum) extracts to as low as 18.77% in Badami Jolokia (Capsicum annum) extract. Chilli samples studied possess potential health benefits by inhibiting many oxidation reactions caused by free radicals. Genetic diversity in 6 chilli genotypes was analyzed by RAPD markers of the fourteen random primers among which 11 were polymorphic. The phylogenetic tree showed common ancestry and the divergence among the chilli species. Based on the current study findings, it may be concluded the RAPD technology can be a useful tool in explaining the genetic diversity among chilli genotypes.

scholarly journals Genetic diversity of Plasmodium falciparum and genetic profile in children affected by uncomplicated malaria in Cameroon

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Theresia Njuabe Metoh ◽  
Jun-Hu Chen ◽  
Philip Fon-Gah ◽  
Xia Zhou ◽  
Roger Moyou-Somo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Uncomplicated Malaria ◽  
Genetic Profile

Mitochondrial DNA-based genetic diversity of Anopheles nivipes in North East India

2015 ◽  
Vol 27 (6) ◽  
pp. 4236-4239 ◽  
Author(s):  
N. P. Sarma ◽  
S. Singh ◽  
D. K. Sarma ◽  
D. R. Bhattacharyya ◽  
M. C. Kalita ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
North East India ◽  
North East

scholarly journals Genetic Polymorphisms of Plasmodium Falciparum Isolates From Melka-werer, North East Ethiopia Based on the Merozoite Surface Protein-2 (msp-2) Gene as a Molecular Marker

2020 ◽  
Author(s):  
Hussein Mohammed Ali ◽  
Ashenafi Assefa ◽  
Melkie Chernet ◽  
Yonas Wulataw ◽  
Robert J Commons
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Molecular Marker ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Arid Area ◽  
Chain Reactions ◽  
Allelic Family ◽  
North East ◽  
Semi Arid

Abstract Background: The characterization of parasite populations circulating in malaria endemic areas is necessary to evaluate the success of ongoing interventions and malaria control strategies. This study was designed to investigate the genetic diversity of Plasmodium falciparum isolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (msp2) gene as a molecular marker. Methods: Dried blood spot isolates were collected from patients with Plasmodium falciparum infection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions for msp2. Results: 52 isolates were collected with msp2 identified in 41 (78.8%) isolates. Allele typing of the msp2 gene detected the 3D7/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 different msp2 genotypes were detected including 6 belonging to the 3D7/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96-1.42). The heterozygosity index was 0.50 for the msp2 locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p = 0.02).Conclusion: P. falciparum isolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.

scholarly journals Genomic miscellany and allelic frequencies of Plasmodium falciparum msp-1, msp-2 and glurp in parasite isolates

2021 ◽  
Author(s):  
Muzafar Shah ◽  
Ibrar Ullah ◽  
Sahib Gul Afridi ◽  
Muhammad Israr ◽  
Asifullah Khan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Local Health ◽  
Multiplicity Of Infection ◽  
High Genetic Diversity ◽  
Khyber Pakhtunkhwa ◽  
Chain Reactions ◽  
Allelic Family ◽  
Allelic Frequencies ◽  
The Impact

Plasmodium falciparum, the main causative agent of malaria is an important public health vector in Khyber Pakhtunkhwa, Pakistan. Identification of the genetic diversity of malaria parasites can inform the intensity of transmission and identify potential deficiencies in malaria control programs. The aim of this study was to investigate the genetic diversity, allele frequencies and multiplicity of infection (MOI) of P. falciparum in Khyber Pakhtunkhwa, Pakistan. Methods: A total of 85 isolates from patients presenting to the local health centers with P. falciparum species were collected from 2017 to 2019. Parasite DNA was extracted from a total of 200 micro litter whole blood per patient using the Qiagen DNA extraction kit according to manufactures instructions. The polymorphic region of msp-1, msp-2 and glurp loci were genotyped by using nested polymerase chain reactions followed by gel electrophoresis for fragment analysis. Results: Genetic diversity and allelic frequencies of msp-1, msp-2 and glurp were identified in 85 blood samples. A total of 62 msp alleles were detected in which 30 for msp-1 and 32 for msp-2. For msp-1 the successful amplification occurred in (75/85) 88.23% isolates for msp-1, 78.9% (67/85) for msp-2 and 70% (60/85) for glurp. For msp-1, the K1 allelic family was predominant at 66.66% (50/75), followed by RO33 and MAD20. The frequency of samples having only K1, MAD20 and RO33 were 21.34% (16/75), 8% (6/75) and 10.67% (8/75) respectively. In msp-2, the FC27 allelic family was the most abundant with 70.14% (47/67) compared to 3D7 with 67.16% (45/67). Nine glurp RII region genotypes were identified. The overall mean multiplicity of infection was 2.6 with1.8 and 1.4 for msp-1 and msp-2 respectively while for glurp RII genes (MOI=1.03). There was no significant association between multiplicity of infection and age group (Spearman rank coefficient = 0.050; P = 0.6). There was significant correlation between MOI and parasite density for msp-2 allelic family. Conclusion: Our study showed high genetic diversity and allelic frequency with multiple clones of msp-1, msp-2 and glurp in P. falciparum isolates from malaria patients in Khyber Pakhtunkhwa Pakistan. In the present study the genotype data provided the valuable information which is essential for monitoring the impact of malaria eradication efforts in this region.

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