Genome-Scale Profiling of Circulating Cell-Free DNA Signatures for Early Detection of Hepatocellular Carcinoma in Patients with Cirrhosis

2020 ◽  
Author(s):  
Lei Chen ◽  
Ghassan K. Abou-Alfa ◽  
Bo Zheng ◽  
Jing-Feng Liu ◽  
Jian Bai ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Early Detection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Genome Scale ◽  
Circulating Cell Free Dna

scholarly journals Erratum: Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients

Cell Research ◽  
2015 ◽  
Vol 25 (12) ◽  
pp. 1376-1376 ◽  
Author(s):  
Lu Wen ◽  
Jingyi Li ◽  
Huahu Guo ◽  
Xiaomeng Liu ◽  
Shengmin Zheng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cpg Islands ◽  
Cell Free Dna ◽  
Free Dna ◽  
Scale Detection ◽  
Genome Scale ◽  
Circulating Cell Free Dna

scholarly journals Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients

Cell Research ◽  
2015 ◽  
Vol 25 (11) ◽  
pp. 1250-1264 ◽  
Author(s):  
Lu Wen ◽  
Jingyi Li ◽  
Huahu Guo ◽  
Xiaomeng Liu ◽  
Shengmin Zheng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cpg Islands ◽  
Cell Free Dna ◽  
Free Dna ◽  
Scale Detection ◽  
Genome Scale ◽  
Circulating Cell Free Dna

scholarly journals Value of Quantitative and Qualitative Analyses of Circulating Cell-Free DNA as Diagnostic Tools for Hepatocellular Carcinoma

Medicine ◽  
2015 ◽  
Vol 94 (14) ◽  
pp. e722 ◽  
Author(s):  
Wenjun Liao ◽  
Yilei Mao ◽  
Penglei Ge ◽  
Huayu Yang ◽  
Haifeng Xu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Diagnostic Tools ◽  
Cell Free Dna ◽  
Free Dna ◽  
Qualitative Analyses ◽  
Circulating Cell Free Dna

scholarly journals Lengthening and shortening of plasma DNA in hepatocellular carcinoma patients

2015 ◽  
Vol 112 (11) ◽  
pp. E1317-E1325 ◽  
Author(s):  
Peiyong Jiang ◽  
Carol W. M. Chan ◽  
K. C. Allen Chan ◽  
Suk Hang Cheng ◽  
John Wong ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B ◽  
Molecular Diagnostic ◽  
Dna Molecules ◽  
Plasma Dna ◽  
Cell Free Dna ◽  
Free Dna ◽  
Size Profile ◽  
A Genome ◽  
Circulating Cell Free Dna

The analysis of tumor-derived circulating cell-free DNA opens up new possibilities for performing liquid biopsies for the assessment of solid tumors. Although its clinical potential has been increasingly recognized, many aspects of the biological characteristics of tumor-derived cell-free DNA remain unclear. With respect to the size profile of such plasma DNA molecules, a number of studies reported the finding of increased integrity of tumor-derived plasma DNA, whereas others found evidence to suggest that plasma DNA molecules released by tumors might be shorter. Here, we performed a detailed analysis of the size profiles of plasma DNA in 90 patients with hepatocellular carcinoma, 67 with chronic hepatitis B, 36 with hepatitis B-associated cirrhosis, and 32 healthy controls. We used massively parallel sequencing to achieve plasma DNA size measurement at single-base resolution and in a genome-wide manner. Tumor-derived plasma DNA molecules were further identified with the use of chromosome arm-levelz-score analysis (CAZA), which facilitated the studying of their specific size profiles. We showed that populations of aberrantly short and long DNA molecules existed in the plasma of patients with hepatocellular carcinoma. The short ones preferentially carried the tumor-associated copy number aberrations. We further showed that there were elevated amounts of plasma mitochondrial DNA in the plasma of hepatocellular carcinoma patients. Such molecules were much shorter than the nuclear DNA in plasma. These results have improved our understanding of the size profile of tumor-derived circulating cell-free DNA and might further enhance our ability to use plasma DNA as a molecular diagnostic tool.

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