scholarly journals A Metabolic Shift to Glycolysis Promotes Zebrafish Tail Regeneration Through TGF-β Dependent Dedifferentiation of Notochord Cells to Form the Blastema

2020 ◽  
Author(s):  
Jason W. Sinclair ◽  
David R. Hoying ◽  
Erica Bresciani ◽  
Damian Dalle Nogare ◽  
Carli D. Needle ◽  
...  
Keyword(s):  
Metabolic Shift ◽  
Tail Regeneration ◽  
Notochord Cells

scholarly journals A metabolic shift to glycolysis promotes zebrafish tail regeneration through TGF–β dependent dedifferentiation of notochord cells to form the blastema

2020 ◽  
Author(s):  
Jason W. Sinclair ◽  
David R. Hoying ◽  
Erica Bresciani ◽  
Damian Dalle Nogare ◽  
Carli D. Needle ◽  
...  
Keyword(s):  
Single Cell Analysis ◽  
High Capacity ◽  
Metabolic Shift ◽  
Cell Analysis ◽  
Metabolic Switch ◽  
Tail Regeneration ◽  
Blastema Formation ◽  
Larval Zebrafish ◽  
Notochord Cells ◽  
Site Blocking

AbstractMammals are generally poor at tissue regeneration, in contrast, fish maintain a high capacity for regenerating complex tissues after injury. Using larval zebrafish, we show that tail amputation triggers an metabolic shift to glycolysis in cells surrounding the notochord as they reposition to the amputation site. Blocking glycolysis prevents the fin from regenerating after amputation due to the failure to form a normal, pluripotent blastema. We performed a time series of scRNA-sequencing on regenerating tails under normal conditions or in the absence of glycolysis. Strikingly, we detected a transient cell population in the single cell analysis that represents notochord sheath cells undergoing a TGF–β dependent dedifferentiation and epithelium-to-mesenchyme transition to become pluripotent blastema cells. We further demonstrated that the metabolic switch to glycolysis is required for TGF–β signaling and blocking either glycolysis or TGF–β receptors results in aberrant blastema formation through the suppression of essential EMT mediators such as snai1.

scholarly journals Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells

2018 ◽  
Author(s):  
Maria Montserrat Garcia Romero ◽  
Gareth McCathie ◽  
Philip Jankun ◽  
Henry Hamilton Roehl
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Tail Regeneration ◽  
Damage Site ◽  
Hedgehog Signalling ◽  
Ability To Regenerate ◽  
Aquatic Vertebrates ◽  
Notochord Cells ◽  
Developmental Signalling

AbstractAquatic vertebrates have a remarkable ability to regenerate limbs and tails after amputation. Previous studies indicate that reactive oxygen species (ROS) signaling initiates regeneration, but the mechanism by which this takes place is poorly understood. Developmental signalling pathways have been shown to have pro-regenerative roles in many systems. However, whether these are playing roles that are specific to regeneration, or are simply recapitulating their developmental functions is unclear. We have analysed zebrafish larval tail regeneration and find evidence that ROS released upon wounding cause repositioning of notochord cells to the damage site. These cells secrete Hedgehog ligands which are required for regeneration. Hedgehog signalling is not required for normal tail development suggesting that it has a regeneration specific role. Our results provide a model for how ROS initiate tail regeneration, and indicate that developmental signaling pathways can play regenerative functions that are not directly related to their developmental roles.

scholarly journals Correction to: Evolutionary divergence in tail regeneration between Xenopus laevis and Xenopus tropicalis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shouhong Wang ◽  
Yun-Bo Shi
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Tropicalis ◽  
Evolutionary Divergence ◽  
Tail Regeneration

An amendment to this paper has been published and can be accessed via the original article.

scholarly journals Reconstitution of the central and peripheral nervous system during salamander tail regeneration

2012 ◽  
Vol 109 (34) ◽  
pp. E2258-E2266 ◽  
Author(s):  
L. Mchedlishvili ◽  
V. Mazurov ◽  
K. S. Grassme ◽  
K. Goehler ◽  
B. Robl ◽  
...  
Keyword(s):  
Nervous System ◽  
Peripheral Nervous System ◽  
Tail Regeneration

scholarly journals Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Elavarasan Subramani ◽  
Arun Prabhu Rameshbabu ◽  
Manivannan Jothiramajayam ◽  
Bhuvaneshwaran Subramanian ◽  
Debangana Chakravorty ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Stromal Cells ◽  
Metabolic Shift ◽  
Endometrial Stromal Cells ◽  
Heat Shock Protein 65

Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior

2017 ◽  
Vol 18 (12) ◽  
pp. 1342-1352 ◽  
Author(s):  
Michio Miyajima ◽  
Baihao Zhang ◽  
Yuki Sugiura ◽  
Kazuhiro Sonomura ◽  
Matteo M Guerrini ◽  
...  
Keyword(s):  
T Cells ◽  
Emotional Behavior ◽  
Metabolic Shift ◽  
Brain Monoamines ◽  
Deficient Mice
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