Cx43-Mediated Bone Marrow Stromal Cells Affect the Imatinib Resistance of K562 Cells Via Ca 2+-Dependent Gap Junction Intercellular Communication (GJIC)

2019 ◽  
Author(s):  
Xiaoping Li ◽  
Rui Wang ◽  
Shijie Yang ◽  
Yi Deng ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Gap Junction ◽  
Intercellular Communication ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
K562 Cells ◽  
Marrow Stromal Cells ◽  
Imatinib Resistance ◽  
Gap Junction Intercellular Communication

scholarly journals Gap Junction–Mediated Import of MicroRNA from Bone Marrow Stromal Cells Can Elicit Cell Cycle Quiescence in Breast Cancer Cells

2011 ◽  
Vol 71 (5) ◽  
pp. 1550-1560 ◽  
Author(s):  
Philip K. Lim ◽  
Sarah A. Bliss ◽  
Shyam A. Patel ◽  
Marcelo Taborga ◽  
Meneka A. Dave ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Bone Marrow ◽  
Gap Junction ◽  
Cancer Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Breast Cancer Cells ◽  
Marrow Stromal Cells

Human Marrow Stromal Cells Enhance Connexin43 Gap Junction Intercellular Communication in Cultured Astrocytes

2005 ◽  
Vol 14 (2-3) ◽  
pp. 109-117 ◽  
Author(s):  
Qi Gao ◽  
Mark Katakowski ◽  
Xiaoguang Chen ◽  
Yi Li ◽  
Michael Chopp
Keyword(s):  
Gap Junction ◽  
Intercellular Communication ◽  
Stromal Cells ◽  
Connexin 43 ◽  
Marrow Stromal Cells ◽  
Dye Transfer ◽  
Cx43 Expression ◽  
Soluble Factors ◽  
Functional Benefit ◽  
Gap Junction Intercellular Communication

Human marrow stromal cells (hMSCs) provide functional benefit in rats subjected to stroke. Astrocytes are coupled into a cellular network via gap junction channels, predominantly composed of connexin-43 (Cx43) proteins. Astrocytes are believed to play a vital role in neuroprotection by providing energy substrates to neurons and by regulating the concentrations of K+ and neurotransmitters via gap junctions. We therefore investigated the effect of factors secreted by hMSCs on gap junction intercellular communication (GJIC), expression of Cx43, and phosphorylation of Cx43 in an astrocyte cell culture system. Exposing rat cortical astrocytes to various concentrations of hMSC conditioned medium, we demonstrate that hMSCs produce soluble factors that significantly increase astrocytic GJIC, measured by the scrape-loading dye transfer method. Immunohistochemistry and Western blot showed increased Cx43 expression concomitant with altered GJIC. As the PI3K/Akt signaling pathway has been demonstrated to alter gap junction expression and GJIC, we selectively blocked phosphoinositide 3-kinase (PI3K). Addition of the PI3K inhibitor LY294002 decreased GJIC and Cx43 expression in astrocytes. These inhibitory effects of LY294002 were countered by the addition of hMSC conditioned media. Furthermore, coculturing hMSCs with rat astrocytes increased astrocyte GJIC in a manner dependent upon the hMSC/astrocyte ratio. These findings demonstrate that hMSCs secrete soluble factors that increase GJIC of astrocytes through upregulation of Cx43, and indicate a mechanistic role for PI3K.

scholarly journals Differentiation of Bone Marrow Stromal Cells Into the Cardiac Phenotype Requires Intercellular Communication With Myocytes

Circulation ◽  
2004 ◽  
Vol 110 (17) ◽  
pp. 2658-2665 ◽  
Author(s):  
Meifeng Xu ◽  
Maqsood Wani ◽  
Yan-Shan Dai ◽  
Jiang Wang ◽  
Mei Yan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intercellular Communication ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Cardiac Phenotype

scholarly journals Connexin43 intercellular communication drives the early differentiation of human bone marrow stromal cells into osteoblasts

2017 ◽  
Vol 233 (2) ◽  
pp. 946-957 ◽  
Author(s):  
Julie Talbot ◽  
Régis Brion ◽  
Audrey Lamora ◽  
Mathilde Mullard ◽  
Sarah Morice ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intercellular Communication ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Early Differentiation
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