AMPK Activation by Ozone Therapy Inhibits Tissue Factor-Triggered Intestinal Ischemia and Ameliorates Chemotherapeutic Enteritis

2019 ◽  
Author(s):  
Qingqing Yu ◽  
Chen Zhang ◽  
Xing Yang ◽  
Xiaotao Zhang ◽  
Chaoyu Wang ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Intestinal Ischemia ◽  
Ampk Activation ◽  
Ozone Therapy

scholarly journals AMPK activation by ozone therapy inhibits tissue factor‐triggered intestinal ischemia and ameliorates chemotherapeutic enteritis

2020 ◽  
Vol 34 (9) ◽  
pp. 13005-13021
Author(s):  
Qingqing Yu ◽  
Xing Yang ◽  
Chen Zhang ◽  
Xiaotao Zhang ◽  
Chaoyu Wang ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Intestinal Ischemia ◽  
Ampk Activation ◽  
Ozone Therapy

scholarly journals The Level of Certain Interleukins and Tissue Factor in Conditions of Intestinal Ischemia and Reperfusion in Rats

2020 ◽  
Vol 10 (4) ◽  
pp. 416-420
Author(s):  
Zoya Artamonova ◽  
Evgeny Namokonov ◽  
Marina Darenskaya ◽  
Natalya Semenova ◽  
Lyubov Kolesnikova ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Mesenteric Ischemia ◽  
Intestinal Ischemia ◽  
Acute Mesenteric Ischemia ◽  
Male Rats ◽  
Blood Levels ◽  
Time Intervals ◽  
Rat Blood ◽  
Laboratory Markers ◽  
Systemic Inflammatory Reaction

Background: The aim of this study was to estimate the blood levels of interleukins (ILs) and tissue factor (TF) in rat blood when modeling acute mesenteric ischemia (AMI) in various stages and during reperfusion. Methods and Results: The study was performed on white non-linear male rats, weighing 200±25g. AMI was simulated by ligating the cranial mesenteric artery at the mesentery root. Then, at certain time intervals, a relaparotomy was performed, blood was collected, and the animals were subjected to reperfusion at different time intervals. During the experiment, the animals were divided into 3 groups depending on the time of ischemia (3 hours [n=10], 6 hours [n=10], and 8 hours [n=10]) followed by reperfusion after the indicated time of ischemia. The level of ILs (IL-6, IL-8, and IL-10) and TF in rat blood serum was determined by EIA. We found that AMI in rats is accompanied by pronounced changes in the immune system and the development of a systemic inflammatory reaction syndrome, which is aggravated by reperfusion. At the same time, the restoration of blood flow after 3-hour ischemia was characterized by an increase in the concentration of IL-6, IL-8, and IL-10; after 6-hour ischemia – by an increase in the IL-6, IL-8 content and TF, and stabilization of the IL-10 concentration. Reperfusion after 8-hour ischemia was accompanied by an increase in the IL-6 concentration, a decrease in the levels of IL-8, IL-10, and TF, which can lead to the progression of necrotic changes in the intestine. Conclusion: The detected changes can serve as laboratory markers that characterize the course and stage of acute mesenteric ischemia.

Two isoprenylated flavonoids from Dorstenia psilurus activate AMPK, stimulate glucose uptake, inhibit glucose production and lower glycemia

2019 ◽  
Vol 476 (24) ◽  
pp. 3687-3704 ◽  
Author(s):  
Aphrodite T. Choumessi ◽  
Manuel Johanns ◽  
Claire Beaufay ◽  
Marie-France Herent ◽  
Vincent Stroobant ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Human Cancer ◽  
Glucose Production ◽  
Liver Mitochondria ◽  
New Drugs ◽  
Structural Isomer ◽  
Rat Liver Mitochondria ◽  
Ionization Mass ◽  
Ampk Activation ◽  
Starting Point

Root extracts of a Cameroon medicinal plant, Dorstenia psilurus, were purified by screening for AMP-activated protein kinase (AMPK) activation in incubated mouse embryo fibroblasts (MEFs). Two isoprenylated flavones that activated AMPK were isolated. Compound 1 was identified as artelasticin by high-resolution electrospray ionization mass spectrometry and 2D-NMR while its structural isomer, compound 2, was isolated for the first time and differed only by the position of one double bond on one isoprenyl substituent. Treatment of MEFs with purified compound 1 or compound 2 led to rapid and robust AMPK activation at low micromolar concentrations and increased the intracellular AMP:ATP ratio. In oxygen consumption experiments on isolated rat liver mitochondria, compound 1 and compound 2 inhibited complex II of the electron transport chain and in freeze–thawed mitochondria succinate dehydrogenase was inhibited. In incubated rat skeletal muscles, both compounds activated AMPK and stimulated glucose uptake. Moreover, these effects were lost in muscles pre-incubated with AMPK inhibitor SBI-0206965, suggesting AMPK dependency. Incubation of mouse hepatocytes with compound 1 or compound 2 led to AMPK activation, but glucose production was decreased in hepatocytes from both wild-type and AMPKβ1−/− mice, suggesting that this effect was not AMPK-dependent. However, when administered intraperitoneally to high-fat diet-induced insulin-resistant mice, compound 1 and compound 2 had blood glucose-lowering effects. In addition, compound 1 and compound 2 reduced the viability of several human cancer cells in culture. The flavonoids we have identified could be a starting point for the development of new drugs to treat type 2 diabetes.

Mid-aortic syndrome: computed tomography angiography depicting extensive collateral circulation in an 11-year-old patient with thoracoabdominal aortic coarctation

VASA ◽  
2012 ◽  
Vol 41 (2) ◽  
pp. 132-135 ◽  
Author(s):  
Krohn ◽  
Gebauer ◽  
Hübler ◽  
Beck
Keyword(s):  
Computed Tomography ◽  
Computed Tomography Angiography ◽  
Collateral Circulation ◽  
Intestinal Ischemia ◽  
Clinical Presentation ◽  
Lower Limbs ◽  
Upper Body ◽  
Descending Aorta ◽  
Before And After ◽  
Blood Pressures

The mid-aortic syndrome is an uncommon clinical condition characterized by severe narrowing of the descending aorta, usually with involvement of its renal and visceral branches, presenting with uncontrollably elevated blood pressures of the upper body, renal and cardiac failure, intestinal ischemia, encephalopathy symptoms and claudication of the lower limbs, although clinical presentation is variable. In this article we report the case of an eleven-year-old patient with the initial diagnosis of a mid-aortic syndrome and present the computed tomography angiography pictures and reconstructions before and after surgical therapy.

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