Making a Hydrophilic Interfacial Scaffold with Hierarchical Nanofibre Architecture to Enhance the Phenotypic Expression of Epithelial and Smooth Muscle Cells for Urethral Reconstruction

2019 ◽  
Author(s):  
Yuqing Niu ◽  
Guochang Liu ◽  
Ming Fu ◽  
Feng Li ◽  
Wen Fu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Phenotypic Expression ◽  
Muscle Cells ◽  
Urethral Reconstruction

Urethral reconstruction using an amphiphilic tissue-engineered autologous polyurethane nanofiber scaffold with rapid vascularization function

2020 ◽  
Vol 8 (8) ◽  
pp. 2164-2174
Author(s):  
Yuqing Niu ◽  
Guochang Liu ◽  
Chuangbi Chen ◽  
Ming Fu ◽  
Wen Fu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cells ◽  
Smooth Muscle Cells ◽  
Phenotypic Expression ◽  
Muscle Cells ◽  
Urethral Reconstruction ◽  
Nanofiber Scaffold

We report the efficient application of a well-layered tubular amphiphilic nanofiber of a polyurethane copolymer (PU-ran) for the regulation the phenotypic expression of epithelial cells (ECs) and smooth muscle cells (SMCs) for vascularized urethral reconstruction.

Stiffness of Aligned Fibers Regulates the Phenotypic Expression of Vascular Smooth Muscle Cells

2019 ◽  
Vol 11 (7) ◽  
pp. 6867-6880 ◽  
Author(s):  
Bingcheng Yi ◽  
Yanbing Shen ◽  
Han Tang ◽  
Xianliu Wang ◽  
Bin Li ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Phenotypic Expression ◽  
Muscle Cells ◽  
Aligned Fibers

Phenotypic changes of human aortic smooth muscle cells during development and in the adult vessel

1991 ◽  
Vol 261 (4) ◽  
pp. L78-L80
Author(s):  
Marina A. Glukhova ◽  
Maria G. Frid ◽  
Victor E. Koteliansky
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Phenotypic Expression ◽  
Muscle Cells ◽  
Cytoskeletal Proteins ◽  
Myosin Heavy Chains ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Heavy Chains ◽  
Aortic Media

To characterize phenotypic expression of human aortic smooth muscle cells (SMCs), we have studied the content of cytodifferentiation-related cytoskeletal proteins, and of fibronectin (FN) variants in the samples of media from the fetal, child, and adult aorta and in the subendothelial intima of the normal and atherosclerotic aorta. Mature SMCs from the adult aortic media contained high amounts of agr-SM-actin, SM-myosin heavy chains, meta-vinculin, and 150 kDa caldesmon. Cytokeratin 8 and extra domain-containing variants of FN (A-FN and B-FN) were not found in these cells. The SMCs from the aortic media of 10-wk-old fetus contained low amounts of the SM markers, expressed cytokeratin 8, A-FN, and B-FN. In 25-wk-old fetus, as well as in 2- and 6-mo-old child, aortic medial SMCs expressed an intermediate phenotype, and only in 18-mo-old child were the cells found to be similar to those from adult media. SMCs from the normal adult subendothelial intima contained reduced amounts of meta-vinculin and of 150 kDa caldesmon, and they expressed A-FN. In addition, the SMCs from atherosclerotic fibrous plaque contained a decreased proportion of agr-SM-actin and of SM-myosin heavy chains, whereas cytokeratin 8 was found. Therefore we conclude that the SMCs from intimal thickenings appear to express a less mature phenotype than that of the medial cells from adult aorta. Rather, these SMCs contain reduced amounts of the SM markers and express proteins typical of the fetal SMC phenotype, A-FN and cytokeratin 8. cytodifferentiation; cytoskeletal proteins; fibronectin variant forms

Phenotypic changes of human aortic smooth muscle cells during development and in the adult vessel

1991 ◽  
Vol 261 (4) ◽  
pp. 78-80 ◽  
Author(s):  
Marina A. Glukhova ◽  
Maria G. Frid ◽  
Victor E. Koteliansky
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Phenotypic Expression ◽  
Muscle Cells ◽  
Cytoskeletal Proteins ◽  
Myosin Heavy Chains ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Heavy Chains ◽  
Aortic Media

To characterize phenotypic expression of human aortic smooth muscle cells (SMCs), we have studied the content of cytodifferentiation-related cytoskeletal proteins, and of fibronectin (FN) variants in the samples of media from the fetal, child, and adult aorta and in the subendothelial intima of the normal and atherosclerotic aorta. Mature SMCs from the adult aortic media contained high amounts of -SM-actin, SM-myosin heavy chains, meta-vinculin, and 150 kDa caldesmon. Cytokeratin 8 and extra domain-containing variants of FN (A-FN and B-FN) were not found in these cells. The SMCs from the aortic media of 10-wk-old fetus contained low amounts of the SM markers, expressed cytokeratin 8, A-FN, and B-FN. In 25-wk-old fetus, as well as in 2- and 6-mo-old child, aortic medial SMCs expressed an intermediate phenotype, and only in 18-mo-old child were the cells found to be similar to those from adult media. SMCs from the normal adult subendothelial intima contained reduced amounts of meta-vinculin and of 150 kDa caldesmon, and they expressed A-FN. In addition, the SMCs from atherosclerotic fibrous plaque contained a decreased proportion of -SM-actin and of SM-myosin heavy chains, whereas cytokeratin 8 was found. Therefore we conclude that the SMCs from intimal thickenings appear to express a less mature phenotype than that of the medial cells from adult aorta. Rather, these SMCs contain reduced amounts of the SM markers and express proteins typical of the fetal SMC phenotype, A-FN and cytokeratin 8. cytodifferentiation; cytoskeletal proteins; fibronectin variant forms

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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