Large Fragment Deletions Induced by Cas9 Cleavage While Not in BEs System in Rabbit

2019 ◽  
Author(s):  
Yuning Song ◽  
Zhiquan Liu ◽  
Yuxin Zhang ◽  
Mao Chen ◽  
Tingting Sui ◽  
...  
Keyword(s):  
Large Fragment

scholarly journals Split-wrmScarlet and split-sfGFP: Tools for faster, easier fluorescent l abeling of endogenous proteins in Caenorhabditis elegans

Genetics ◽  
2021 ◽  
Author(s):  
Jérôme Goudeau ◽  
Catherine S Sharp ◽  
Jonathan Paw ◽  
Laura Savy ◽  
Manuel D Leonetti ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Large Fragment ◽  
C Elegans ◽  
High Affinity ◽  
Red Fluorescent Proteins ◽  
Invaluable Tool ◽  
Endogenous Proteins ◽  
Fluorescent Tags

Abstract We create and share a new red fluorophore, along with a set of strains, reagents and protocols, to make it faster and easier to label endogenous C. elegans proteins with fluorescent tags. CRISPR-mediated fluorescent labeling of C. elegans proteins is an invaluable tool, but it is much more difficult to insert fluorophore-size DNA segments than it is to make small gene edits. In principle, high-affinity asymmetrically split fluorescent proteins solve this problem in C. elegans: the small fragment can quickly and easily be fused to almost any protein of interest, and can be detected wherever the large fragment is expressed and complemented. However, there is currently only one available strain stably expressing the large fragment of a split fluorescent protein, restricting this solution to a single tissue (the germline) in the highly autofluorescent green channel. No available C. elegans lines express unbound large fragments of split red fluorescent proteins, and even state-of-the-art split red fluorescent proteins are dim compared to the canonical split-sfGFP protein. In this study, we engineer a bright, high-affinity new split red fluorophore, split-wrmScarlet. We generate transgenic C. elegans lines to allow easy single-color labeling in muscle or germline cells and dual-color labeling in somatic cells. We also describe a novel expression strategy for the germline, where traditional expression strategies struggle. We validate these strains by targeting split-wrmScarlet to several genes whose products label distinct organelles, and we provide a protocol for easy, cloning-free CRISPR/Cas9 editing. As the collection of split-FP strains for labeling in different tissues or organelles expands, we will post updates at doi.org/10.5281/zenodo.3993663

Site-specific modification of E. coli DNA polymerase I large fragment with pyridoxal 5'-phosphate

Biochemistry ◽  
1984 ◽  
Vol 23 (9) ◽  
pp. 2073-2078 ◽  
Author(s):  
Anup K. Hazra ◽  
Sevilla Detera-Wadleigh ◽  
Samuel H. Wilson
Keyword(s):  
Dna Polymerase ◽  
Large Fragment ◽  
Dna Polymerase I ◽  
Site Specific ◽  
E Coli ◽  
Specific Modification ◽  
Polymerase I

Engineering large fragment insertions into the chromosome of Escherichia coli

Gene ◽  
2004 ◽  
Vol 336 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Rui Rong ◽  
Malgorzata M Slupska ◽  
Ju-Huei Chiang ◽  
Jeffrey H Miller
Keyword(s):  
Escherichia Coli ◽  
Large Fragment

Ramsey-like cardinals

2011 ◽  
Vol 76 (2) ◽  
pp. 519-540 ◽  
Author(s):  
Victoria Gitman
Keyword(s):  
Large Cardinals ◽  
Large Cardinal ◽  
Elementary Embedding ◽  
Large Fragment ◽  
Weakly Compact ◽  
Elementary Embeddings ◽  
Measurable Cardinals

AbstractOne of the numerous characterizations of a Ramsey cardinal κ involves the existence of certain types of elementary embeddings for transitive sets of size κ satisfying a large fragment of ZFC. We introduce new large cardinal axioms generalizing the Ramsey elementary embeddings characterization and show that they form a natural hierarchy between weakly compact cardinals and measurable cardinals. These new axioms serve to further our knowledge about the elementary embedding properties of smaller large cardinals, in particular those still consistent with V = L.

CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae

2018 ◽  
Vol 58 (12) ◽  
pp. 1100-1104 ◽  
Author(s):  
Zhen-Hai Li ◽  
Min Liu ◽  
Xiao-Mei Lyu ◽  
Feng-Qing Wang ◽  
Dong-Zhi Wei
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Large Fragment

Postpartum ovarian vein thrombosis

2020 ◽  
Vol 19 (5) ◽  
pp. 147-149
Author(s):  
O.V. Papysheva ◽  
◽  
A.E. Voynovskiy ◽  
A.I. Davydov ◽  
M.B. Tairova ◽  
...  
Keyword(s):  
Postpartum Period ◽  
Clinical Case ◽  
Inferior Vena ◽  
Vena Cava ◽  
Ovarian Vein ◽  
Large Fragment ◽  
Renal Veins ◽  
Vein Thrombosis ◽  
The Right ◽  
Ovarian Vein Thrombosis

A clinical case of thrombosis of the right ovarian vein in the postpartum period - an extremely rare pathology in which acute appendicitis is often suspected due to the identity of the symptoms of the diseases. Performed laparoscopy, during which a large fragment of a thrombosed vein was excised. Conclusion. Extensive right ovarian vein thrombosis with a normal hemostasiogram could not only not be resolved by anticoagulants, but also potentially spread to the renal veins and inferior vena cava, increasing the risk of pulmonary embolism. Key words: vein dissection, laparoscopy, postpartum period, ovarian vein thrombosis

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