Inhibition of EZH2 Ameliorates Cartilage Endplate Degeneration and Attenuates the Progression of Intervertebral Disc Degeneration via Demethylation of Sox-9

2019 ◽  
Author(s):  
Chao Jiang ◽  
Qiang Guo ◽  
Yu Jin ◽  
Jia-Jing Xu ◽  
Ze-Ming Sun ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Cartilage Endplate

Study of the Feasibility of Conventional MR Images and Magnetic Resonance (MR)/Ultra-Short Echo Time (UTE) Technique in the Evaluation of Lumbar Disc Degeneration in the Axial Plane

2021 ◽  
Vol 11 (3) ◽  
pp. 817-821
Author(s):  
Peng Tang ◽  
Jingtao Xu ◽  
Wei Liu ◽  
Yu Li ◽  
Wei Fan ◽  
...  
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Structural Integrity ◽  
Low Back ◽  
Pain Group ◽  
Cartilage Endplate ◽  
Significant Difference

The advantage and value of magnetic resonance (MR)-ultra-short echo time (UTE) technique in the displaying the structures of degenerative intervertebral discs were observed through evaluating the lumbar disc degeneration in the axial plane by conventional MR image and MR-UTE technique. A total of 160 examinees screened by inclusion and exclusion criteria were enrolled, and a total of 800 intervertebral disks were involved, and the degree of intervertebral disc degeneration was classified by conventional MR image classification method. After the first echo image of MR-UTE technique was integrated with the contour, the image entered UTE cartilage endplate, fibrous ring and osseous endplate. The integrity of the intervertebral disc from the above and below the intervertebral disc and the correlation of the structural endplate with the integrities of cartilage endplate, fibrous ring and osseous endplate under double echo sequence were analyzed, and the correlation analysis of the two categorical variables was conducted using rank correlation analysis. Under the MR-UTE sequence, there was no statistical significant difference in structural integrity constituent ratio (intact structure only on one side, incomplete structure on both side) between the non-low back pain group and the low back pain group (P > 0.05), the proportion of complete structure disappearance was lowest, and the proportion of partial structure disappearance was highest; there was a significant difference in the structural integrity of fibrous ring between the non-low back pain group and the low back pain group (P < 0.05), there was a statistical significant difference in structural integrity of fibrous rings between the two groups (P < 0.05); there was a statistical significant difference in pain degree between the partial structure disappearance group and the complete structure disappearance group (P < 0.05). There was no significant difference in pain degree between the groups (intact structure only on one side and incomplete structure on both side); conventional MR images showed no significant difference in the intervertebral disc degeneration degree between the non-low back pain group and low back pain group (P > 0.05), and the intervertebral disc degeneration degree had no significant correlation with the lower back pain. MR-UTE sequence and T2WI sequence showed that there was a correlation between different intervertebral disc degeneration degrees and the structural integrity distributions of cartilage endplate, osseous endplate and fibrous rings in two groups of patients with or without low back pain (P < 0.05). Whether low back pain exists or not has little effect on the intervertebral disc degeneration degree on T2WI. MR-UTE double echo sequence scan reveals that the intervertebral disc degeneration degree is positively related to the integrity and integrity distribution of cartilage endplate, fibrous ring and bone endplate.

scholarly journals Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

2022 ◽  
Vol 9 ◽  
Author(s):  
Weishi Liang ◽  
Bo Han ◽  
Yong Hai ◽  
Duan Sun ◽  
Peng Yin
Keyword(s):  
Stem Cells ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Bone Repair ◽  
Fine Tuning ◽  
Regulatory Function ◽  
Cartilage Endplate ◽  
Bone Injury ◽  
And Cartilage

Exosomes are extracellular vesicles formed by various donor cells that regulate gene expression and cellular function in recipient cells. Exosomes derived from mesenchymal stem cells (MSC-Exos) perform the regulatory function of stem cells by transporting proteins, nucleic acids, and lipids. Intervertebral disc degeneration (IDD) is one of the main causes of low back pain, and it is characterized by a decreased number of nucleus pulposus cells, extracellular matrix decomposition, aging of the annulus fibrosus, and cartilage endplate calcification. Besides, nutrient transport and structural repair of intervertebral discs depend on bone and cartilage and are closely related to the state of the bone. Trauma, disease and aging can all cause bone injury. However, there is a lack of effective drugs against IDD and bone injury. Recent MSC-Exos fine tuning has led to significant progress in the IDD treatment and bone repair and regeneration. In this review, we looked at the uniqueness of MSC-Exos, and the potential treatment mechanisms of MSC-Exos with respect to IDD, bone defects and injuries.

scholarly journals Effects of Endplate Healing Morphology on Intervertebral Disc Degeneration after Pedicle Screw Fixation for Thoracolumbar Fractures

2020 ◽  
Author(s):  
Yunshan Su ◽  
Dong Ren ◽  
Dehang Liu ◽  
Junfei Li ◽  
Tianci Wang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Pedicle Screw ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Clinical Decision Making ◽  
Screw Fixation ◽  
Pedicle Screw Fixation ◽  
Thoracolumbar Fractures ◽  
Cartilage Endplate

Abstract Background: The cartilage endplate plays an important role in the stress distribution and nutrition metabolism of intervertebral disc. The healing morphology of the endplate after spinal fracture and its effect on the intervertebral disc degeneration are still unclear. Methods: 51 patients with single-level thoracolumbar fractures underwent posterior open reduction and pedicle screw fixation. Combined with the initial CT and MRI images, the endplate injury was defined as three types, which are unilateral endplate injury, bilateral endplate injury and no endplate injury. According to the location of the injury in the endplate, it was further divided into endplate central injury and endplate peripheral injury. The degree of post-traumatic disc lesions and disc degeneration during follow-up were classified based on the Sander classification and the Pfirrmann classification, respectively. According to the T1 image of MRI in the final follow-up, the healing morphology of endplates was classified into three types including the increased endplate curvature, the irregular healing and the traumatic Schmorl nodes. Results: Cartilage endplate fractures are closely related to the degree of degeneration of the intervertebral disc (P=0.003). Injuries in different parts of the endplate have no significant effect on the intervertebral disc degeneration (P=0.204). The healing morphology after endplate fracture significantly affected the degree of intervertebral disc degeneration (P=0.001). The comparison of groups showed that the effects of irregular healing and traumatic Schmorl nodes on disc degeneration were not statistically different, but were significantly different from those with increased curvature. Conclusions: Increased endplate curvature, irregular healing and traumatic Schmorl nodes are three common forms of healing. The irregular healing and the traumatic Schmorl nodes are closely related to intervertebral disc degeneration. The existence and severity of the endplate injury can provide valuable information for individualized clinical decision-making processes.

scholarly journals miR-142-3p and HMGB1 Are Negatively Regulated in Proliferation, Apoptosis, Migration and Autophagy of Cartilage Endplate Cells

2021 ◽  
Author(s):  
Bo Wang ◽  
Demin Ji ◽  
Wenhua Xing ◽  
Feng Li ◽  
Zhi Huang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Target Gene ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Vital Role ◽  
Luciferase Assay ◽  
Expression Levels ◽  
Cartilage Endplate

Abstract Background: cartilage endplate (CEP) degeneration plays a vital role in the pathological process of intervertebral disc degeneration. It has been previously reported that microRNAs may participate in the occurrence and development of intervertebral disc degeneration through regulating its’ target genes directly. The regulatory roles of miR-142-3p /HMGB1 in some orthopedic diseases have been determined successively, but there was no report about the degeneration of CEP. Therefore, we aimed to determine the regulation of miR-142-3p /HMGB1or potential molecular mechanisms on proliferation, apoptosis, migration, and autophagy of CEP cells.Methods: the target gene of miR-142-3p was determined by double luciferase assay. We selected ATDC5 cell lines. CCK-8 method was used to detect cell proliferation. Real-time fluorescence quantitative PCR was used to determine gene expression levels, and western blot analysis was used to determine protein expression levels. We chose the flow cytometry to measure cell apoptosis and cell cycle.Results: the result of luciferase detection showed that the target gene of miR-142-3p in CEP cells was HMGB1. Knockdown the miR-142-3p inhibited the expression level of HMGB1, the proliferation and migration of CEP cells, but it promoted apoptosis of CEP cells. In addition, the detection results of the proteins related to apoptosis or autophagy showed that knockdown of miR-142-3p promoted apoptosis and autophagy.Conclusion: The negative regulation of miR-142-3p /HMGB1 can affect the proliferation, apoptosis, migration, and autophagy of CEP cells. Our results provided a new idea for the targeted treatment of CEP degeneration by inhibiting the expression of HMGB1.

scholarly journals Evaluation of Cartilaginous Endplate Degeneration Based on Magnetic Resonance Imaging

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Xiaofeng Chen ◽  
Weijun Guo ◽  
Hao Li ◽  
Xi Li ◽  
Zhuangxun Han ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Resonance Imaging ◽  
Lumbar Intervertebral Disc ◽  
Cartilage Endplate ◽  
Study Results ◽  
Cartilaginous Endplate

In order to carry out the evaluation of cartilaginous endplate degeneration based on magnetic resonance imaging (MRI), this paper retrospectively analyzed the MRI data from 120 cases of patients who were diagnosed as lumbar intervertebral disc degeneration and underwent MRI examinations in the designated hospital of this study from June 2018 to June 2020. All cases underwent conventional sagittal and transverse T1WI and T2WI scans, and some cases were added with sagittal fat-suppression T2WI scans; then, the number of degenerative cartilaginous endplates and its ratio to degenerative lumbar intervertebral discs were counted and calculated, and the T1WI and T2WI signal characteristics of each degenerative cartilage endplate and its correlation with cartilaginous endplate degeneration were summarized, compared, and analyzed to evaluate the cartilaginous endplate degeneration by those magnetic resonance information. The study results show that there were 33 cases of cartilaginous endplate degeneration, accounting for 27.50% of all those 120 patients with lumbar intervertebral disc degeneration (54 degenerative endplates in total), including 9 cases with low T1WI and high T2WI signals, 5 cases with high T1WI and low T2WI signals, 12 cases with high and low mixed T1WI and high or mixed T2WI signals, and 4 cases with both low T1WI and T2WI signals. Therefore, MRI scanning can clearly present the abnormal signals of lumbar intervertebral disc and cartilaginous endplate degeneration, accurately identity their lesion locations, and type their degenerative characteristics, which may be best inspection method for the evaluation of cartilaginous endplate degeneration in the early diagnosis of intervertebral disc degeneration. The study results of this paper provide a reference for further researches on the evaluation of cartilaginous endplate degeneration based on magnetic resonance imaging.

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