Metabolic Plasticity of IDH1- Mutant Glioma Cell Lines Is Responsible for Low Sensitivity to Glutaminase Inhibition

2019 ◽  
Author(s):  
Victor Ruiz-Rodado ◽  
Adrian Lita ◽  
Tyrone Dowdy ◽  
Orieta Celiku ◽  
Alejandra Cavazos-Saldana ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Metabolic Plasticity ◽  
Glioma Cell Lines ◽  
Low Sensitivity

scholarly journals Metabolic plasticity of IDH1-mutant glioma cell lines is responsible for low sensitivity to glutaminase inhibition

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Victor Ruiz-Rodado ◽  
Adrian Lita ◽  
Tyrone Dowdy ◽  
Orieta Celiku ◽  
Alejandra Cavazos Saldana ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Tca Cycle ◽  
Glutamine Metabolism ◽  
Loss Of Function ◽  
Metabolic Plasticity ◽  
Glioma Cell Lines ◽  
Low Sensitivity

Abstract Background Targeting glutamine metabolism in cancer has become an increasingly vibrant area of research. Mutant IDH1 (IDH1mut) gliomas are considered good candidates for targeting this pathway because of the contribution of glutamine to their newly acquired function: synthesis of 2-hydroxyglutarate (2HG). Methods We have employed a combination of 13C tracers including glutamine and glucose for investigating the metabolism of patient-derived IDH1mut glioma cell lines through NMR and LC/MS. Additionally, genetic loss-of-function (in vitro and in vivo) approaches were performed to unravel the adaptability of these cell lines to the inhibition of glutaminase activity. Results We report the adaptability of IDH1mut cells’ metabolism to the inhibition of glutamine/glutamate pathway. The glutaminase inhibitor CB839 generated a decrease in the production of the downstream metabolites of glutamate, including those involved in the TCA cycle and 2HG. However, this effect on metabolism was not extended to viability; rather, our patient-derived IDH1mut cell lines display a metabolic plasticity that allows them to overcome glutaminase inhibition. Conclusions Major metabolic adaptations involved pathways that can generate glutamate by using alternative substrates from glutamine, such as alanine or aspartate. Indeed, asparagine synthetase was upregulated both in vivo and in vitro revealing a new potential therapeutic target for a combinatory approach with CB839 against IDH1mut gliomas.

Human Malignant Glioma Cell Lines are Refractory to Retinoic Acid-Mediated Differentiation and Sensitization to Apoptosis1

2000 ◽  
Vol 10 (3) ◽  
pp. 159-168 ◽  
Author(s):  
Friederike Schmidt ◽  
Peter Groscurth ◽  
Johannes Dichgans ◽  
Michael Weller
Keyword(s):  
Retinoic Acid ◽  
Malignant Glioma ◽  
Cell Lines ◽  
Glioma Cell ◽  
Glioma Cell Lines ◽  
Human Malignant Glioma

Anti-oxidant and anti-proliferative effect of anthocyanin enriched fractions from two Mexican wild blackberries (Rubus spp.) on HepG2 and glioma cell lines

2020 ◽  
pp. 1-17
Author(s):  
Oscar Abel Sánchez-Velázquez ◽  
Mireli Cortés-Rodríguez ◽  
Jorge Milán-Carrillo ◽  
Julio Montes-Ávila ◽  
Benjamín Robles-Bañuelos ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Proliferative Effect ◽  
Glioma Cell Lines ◽  
Anti Oxidant

scholarly journals Estradiol Receptors Regulate Differential Connexin 43 Expression in F98 and C6 Glioma Cell Lines

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0150007 ◽  
Author(s):  
Zahra Moinfar ◽  
Hannes Dambach ◽  
Bodo Schoenebeck ◽  
Eckart Förster ◽  
Nora Prochnow ◽  
...  
Keyword(s):  
Cell Lines ◽  
Connexin 43 ◽  
Glioma Cell ◽  
C6 Glioma ◽  
C6 Glioma Cell ◽  
Estradiol Receptors ◽  
Glioma Cell Lines

Enhancement of temozolomide-induced apoptosis by valproic acid in human glioma cell lines through redox regulation

2011 ◽  
Vol 89 (3) ◽  
pp. 303-315 ◽  
Author(s):  
Ching-Hsein Chen ◽  
Yu-Jia Chang ◽  
Maurice S. B. Ku ◽  
King-Thom Chung ◽  
Jen-Tsung Yang
Keyword(s):  
Valproic Acid ◽  
Cell Lines ◽  
Glioma Cell ◽  
Redox Regulation ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Glioma Cell Lines ◽  
Induced Apoptosis

Pharmacogenomic Approach Reveals a Role for the xc− Cystine/Glutamate Antiporter in Growth and Celastrol Resistance of Glioma Cell Lines

2009 ◽  
Vol 332 (3) ◽  
pp. 949-958 ◽  
Author(s):  
Anh-Nhan Pham ◽  
Paul E. Blower ◽  
Omar Alvarado ◽  
Ranadheer Ravula ◽  
Peter W. Gout ◽  
...  
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Glioma Cell Lines

Cytotoxicity of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles in rat glioma cell lines using different assays

2006 ◽  
Vol 14 (9) ◽  
pp. 614-622 ◽  
Author(s):  
Berta Sanchez De Juan ◽  
Hagen Von Briesen ◽  
Svetlana E. Gelperina ◽  
Jörg Kreuter
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Rat Glioma ◽  
Glioma Cell Lines

Identity confusion of glioma cell lines

Gene ◽  
2014 ◽  
Vol 536 (1) ◽  
pp. 221-222 ◽  
Author(s):  
Dmitriy Timerman ◽  
Caleb M. Yeung
Keyword(s):  
Cell Lines ◽  
Glioma Cell ◽  
Identity Confusion ◽  
Glioma Cell Lines
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