The Mechanism of Mica on Protecting NSAIDs Induced Small Intestinal Mucosal Barrier Injury via ncRuPAR-PAR2 Pathway

2019 ◽  
Author(s):  
Guanqun Chao ◽  
Fangxu Ye ◽  
Shuo Zhang
Keyword(s):  
Mucosal Barrier ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury ◽  
Small Intestinal

scholarly journals Dietary l-Tryptophan Supplementation Enhances the Intestinal Mucosal Barrier Function in Weaned Piglets: Implication of Tryptophan-Metabolizing Microbiota

2018 ◽  
Vol 20 (1) ◽  
pp. 20 ◽  
Author(s):  
Haiwei Liang ◽  
Zhaolai Dai ◽  
Jiao Kou ◽  
Kaiji Sun ◽  
Jingqing Chen ◽  
...  
Keyword(s):  
Tight Junction ◽  
Mucosal Barrier ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Tight Junction Proteins ◽  
Weaned Piglets ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Function ◽  
Small Intestinal ◽  
Junction Proteins

l-Tryptophan (Trp) is known to play an important role in the health of the large intestine. However, a role of dietary Trp in the small-intestinal mucosal barrier and microbiota remains poorly understood. The present study was conducted with weaned piglets to address this issue. Postweaning piglets were fed for 4 weeks a corn- and soybean meal-based diet supplemented with 0 (Control), 0.1, 0.2, or 0.4% Trp. The small-intestinal microbiota and serum amino acids were analyzed by bacterial 16S rRNA gene-based high-throughput sequencing methods and high-performance liquid chromatography, respectively. The mRNA levels for genes involved in host defense and the abundances of tight-junction proteins in jejunum and duodenum were measured by real time-PCR and Western blot techniques, respectively. The concentrations of Trp in the serum of Trp-supplemented piglets increased in a dose-dependent manner. Compared with the control group, dietary supplementation with 0.2–0.4% Trp reduced the abundances of Clostridium sensu stricto and Streptococcus in the jejunum, increased the abundances of Lactobacillus and Clostridium XI (two species of bacteria that can metabolize Trp) in the jejunum, and augmented the concentrations of secretory immunoglobulin A (sIgA) as well as mRNA levels for porcine β-defensins 2 and 3 in jejunal tissues. Moreover, dietary Trp supplementation activated the mammalian target of rapamycin signaling and increased the abundances of tight-junction proteins (zonula occludens (ZO)-1, ZO-3, and claudin-1) in jejunum and duodenum. We suggested that Trp-metabolizing bacteria in the small intestine of weaned pigs primarily mediated the beneficial effects of dietary Trp on its mucosal integrity, health, and function.

scholarly journals Prospective study on the effect of abdominal hypertension on intestinal mucosal barrier injury during laparoscopic surgery

2019 ◽  
Author(s):  
Yong Yang ◽  
Xin kang ◽  
Xingjian Yang ◽  
Yi Hu ◽  
Rong Chen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Diamine Oxidase ◽  
Measurement Data ◽  
Postoperative Recovery ◽  
Mucosal Barrier ◽  
Co2 Pneumoperitoneum ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury ◽  
Abdominal Hypertension ◽  
Group 1

Abstract Objective Prolonged and high intraperitoneal pressure may lead to impaired intestinal mucosal blood perfusion, increase the risk of surgery and complications, and affect the postoperative recovery of patients. However, the literature reports on the effect of abdominal hypertension on gastrointestinal function mainly focus on animal experiments, and there are few clinical reports. Our study intends to explore the effect of increased CO2 pneumoperitoneum pressure during laparoscopy on intestinal mucosal barrier injury. Methods A prospective study was conducted on 180 patients who underwent laparoscopic cholecystectomy in the First People's Hospital of Shuangliu District, Chengdu from October 2017 to March 2018. A randomized，single-blind，controlled study was performed in the 180 patients who were allocated into the 10 mmHg group(1 mmHg=0.133 kPa)，12 mmHg group and 15 mmHg group based on a random number table and setting value of intraoperative CO2 pneumoperitoneum pressure (10 mmHg，12 mmHg and 15 mmHg)．Main observation indexes such as intraoperative conditions and postoperative recovery were recorded, and the results of serum tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), D-lactic acid, blood endotoxin levels，plasma diamine oxidase (DAO) activity were detected. The measurement data of normal distribution were expressed as mean ± standard deviation (x+S), and one-way analysis of variance was used for comparison between groups. The measurement data with non-normal distribution are represented by M (QR) and non-parametric test is adopted, count data were presented as the n(%), and comparison among groups was analyzed using the chi-square test. Results 180 patients were screened out, 60 patients in each group. Eight patients dropped out during the study (2 in 10 mmHg group, 1 in 12 mmHg group and 5 in 15 mmHg group). All patients in the three groups were cured and discharged without bleeding, secondary bile duct stones, bile leakage and reoperation. There was no significant difference in serum TNF-a, IL-1, D-lactic acid, endotoxin level and plasma diamine oxidase (DAO) among the three groups after operation (P>0.05).Conclusion Laparoscopic surgery under 15 mmHg CO2 pneumoperitoneum did not cause intestinal mucosal barrier damage, and the operation under the pneumoperitoneum was safe and reliable.Registry number of ChiCTR1900023936.

BRCC36 promotes intestinal mucosal barrier injury caused by BMP2 after ischemia-reperfusion via inhibiting PPARγ signaling

2021 ◽  
Author(s):  
Jin-Ming Zhang ◽  
Kun-Nan Wang ◽  
Yun Zhang ◽  
Jun-Ze Zhang ◽  
Xin-Pu Yuan ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Bone Morphogenetic Protein 2 ◽  
Mucosal Barrier ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Intestinal Mucosal Barrier ◽  
Morphogenetic Protein ◽  
Mucosal Barrier Injury ◽  
Brca1 Brca2 ◽  
Intestinal Ischemia Reperfusion

Abstract As one of the most common pathological changes in trauma and surgery practice, intestinal ischemia-reperfusion (I/R) injury is regarded as a major precipitating factor in the occurrence and development of fatal diseases. BRCA1-BRCA2-containing complex subunit 36 (BRCC36), a deubiquitinase, has been proved important in a variety of pathophysiological processes such as DNA repair, cell cycle regulation, tumorigenesis and inflammatory response. However, the effect of BRCC36 on intestinal mucosal barrier injury after I/R has not been fully elucidated. Our research found that BRCC36 aggravated intestinal mucosal barrier injury caused by BMP2 (Bone morphogenetic protein 2) after I/R by downregulating PPARγ (Peroxisome proliferator-activated receptor-γ) signaling. These results suggested that BRCC36/PPARγ axis might serve as a potential therapeutic target for preventing intestinal mucosal barrier injury after I/R.

Leptin alleviates intestinal mucosal barrier injury and inflammation in obese mice with acute pancreatitis

2018 ◽  
Vol 42 (8) ◽  
pp. 1471-1479 ◽  
Author(s):  
Cheng Ye ◽  
Rui Wang ◽  
Mojin Wang ◽  
Zhiyin Huang ◽  
Chengwei Tang
Keyword(s):  
Acute Pancreatitis ◽  
Mucosal Barrier ◽  
Obese Mice ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury

Fecal Microbiota Transplantation Repairs Intestinal Mucosal Barrier Injury in Mice with Ulcerative Colitis

2021 ◽  
Vol 15 (5) ◽  
pp. 679-684
Author(s):  
Yijuan Lin ◽  
Jian Ding ◽  
Xunru Huang ◽  
Jintong Chen ◽  
Chengdang Wang
Keyword(s):  
Structural Changes ◽  
Fecal Microbiota Transplantation ◽  
Intestinal Barrier ◽  
Fecal Microbiota ◽  
Mucosal Barrier ◽  
Control Group ◽  
Model Group ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury ◽  
Group A

This study aimed to explore the effects of fecal microbiota transplantation (FMT) on intestinal mucosal barrier injury in mice with ulcerative colitis (UC) and to elucidate the underlying mechanisms. Dextran sodium sulfate (DSS) was administered to develop the UC mouse model. Next, the experiment was divided into a normal control group, a DSS model group, a DSS+5-amino acid salicylic acid (5-ASA) group, and a DSS+FMT group. Hematoxylin–eosin staining was used to detect pathological changes; transmission electron microscopy was used to evaluate structural changes of intestinal mucosa; enzyme-linked immunosorbent assay (ELSIA) was used to detect endotoxins; and western blotting was used to detect the expression of zonula occludens-1 (ZO-1). In the control group, the intestinal mucosa and microvilli were intact, epithelial cells were closely connected, and the intercellular space was narrow. By contrast, focal intestinal barrier defects, including shallow ulcer, local inflammatory cell infiltration, hyperplasia of connective tissue, and loss of gland structure were observed in the model group. These abnormal morphological and structural changes were ameliorated by 5-ASA and FMT. Compared with the control group, the endotoxin content increased significantly, and the ZO-1 protein expression decreased significantly in the model group (P < 0.05). By contrast, the endotoxin level decreased significantly, and the ZO-1 protein expression increased significantly in the 5-ASA group and FMT group compared with that of the model group (P < 0.05). FMT ameliorates UC by repairing the intestinal barrier function, which is likely involved in upregulating ZO-1 expression.

scholarly journals Prospective study on the effect of abdominal hypertension on intestinal mucosal barrier injury during laparoscopic surgery

2020 ◽  
Author(s):  
yong yang ◽  
Xin kang ◽  
Xingjian Yang ◽  
Yi Hu ◽  
Rong Chen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Laparoscopic Surgery ◽  
Diamine Oxidase ◽  
Measurement Data ◽  
Postoperative Recovery ◽  
Mucosal Barrier ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury ◽  
Abdominal Hypertension ◽  
Group 1

Abstract Background Prolonged and high intraperitoneal pressure may lead to impaired intestinal mucosal blood perfusion, increase the risk of surgery and complications, and affect the postoperative recovery of patients. However, the literature reports on the effect of abdominal hypertension on gastrointestinal function mainly focus on animal experiments, and there are few clinical reports. Our study intends to explore the effect of increased CO2 pneumoperitoneum pressure during laparoscopy on intestinal mucosal barrier injury. Methods A prospective study was conducted on 180 patients who underwent laparoscopic cholecystectomy in the First People's Hospital of Shuangliu District, Chengdu from October 2017 to March 2018. A randomized，single-blind，controlled study was performed in the 180 patients who were allocated into the 10 mmHg group(1 mmHg=0.133 kPa)，12 mmHg group and 15 mmHg group based on a random number table and setting value of intraoperative CO2 pneumoperitoneum pressure (10 mmHg，12 mmHg and 15 mmHg)．Main observation indexes such as intraoperative conditions and postoperative recovery were recorded, and the results of serum tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), D-lactic acid, blood endotoxin levels，plasma diamine oxidase (DAO) activity were detected. The measurement data of normal distribution were expressed as mean ± standard deviation (`x+S), and one-way analysis of variance was used for comparison between groups. The measurement data with non-normal distribution are represented by M (QR) and non-parametric test is adopted, count data were presented as the n(%), and comparison among groups was analyzed using the chi-square test. Results 180 patients were screened out, 60 patients in each group. Eight patients dropped out during the study (2 in 10 mmHg group, 1 in 12 mmHg group and 5 in 15 mmHg group). All patients in the three groups were cured and discharged without bleeding, secondary bile duct stones, bile leakage and reoperation. There was no significant difference in serum TNF-a, IL-1, D-lactic acid, endotoxin level and plasma diamine oxidase (DAO) among the three groups after operation (P>0.05).Conclusion Laparoscopic surgery under 15 mmHg CO2 pneumoperitoneum did not cause intestinal mucosal barrier damage, and the operation under the pneumoperitoneum was safe and reliable. Registry：This study was registered with the Chinese Clinical Trial Registry with the registry number of ChiCTR1900023936.Registered 19 June ，2019（retrospectively registered）.

Sign in / Sign up

Export Citation Format

Share Document