Investigation of Tetrahydroxystilbene Glucoside, the Idiosyncratic Hepatotoxic Component of Polygonum multiflorum Thunb., and Its Toxicity Attenuation by Ganoderma lucidum

2019 ◽  
Author(s):  
Yan Lin ◽  
Xi Peng ◽  
Rong Xiao ◽  
Bo Hou Xia ◽  
Zhi Min Zhang ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Polygonum Multiflorum ◽  
Tetrahydroxystilbene Glucoside

Tetrahydroxystilbene glucoside alleviates Ang Ⅱ-induced senescence of HUVECs via SIRT1

2020 ◽  
Author(s):  
Wenxue Fan ◽  
Yan Guo ◽  
Shidong Cao ◽  
Shuyu Cao ◽  
Yuefeng Xie ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Senescent Cell ◽  
Expression Level ◽  
Protective Mechanism ◽  
Aging Effects ◽  
Polygonum Multiflorum ◽  
Upward Trend ◽  
Umbilical Vein Endothelial Cells ◽  
Tetrahydroxystilbene Glucoside ◽  
Pai 1

Tetrahydroxystilbene glucoside (TSG), an active ingredient of <i>Polygonum multiflorum</i>, has been known for certain anti-aging effects. In this study, the possible protective mechanism of TSG on human umbilical vein endothelial cells (HUVECs) senescence induced by angiotensin Ⅱ (Ang Ⅱ) was investigated. The results revealed that TSG pretreatment could reduce the percentage of senescence-associated-β-galactosidase (SA-β-gal) positive cells, and decrease the expression levels of the cellular senescence biomarkers, p53 and PAI-1 proteins. At the same time, the expression of SIRT1 in senescent cell showed an upward trend due to TSG treatment. When inhibiting the expression of SIRT1 by EX527, our results showed that TSG reversed the effect of EX527, by promoting the expression level of SIRT1, reducing the expression of SA-β-gal positive cell and the expression level of p53 and PAI-1 proteins. The present study demonstrated that TSG could protect against HUVECs senescence induced by Ang Ⅱ, potentially through modulation of SIRT1 activity.

scholarly journals HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Weihua Yu ◽  
Xiaodi Zhang ◽  
Hao Wu ◽  
Qingbiao Zhou ◽  
Zhao Wang ◽  
...  
Keyword(s):  
Mitochondrial Biogenesis ◽  
Mammalian Cells ◽  
Inflammatory Diseases ◽  
Heme Oxygenase 1 ◽  
Zinc Protoporphyrin ◽  
Polygonum Multiflorum ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
And Function ◽  
Tetrahydroxystilbene Glucoside

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an important monomer extracted from Polygonum multiflorum, can prevent a number of inflammation associated chronic diseases. However, the mechanism involved in TSG inducing anti-inflammatory role remains unclear. As an inducible antioxidant enzyme, Heme oxygenase-1 (HO-1), is crucial for protecting the mammalian cells against adverse stimuli. Here, we found that the TSG treatment strongly induces the expression of HO-1 in an NRF2-depended manner. Meanwhile, TSG increased the mitochondrial mass through upregulation of the mitochondrial biogenesis activators (PGC-1α, NRF1, and TFAM) as well as the mitochondrial complex IV. Furthermore, TSG attenuated Lipopolysaccharide (LPS) mediated RAW264.7 cells activation and secretion of proinflammatory cytokines, including interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Zinc Protoporphyrin (ZnPP), a selective inhibitor of HO-1 activity, was able to attenuate TSG mediated mitochondrial biogenesis and anti-inflammatory process. Finally, we observed that LPS induced obvious mtDNA depletion and ATP deficiency, which indicated a severe damage of mitochondria. TSG restored the LPS induced mitochondrial dysfunction via activation of the mitochondrial biogenesis. ZnPP treatment markedly reversed the inhibitory effects of TSG on mitochondrial damage and oxidative stress in LPS stimulated macrophages. Taken together, these findings suggest that TSG enhances mitochondrial biogenesis and function mainly via activation the HO-1. TSG can be developed as a potential drug for treatment of inflammatory diseases.

scholarly journals Tetrahydroxystilbene Glucoside Produces Neuroprotection against 6-OHDA-Induced Dopamine Neurotoxicity

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Chun Huang ◽  
Fengqin Lin ◽  
Guoqing Wang ◽  
Disheng Lu ◽  
Qin Wu ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Mapk Signaling ◽  
Microglia Activation ◽  
Mitogen Activated Protein Kinase ◽  
Therapeutic Effects ◽  
Polygonum Multiflorum ◽  
Underlying Mechanisms ◽  
6 Hydroxydopamine ◽  
Tetrahydroxystilbene Glucoside

Parkinson’s disease (PD) was one of the most common neurodegenerative diseases with a slow and progressive loss of dopamine (DA) neurons in the midbrain substantia nigra (SN). Neuroinflammation was identified to be an important contributor to PD pathogenesis with the hallmark of microglia activation. Tetrahydroxystilbene glucoside (TSG) was the main active component extracted from Polygonum multiflorum and held amounts of pharmacological activities including antioxidant, free radical-scavenging, anti-inflammation, and cardioprotective properties. Recent studies demonstrated that TSG exerted neuroprotection from several neurodegenerative disease models. However, the underlying mechanisms were not completely elucidated. In the present study, rat nigral stereotaxic injection of 6-hydroxydopamine- (6-OHDA-) elicited DA neuronal injury was performed to investigate TSG-mediated neuroprotection on DA neurons. In addition, primary rat midbrain neuron-glia cocultures were applied to explore the mechanisms underlying TSG-exerted neuroprotection. Results showed that daily intraperitoneal injection of TSG for 14 consecutive days significantly protected DA neurons from 6-OHDA-induced neurotoxicity and suppressed microglia activation. Similar neuroprotection was shown in primary neuron-glia cocultures. In vitro studies further demonstrated that TSG inhibited microglia activation and subsequent release of proinflammatory factors. Moreover, TSG-mediated neuroprotection was closely related with the inactivation of mitogen-activated protein kinase (MAPK) signaling pathway. Together, TSG protects DA neurons from 6-OHDA-induced neurotoxicity via the inhibition of microglia-elicited neuroinflammation. These findings suggest that TSG might hold potential therapeutic effects on PD.

scholarly journals Tetrahydroxystilbene Glucoside Effectively Prevents Apoptosis Induced Hair Loss

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Lulu Chen ◽  
Huichuan Duan ◽  
Feng Xie ◽  
Zhen Gao ◽  
Xiaoli Wu ◽  
...  
Keyword(s):  
Hair Loss ◽  
Caspase 3 ◽  
Polygonum Multiflorum ◽  
Caspase 9 ◽  
Hair Regrowth ◽  
Growth Promoting ◽  
Induced Apoptosis ◽  
Main Component ◽  
Tetrahydroxystilbene Glucoside ◽  
Active Caspase

The effect of Polygonum multiflorum against hair loss has been widely recognized. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is the main component of Polygonum multiflorum; however, its role in hair regeneration has not been established. To evaluate the hair growth-promoting activity of TSG, depilated C57BL/6J mice were topically treated with normal saline, TSG, Pifithrin-α, Minoxidil for 2 weeks. In this study, we identified that p53, Caspase-3, Active Caspase-3, and Caspase-9 were obviously upregulated in the skin of human and mice with hair loss by western blot analysis. Depilated mice treated with TSG showed markedly hair regrowth. TUNEL+ cells were also reduced in mice with TSG. These changes were accompanied with inhibition of Fas, p53, Bax, Active Caspase-3, and Procaspase-9 activities. These results demonstrated that TSG exerts great hair regrowth effect on hair loss, which was probably mediated by inhibition of p53, Fas, and Bax induced apoptosis.

Artificial Hybridization of Ganoderma lucidum and G. tsugae Murrill by Protoplast Fusion for Sustainability

2005 ◽  
Vol 7 (1-2) ◽  
pp. 263-280 ◽  
Author(s):  
Siu Wai Chiu ◽  
Vivien Wing Yan Luk ◽  
Stephen Yu ◽  
Peggy Lee ◽  
Natalie Wai ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Ganoderma Lucidum ◽  
Artificial Hybridization
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