Necroptosis of Intestinal Epithelial Cells Induces Type 3 Innate Lymphoid Cell-Dependent Lethal Ileitis

2019 ◽  
Author(s):  
Ryodai Shindo ◽  
Masaki Ohmuraya ◽  
Sachiko Komazawa-Sakon ◽  
Sanae Miyake ◽  
Soh Yamazaki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lymphoid Cell ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Innate Lymphoid Cell ◽  
Type 3

scholarly journals Innate lymphoid cell type 3–derived interleukin-22 boosts lipocalin-2 production in intestinal epithelial cells via synergy between STAT3 and NF-κB

2019 ◽  
Vol 294 (15) ◽  
pp. 6027-6041 ◽  
Author(s):  
Maarten Coorens ◽  
Anna Rao ◽  
Stefanie Katharina Gräfe ◽  
Daniel Unelius ◽  
Ulrik Lindforss ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lymphoid Cell ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Lipocalin 2 ◽  
Cell Type ◽  
Innate Lymphoid Cell ◽  
Interleukin 22 ◽  
Type 3

scholarly journals Necroptosis of Intestinal Epithelial Cells Induces Type 3 Innate Lymphoid Cell-Dependent Lethal Ileitis

iScience ◽  
2019 ◽  
Vol 15 ◽  
pp. 536-551 ◽  
Author(s):  
Ryodai Shindo ◽  
Masaki Ohmuraya ◽  
Sachiko Komazawa-Sakon ◽  
Sanae Miyake ◽  
Yutaka Deguchi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lymphoid Cell ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Innate Lymphoid Cell ◽  
Type 3

Reovirus type 1 and type 3 differ in their binding to isolated intestinal epithelial cells

1988 ◽  
Vol 5 (1) ◽  
pp. 29-40 ◽  
Author(s):  
David B. Weiner ◽  
Karen Girard ◽  
William V. Williams ◽  
Thomas McPhillips ◽  
Donald H. Rubin
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Reovirus Type ◽  
Type 3

scholarly journals Transient Suppression of Shigella flexneri Type 3 Secretion by a Protective O-Antigen-Specific Monoclonal IgA

mBio ◽  
2011 ◽  
Vol 2 (3) ◽  
Author(s):  
Stephen J. Forbes ◽  
Tia Bumpus ◽  
Elizabeth A. McCarthy ◽  
Blaise Corthésy ◽  
Nicholas J. Mantis
Keyword(s):  
Epithelial Cells ◽  
Mucosal Immunity ◽  
Intestinal Epithelial Cells ◽  
Shigella Flexneri ◽  
Secretory Iga ◽  
Bacterial Invasion ◽  
Intestinal Epithelial ◽  
Content Type ◽  
O Antigen ◽  
Type 3

ABSTRACTMucosal immunity to the enteric pathogenShigella flexneriis mediated by secretory IgA (S-IgA) antibodies directed against the O-antigen (O-Ag) side chain of lipopolysaccharide. While secretory antibodies against the O-Ag are known to prevent bacterial invasion of the intestinal epithelium, the mechanisms by which this occurs are not fully understood. In this study, we report that the binding of a murine monoclonal IgA (IgAC5) to the O-Ag ofS. flexneriserotype 5a suppresses activity of the type 3 secretion (T3S) system, which is necessary forS. flexnerito gain entry into intestinal epithelial cells. IgAC5’s effects on the T3S were rapid (5 to 15 min) and were coincident with a partial reduction in the bacterial membrane potential and a decrease in intracellular ATP levels. Activity of the T3S system returned to normal levels 45 to 90 min following antibody treatment, demonstrating that IgAC5’s effects were transient. Nonetheless, these data suggest a model in which the association of IgA with the O-Ag ofS. flexneripartially de-energizes the T3S system and temporarily renders the bacterium incapable of invading intestinal epithelial cells.IMPORTANCESecretory IgA (S-IgA) serves as the first line of defense against enteric infections. However, despite its well-recognized role in mucosal immunity, relatively little is known at the molecular level about how this class of antibody functions to prevent pathogenic bacteria from penetrating the epithelial barrier. It is generally assumed that S-IgA functions primarily by “immune exclusion,” a phenomenon in which the antibody binds to microbial surface antigens and thereby promotes bacterial agglutination, entrapment in mucus, and physical clearance from the gastrointestinal tract via peristalsis. The results of the present study suggest that in addition to serving as a physical barrier, S-IgA may have a direct impact on the ability of microbial pathogens to secrete virulence factors required for invasion of intestinal epithelial cells.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

Thrombin enhances migration of intestinal epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A504-A504
Author(s):  
A NEUMANN ◽  
M DEPKAPRONDZINSKI ◽  
C WILHELM ◽  
K FELGENHAUER ◽  
T CASPRITZ ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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