RGD-Containing Elastin Like Polypeptide Improves Islet Transplantation Outcomes in Diabetic Mice Via Upregulation of Islet Survival and Vessel Formation

2019 ◽  
Author(s):  
Kyeong-Min Lee ◽  
Jung-Hee Kim ◽  
Eun-Sook Choi ◽  
Eunjoo Kim ◽  
Seong-Kyoon Choi ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Mice ◽  
Vessel Formation ◽  
Islet Survival ◽  
Transplantation Outcomes

scholarly journals RGD-containing elastin-like polypeptide improves islet transplantation outcomes in diabetic mice

2019 ◽  
Vol 94 ◽  
pp. 351-360 ◽  
Author(s):  
Kyeong-Min Lee ◽  
Jung-Hee Kim ◽  
Eun-Sook Choi ◽  
Eunjoo Kim ◽  
Seong-Kyoon Choi ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Mice ◽  
Transplantation Outcomes

scholarly journals Comparison of the Effects of Liraglutide on Islet Graft Survival Between Local and Systemic Delivery

2020 ◽  
Vol 29 ◽  
pp. 096368972097124
Author(s):  
Song Mi Lee ◽  
Donghee Kim ◽  
Kyung Min Kwak ◽  
Phyu Phyu Khin ◽  
Oh Kyung Lim ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Mouse ◽  
Diabetic Mice ◽  
Systemic Delivery ◽  
Systemic Injection ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Promising Treatment ◽  
Glucagon Like Peptide 1 ◽  
Islet Survival

Islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus. Liraglutide, a glucagon-like peptide-1 (GLP-1) receptor agonist, protects beta cells after islet transplantation by improving glycemic control through several mechanisms. In this study, we compared the effects of local pretreatment and systemic treatment with liraglutide on islet transplantation in a diabetic mouse model. Streptozotocin (STZ)-induced diabetic C57BL/6 mice were transplanted with syngeneic islets under the kidney capsule. Isolated islets were either locally treated with liraglutide before transplantation or mice were treated systemically by intraperitoneal injection after islet transplantation. Local pretreatment of islets with liraglutide was more effective in increasing body weight, decreasing hemoglobin A1c levels, and lowering blood glucose levels in STZ-diabetic mice transplanted with islets. Local pretreatment was also more effective in increasing insulin secretion and islet survival in STZ-diabetic mice. Histological analysis of the transplantation site revealed fewer apoptotic cells following local pretreatment compared with systemic injection of liraglutide. These findings indicate that liraglutide administered once locally before transplantation might have superior effects on islet preservation than systemic administration.

Immunosuppressive PLGA TGF-ß1 Microparticles Induce Antigen-Specific T-Cell Tolerance for Enhancing Islet Transplantation Outcomes

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 31-OR
Author(s):  
YING LI ◽  
ANTHONY FREI ◽  
STEVEN D. BARASH ◽  
CHERIE L. STABLER
Keyword(s):  
T Cell ◽  
Islet Transplantation ◽  
T Cell Tolerance ◽  
Cell Tolerance ◽  
Transplantation Outcomes

2290-PUB: Donor HLA-DR4 Positivity and Islet Transplantation Outcomes

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2290-PUB
Author(s):  
CARMEN CHAVEZ ◽  
VIRGINIA FUENMAYOR ◽  
DAVID BAIDAL ◽  
ANA M. ALVAREZ ◽  
NATHALIA PADILLA ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Transplantation Outcomes

scholarly journals Extra-Hepatic Islet Transplantation

2018 ◽  
Vol 27 (8) ◽  
pp. 1289-1293 ◽  
Author(s):  
Anaïs Schaschkow ◽  
Séverine Sigrist ◽  
Carole Mura ◽  
Caroline Dissaux ◽  
Karim Bouzakri ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Cell Therapy ◽  
Islet Transplantation ◽  
Graft Function ◽  
Surgical Techniques ◽  
Diabetic Rats ◽  
C Peptide ◽  
And Function ◽  
Islet Survival

Following the tremendous development of hydrogels for cell therapy, there is now a growing need for surgical techniques to validate in vivo scaffold benefits for islet transplantation. Therefore, we propose a newly designed surgical procedure involving the injection of hydrogel-embedded pancreatic islets in the omentum, which is considered a favorable environment for cell survival and function. Our technique, called h-Omental Matrix Islet filliNG (hOMING) was designed to test the benefits of hydrogel on islet survival and function in vivo. Islets were implanted in the omentum of diabetic rats using the hOMING technique and alginate as an islet carrier. Blood glucose and C-peptide levels were recorded to assess graft function. After 2 months, grafts were explanted and studied using insulin and vessel staining. All rats that underwent hOMING exhibited graft function characterized by a glycemia decrease and a C-peptidemia increase ( P < 0.001 compared with preoperative levels). Furthermore, hOMING appeared to preserve islet morphology and insulin content and allowed the proper revascularization of grafted islets. The results suggest that hOMING is a viable and promising approach to test in vivo the benefits of hydrogel administration for islet transplantation into the omental tissue.

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