scholarly journals The Spatio-Temporal Control of Zygotic Genome Activation

2018 ◽  
Author(s):  
George Gentsch ◽  
Nick D. L. Owens ◽  
James C. Smith
Keyword(s):  
Temporal Control ◽  
Zygotic Genome Activation ◽  
Spatio Temporal ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals The Spatio-Temporal Control of Zygotic Genome Activation

2018 ◽  
Author(s):  
George E. Gentsch ◽  
Nick D. L. Owens ◽  
James C. Smith
Keyword(s):  
Cell Cycle ◽  
Rna Polymerase Ii ◽  
Temporal Dynamics ◽  
Bmp Signaling ◽  
Cycle Duration ◽  
Zygotic Genome Activation ◽  
Significant Events ◽  
Spatio Temporal ◽  
Genome Activation ◽  
Zygotic Genome

SUMMARYOne of the earliest and most significant events in embryonic development is zygotic genome activation (ZGA). In several species, bulk transcription begins at the mid-blastula transition (MBT) when, after a certain number of cleavages, the embryo attains a particular nuclear-to-cytoplasmic (N/C) ratio, maternal repressors become sufficiently diluted, and the cell cycle slows down. Here we resolve the frog ZGA in time and space by profiling RNA polymerase II (RNAPII) engagement and its transcriptional readout. We detect a gradual increase in both the quantity and the length of RNAPII elongation before the MBT, revealing that >1,000 zygotic genes disregard the N/C timer for their activation, and that the sizes of newly transcribed genes are not necessarily constrained by cell cycle duration. We also find that Wnt, Nodal and BMP signaling together generate most of the spatio-temporal dynamics of regional ZGA, directing the formation of orthogonal body axes and proportionate germ layers.

CLAMP regulates Zygotic Genome Activation in Drosophila embryos

Genetics ◽  
2021 ◽  
Author(s):  
Megan M Colonnetta ◽  
Juan E Abrahante ◽  
Paul Schedl ◽  
Daryl M Gohl ◽  
Girish Deshpande
Keyword(s):  
Temporal Dynamics ◽  
Embryonic Patterning ◽  
Zygotic Genome Activation ◽  
Promoter Sequences ◽  
Genome Wide ◽  
Zygotic Transcription ◽  
Pioneer Factor ◽  
Genome Activation ◽  
Drosophila Embryos ◽  
Zygotic Genome

Abstract Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here we have explored novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands of sites genome-wide throughout early embryogenesis. Interestingly, CLAMP relocates to target promoter sequences across the genome when ZGA is initiated. Although there is a considerable overlap between CLAMP and Zelda binding sites, the proteins display distinct temporal dynamics. To assess whether CLAMP occupancy affects gene expression, we analyzed transcriptomes of embryos zygotically compromised for either clamp or zelda and found that transcript levels of many zygotically-activated genes are similarly affected. Importantly, compromising either clamp or zelda disrupted the expression of critical segmentation and sex determination genes bound by CLAMP (and Zelda). Furthermore, clamp knockdown embryos recapitulate other phenotypes observed in Zelda-depleted embryos, including nuclear division defects, centrosome aberrations, and a disorganized actomyosin network. Based on these data, we propose that CLAMP acts in concert with Zelda to regulate early zygotic transcription.

scholarly journals Zygotic Genome Activation in Vertebrates

2017 ◽  
Vol 42 (4) ◽  
pp. 316-332 ◽  
Author(s):  
David Jukam ◽  
S. Ali M. Shariati ◽  
Jan M. Skotheim
Keyword(s):  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

MouseXist expression begins at zygotic genome activation and is timed by a zygotic clock

2001 ◽  
Vol 61 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Maurizio Zuccotti ◽  
Michele Boiani ◽  
Ruben Ponce ◽  
Stefano Guizzardi ◽  
Renato Scandroglio ◽  
...  
Keyword(s):  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

Cell Reports ◽  
2019 ◽  
Vol 27 (10) ◽  
pp. 2962-2977.e5 ◽  
Author(s):  
Kitt D. Paraiso ◽  
Ira L. Blitz ◽  
Masani Coley ◽  
Jessica Cheung ◽  
Norihiro Sudou ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome
Sign in / Sign up

Export Citation Format

Share Document