scholarly journals Transient Intracellular Acidification Regulates the Core Transcriptional Heat Shock Response

2018 ◽  
Author(s):  
Catherine G. Triandafillou ◽  
Christopher D. Katanski ◽  
Aaron R. Dinner ◽  
D. Allan Drummond
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
Intracellular Acidification ◽  
The Core ◽  
Shock Response

scholarly journals Transient intracellular acidification regulates the core transcriptional heat shock response

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Catherine G Triandafillou ◽  
Christopher D Katanski ◽  
Aaron R Dinner ◽  
D Allan Drummond
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Heat Shock Factor 1 ◽  
Transcriptional Program ◽  
Intracellular Acidification ◽  
The Core ◽  
Cell Cycle Reentry ◽  
Shock Response ◽  
Cytosolic Acidification

Heat shock induces a conserved transcriptional program regulated by heat shock factor 1 (Hsf1) in eukaryotic cells. Activation of this heat shock response is triggered by heat-induced misfolding of newly synthesized polypeptides, and so has been thought to depend on ongoing protein synthesis. Here, using the budding yeast Saccharomyces cerevisiae, we report the discovery that Hsf1 can be robustly activated when protein synthesis is inhibited, so long as cells undergo cytosolic acidification. Heat shock has long been known to cause transient intracellular acidification which, for reasons which have remained unclear, is associated with increased stress resistance in eukaryotes. We demonstrate that acidification is required for heat shock response induction in translationally inhibited cells, and specifically affects Hsf1 activation. Physiological heat-triggered acidification also increases population fitness and promotes cell cycle reentry following heat shock. Our results uncover a previously unknown adaptive dimension of the well-studied eukaryotic heat shock response.

scholarly journals Transient intracellular acidification regulates the core transcriptional heat shock response

2018 ◽  
Author(s):  
Catherine G. Triandafillou ◽  
Christopher D. Katanski ◽  
Aaron R. Dinner ◽  
D. Allan Drummond
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Heat Shock Factor 1 ◽  
Transcriptional Program ◽  
Intracellular Acidification ◽  
The Core ◽  
Cell Cycle Reentry ◽  
Shock Response ◽  
Cytosolic Acidification

AbstractHeat shock induces a conserved transcriptional program regulated by heat shock factor 1 (Hsf1) in eukaryotic cells. Activation of this heat-shock response is triggered by heat-induced misfolding of newly synthesized polypeptides, and so has been thought to depend on ongoing protein synthesis. Here, using the the budding yeastSaccharomyces cerevisiae, we report the discovery that Hsf1 can be robustly activated when protein synthesis is inhibited, so long as cells undergo cytosolic acidification. Heat shock has long been known to cause transient intracellular acidification which, for reasons which have remained unclear, is associated with increased stress resistance in eukaryotes. We demonstrate that acidification is required for heat shock response induction in translationally inhibited cells, and specifically affects Hsf1 activation. Physiological heat-triggered acidification also increases population fitness and promotes cell cycle reentry following heat shock. Our results uncover a previously unknown adaptive dimension of the well-studied eukaryotic heat shock response.

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