Starvation Elicits PspA-Dependent Phenotypic Antibiotic Tolerance in Escherichia Coli

2018 ◽  
Author(s):  
Miaomiao Wang ◽  
Edward Wai Chi Chan ◽  
Marcus Ho-yin Wong ◽  
Sheng Chen
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Tolerance

scholarly journals Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance

2010 ◽  
Vol 10 (1) ◽  
pp. 185 ◽  
Author(s):  
Trevor R Zuroff ◽  
Hans Bernstein ◽  
Jenna Lloyd-Randolfi ◽  
Lourdes Jimenez-Taracido ◽  
Philip S Stewart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Robustness Analysis ◽  
Aminoglycoside Antibiotic ◽  
Antibiotic Tolerance ◽  
Beta Lactam

scholarly journals Role of Global Regulators and Nucleotide Metabolism in Antibiotic Tolerance in Escherichia coli

2008 ◽  
Vol 52 (8) ◽  
pp. 2718-2726 ◽  
Author(s):  
Sonja Hansen ◽  
Kim Lewis ◽  
Marin Vulić
Keyword(s):  
Escherichia Coli ◽  
Cell Formation ◽  
Nucleotide Metabolism ◽  
Flavin Mononucleotide ◽  
Formation Mechanisms ◽  
Antibiotic Tolerance ◽  
Persister Cells ◽  
Global Regulators ◽  
Persister Cell

ABSTRACT Bacterial populations produce a small number of persister cells that exhibit multidrug tolerance. Persister cells are largely responsible for the antibiotic recalcitrance of biofilm infections. The mechanism of persister cell formation largely remains unknown due to the challenges in identifying persister genes. We screened an ordered comprehensive library of 3,985 Escherichia coli knockout strains to identify mutants with altered antibiotic tolerance. Stationary-state cultures in 96-well plates were exposed to ofloxacin at a concentration which allows only tolerant persister cells to survive. The persister cell level of each culture was determined. A total of 150 mutants with decreased persistence were identified in the initial screen, and subsequent validation confirmed that neither the growth rate nor the ofloxacin MIC was affected for 10 of them. The genes affected in these strains were dnaJ and dnaK (chaperones), apaH (diadenosine tetraphosphatase), surA (peptidyl-prolyl cis-trans isomerase), fis and hns (global regulators), hnr (response regulator of RpoS), dksA (transcriptional regulator of rRNA transcription), ygfA (5-formyl-tetrahydrofolate cyclo-ligase), and yigB (flavin mononucleotide [FMN] phosphatase). The prominent presence of global regulators among these strains pointed to the likely redundancy of persister cell formation mechanisms: the elimination of a regulator controlling several redundant persister genes would be expected to produce a phenotype. This observation is consistent with previous findings for a possible role of redundant genes such as toxin/antitoxin modules in persister cell formation. ygfA and yigB were of special interest. The mammalian homolog of YgfA (methenyltetrahydrofolate synthetase) catalyzes the conversion of 5-formyl-tetrahydrofolate (THF) into the rapidly degraded 5,10-methenyl-THF, depleting the folate pool. The YigB protein is a phosphatase of FMN which would deplete the pool of this cofactor. Stochastic overexpression of these genes could lead to dormancy and, hence, tolerance by depleting the folate and FMN pools, respectively. Consistent with this scenario, the overexpression of both genes produced increased tolerance to ofloxacin.

scholarly journals Salmonella typhimurium intercepts Escherichia coli signaling to enhance antibiotic tolerance

2013 ◽  
Vol 110 (35) ◽  
pp. 14420-14425 ◽  
Author(s):  
N. M. Vega ◽  
K. R. Allison ◽  
A. N. Samuels ◽  
M. S. Klempner ◽  
J. J. Collins
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Antibiotic Tolerance

scholarly journals Aminoglycosides induce a bacterial senescent state that increases antibiotic tolerance in treatment-naïve cells

2021 ◽  
Author(s):  
Christian T. Meyer ◽  
Giancarlo N. Bruni ◽  
Ben Dodd ◽  
Joel M. Kralj
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Bacterial Species ◽  
Genetic Resistance ◽  
Membrane Integrity ◽  
Antibiotic Tolerance ◽  
Paracrine Signaling ◽  
E Coli ◽  
Evolutionary Innovation ◽  
Treatment Naïve

Bacterial evolution of antibiotic resistance is facilitated by non-genetic resistance that increases drug tolerance, buying time for evolutionary innovation. Escherichia coli treated with aminoglycosides permanently lose the ability to divide within four hours, yet we discovered a majority of cells maintain membrane integrity and metabolic activity greater than two days post treatment — a bacterial senescent-like state. These cells, which we term zombies, exhibit dynamic gene expression and metabolomic profiles, even after irreversible exit from the cell cycle. Our data reveal zombies upregulate the phage shock protein pathway to maintain membrane integrity. Remarkably, though unable to form new colonies, zombies increase the antibiotic tolerance of treatment-naïve cells, implying chemical communication. Chemical supplementation and genetic knockouts show that zombies communicate with treatment-naïve cells by secreting indole. In summary, our study revealed a bacterial senescent-like state, induced by aminoglycosides, that decreases the antibiotic susceptibility of multiple bacterial species. Thus, E. coli zombies utilize paracrine signaling to promote non-genetic antibiotic tolerance.

scholarly journals PasT of Escherichia coli sustains antibiotic tolerance and aerobic respiration as a bacterial homolog of mitochondrial Coq10

2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Cinzia Fino ◽  
Martin Vestergaard ◽  
Hanne Ingmer ◽  
Fabien Pierrel ◽  
Kenn Gerdes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aerobic Respiration ◽  
Antibiotic Tolerance ◽  
Bacterial Homolog

The Two-Component System ZraPSR Is a Novel ESR that Contributes to Intrinsic Antibiotic Tolerance in Escherichia coli

2018 ◽  
Vol 430 (24) ◽  
pp. 4971-4985 ◽  
Author(s):  
Kévin Rome ◽  
Céline Borde ◽  
Raleb Taher ◽  
Julien Cayron ◽  
Christian Lesterlin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Tolerance ◽  
Two Component System ◽  
Component System ◽  
Two Component

scholarly journals Joint Tolerance to β-Lactam and Fluoroquinolone Antibiotics in Escherichia coli Results from Overexpression of hipA

1998 ◽  
Vol 42 (12) ◽  
pp. 3282-3284 ◽  
Author(s):  
Timothy J. Falla ◽  
Ian Chopra
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Cell Division ◽  
Chromosome Replication ◽  
Antibiotic Tolerance ◽  
Fluoroquinolone Antibiotics

ABSTRACT The basis of joint tolerance to β-lactam and fluoroquinolone antibiotics in Escherichia coli mediated byhipA was examined. An antibiotic tolerance phenotype was produced by overexpression of hipA under conditions that did not affect the growth rate of the organism. OverexpressinghipA probably decreases the period in which bacteria are susceptible to the antibiotics by temporarily affecting some aspect of chromosome replication or cell division.

Sign in / Sign up

Export Citation Format

Share Document