Drastic Short-Term Changes in the Isotopic Composition of Soil Nitrate in Forest Soil Samples

2008 ◽  
Vol 72 (6) ◽  
pp. 1645 ◽  
Author(s):  
Heidi C. Hales ◽  
Donald S. Ross
Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 569
Author(s):  
Chakriya Sansupa ◽  
Sara Fareed Mohamed Wahdan ◽  
Terd Disayathanoowat ◽  
Witoon Purahong

This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.


2014 ◽  
Vol 102 (7) ◽  
Author(s):  
Merja Lusa ◽  
Janne Lempinen ◽  
Hanna Ahola ◽  
Mervi Söderlund ◽  
Anne-Maj Lahdenperä ◽  
...  

AbstractSoil samples from three forest soil pits were examined down to a depth of approximately three metres using 1 M ammonium acetate extraction and microwave-assisted extraction with concentrated nitric acid (HNO


PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210623 ◽  
Author(s):  
Bettina Tonn ◽  
Ina Porath ◽  
Fernando A. Lattanzi ◽  
Johannes Isselstein

Soil Science ◽  
2019 ◽  
Vol 184 (2) ◽  
pp. 35-42 ◽  
Author(s):  
Federica Borgogni ◽  
Anna Lavecchia ◽  
Giovanni Mastrolonardo ◽  
Giacomo Certini ◽  
Maria Teresa Ceccherini ◽  
...  

1996 ◽  
Vol 181 (2) ◽  
pp. 147-159 ◽  
Author(s):  
J. Arunachalam ◽  
H. Emons ◽  
B. Krasnodebska ◽  
C. Mohl

FLORESTA ◽  
2014 ◽  
Vol 44 (4) ◽  
pp. 735 ◽  
Author(s):  
Ivanildo Amorim de Oliveira ◽  
José Marques Júnior ◽  
Milton César Costa Campos ◽  
Renato Eleotério de Aquino ◽  
Diego Silva Siqueira ◽  
...  

AbstractConsidering the lack of information about spatial behavior of the soil attributes in areas of archaeological black earth and native forest, the objective of this study was to evaluate the spatial variability of chemical attributes and determine the sampling density in soil with archaeological black earth and native forest in the region of Manicoré, AM. The study was conducted in a rural property located in the community of Santo Antônio do Matupi, at the margins of BR 230, Trans-amazon highway, in the region of Manicoré, AM. In these areas were established grids of 70 m x 70 m, with regular spacing of 10 x 10 m, totaling 64 points, then soil samples were collected at a depth of 0.0-0.20 m and 0 , 40 - 0,60 m. Chemical attributes were determined (pH, OM, P, K, Ca, Mg, SB, CTC, V% and H + Al). Data were analyzed using descriptive statistical techniques and geostatistics. Sampling density was determined basing on CV and on the range of the semivariograms. It was verified that the studied attributes showed spatial variability and the area of archaeological black earth presented greater spatial variability than the native forest. Its greater sampling density was determined basing on the range of the adjusted semivariograms.Keywords: Indian black earth; attributes of soil; geostatistics. 


2012 ◽  
Vol 77 (6) ◽  
pp. 833-843 ◽  
Author(s):  
Ivica Djalovic ◽  
Djordje Jockovic ◽  
Goran Dugalic ◽  
Goran Bekavac ◽  
Bozana Purar ◽  
...  

Soil acidity and aluminum toxicity are considered most damaging soil conditions affecting the growth of most crops. This paper reviews the results of tests of pH, exchangeable acidity and mobile aluminum (Al) concentration in profiles of pseudogley soils from Cacak-Kraljevo basin. For that purpose, 102 soil pits were dug in 2009 in several sites around Cacak- Kraljevo basin. The tests encompassed 54 field, 28 meadow, and 20 forest soil samples. Samples of soil in the disturbed state were taken from the Ah and Eg horizons (102 samples), from the B1tg horizon in 39 field, 24 meadow and 15 forest pits (a total of 78 samples) and from the B2tg horizon in 14 field, 11 meadow, and 4 forest pits (a total of 29 samples). Mean pH values (1M KCl) of the tested soil profiles were 4.28, 3.90 and 3.80 for the Ah, Eg and B1tg horizons, respectively. Soil pH of forest samples was lower than those in meadow and arable land samples (mean values of 4.06, 3.97 and 3.85 for arable land, meadow and forest samples, respectively). Soil acidification was especially intensive in deep horizons, as 27% (Ah), 77% (Eg) and 87% (B1tg) soil samples had the pH value below 4.0. Mean values of total exchangeable acidity (TEA) were 1.55, 2.33 and 3.40 meq 100 g-1 for the Ah, Eg and B1tg horizons, respectively. The TEA values in forest soils were considerably higher (3.39 meq 100 g-1) than those in arable soils and meadow soils (1.96 and 1.93, respectively). Mean mobile Al contents of tested soil samples were 11.02, 19.58 and 28.33 mg Al 100 g-1 for the Ah, Eg and B1tg horizons, respectively. According to the pH and TEA values, mobile Al was considerably higher in the forest soils (the mean value of 26.08 mg Al 100 g-1) than in the arable soils and meadow soils (the mean values of 16.85 and 16.00 mg Al 100 g-1, respectively). The Eg and B1tg horizons of the forest soil had especially high mobile Al contents (the mean values of 28.50 and 32.95 mg Al 100 g-1, respectively). High levels of mobile Al were especially frequent in the forest soils, with 35% (Ah), 85.0% (Eg) and 93.3% (B1tg) of the tested samples ranging above 10 mg Al 100 g-1.


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