Marker‐Assisted Selection for the Broad‐Spectrum Potato Late Blight Resistance Conferred by Gene RB Derived from a Wild Potato Species

Crop Science ◽  
2006 ◽  
Vol 46 (2) ◽  
pp. 589-594 ◽  
Author(s):  
Lara M. Colton ◽  
Horia I. Groza ◽  
Susan M. Wielgus ◽  
Jiming Jiang
Keyword(s):  
Late Blight ◽  
Broad Spectrum ◽  
Marker Assisted Selection ◽  
Late Blight Resistance ◽  
Potato Late Blight ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species ◽  
Selection For

scholarly journals Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species Solanum microdontum and Solanum pampasense

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 732
Author(s):  
Fergus Meade ◽  
Ronald Hutten ◽  
Silke Wagener ◽  
Vanessa Prigge ◽  
Emmet Dalton ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Genes ◽  
Late Blight Resistance ◽  
Gene Clusters ◽  
Genetic Maps ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species ◽  
Solanum Microdontum

Wild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the resistance can be identified and tagged using molecular markers. In this study, F1 populations were created from crosses between blight susceptible parents and lines exhibiting strong partial resistance to late blight derived from the South American wild species Solanum microdontum and Solanum pampasense. Both populations exhibited continuous variation for resistance to late blight over multiple field-testing seasons. High density genetic maps were created using single nucleotide polymorphism (SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were consistently expressed over multiple years in both populations. In the population created with the S. microdontum source, QTLs for resistance consistently expressed over three years and explaining a large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016 only. In the population created with the S. pampasense source, QTLs for resistance were found in over two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic models following the effect of the presence or absence of SNPs associated with consistently effective loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress and pyramid these loci have potential in resistance breeding strategies.

Late blight resistance status in wild potato species against Indian population of Phytophthora infestans

2018 ◽  
Vol 75 (1) ◽  
pp. 99 ◽  
Author(s):  
Vinay Bhardwaj ◽  
Dalamu ◽  
A.K. Srivastava ◽  
Sanjeev Sharma ◽  
Vinod Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Indian Population ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species

Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana)

2005 ◽  
Vol 111 (6) ◽  
pp. 1201-1214 ◽  
Author(s):  
F.G. Villamon ◽  
D.M. Spooner ◽  
M. Orrillo ◽  
E. Mihovilovich ◽  
W. Pérez ◽  
...  
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species

scholarly journals LATE BLIGHT RESISTANCE OF WILD POTATO SPECIES UNDER FIELD CONDITIONS IN THE NORTHWEST OF RUSSIA

2020 ◽  
Vol 180 (4) ◽  
pp. 159-169 ◽  
Author(s):  
N. M. Zoteyeva
Keyword(s):  
Late Blight ◽  
Natural Infection ◽  
Late Blight Resistance ◽  
Field Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species ◽  
Wide Range ◽  
Foliar Resistance ◽  
High Level

Background. Despite the great efforts made by breeders, late blight remains a paramount cause of significant potato harvest losses. Introgression of various resistance genes from wild Solanum L. species is the main method to increase the resistance in potato cultivars. Field resistance is considered to be more durable than those induced by the action of single R genes. To this end, resistance sources should be selected from а wide range of species under severe natural infection.Material and methods. As the material for evaluation, 1141 accessions of 99 wild potato species belonging to 15 taxonomic series according to the system of J. Hawkes were used. Each accession was assessed for 3–5 years. A 1–9 point scale was employed to score the damage of plants every week starting from the first symptoms of the disease, where 9 meant the absence of any symptoms, and 1 the entirely damaged plant. The plants scoring 6 to 9 points were considered resistant.Results and conclusions. As a result of the long-term field observations, wild potato species, represented in the current evaluation by numerous accessions, were characterized for foliar resistance to late blight; individual introductions resistant to late blight were also identified. Some of those studied in the 1980s showed high resistance in the end of the 1990s through the 2000s. The highest percentage of resistant accessions/species was identified among the species with areas of distribution in Mexico. A group of Central American species and large part of species with areas of distribution in South America expressed high level of interspecific polymorphism in foliar resistance to late blight.

Transcriptome analysis of scions grafted to potato rootstock for improving late blight resistance

2020 ◽  
Author(s):  
Yuexin Li ◽  
Degang Zhao
Keyword(s):  
Disease Resistance ◽  
Late Blight ◽  
Protein Kinases ◽  
Molecular Mechanisms ◽  
Late Blight Resistance ◽  
Potato Late Blight ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Blight Resistance ◽  
Expression Levels

Abstract Background: Late blight seriously threatens potato cultivation worldwide. The severe and widespread damage caused by the fungal pathogen can lead to drastic decreases in potato yield. Although grafting technology has been widely used to improve crop resistance, the effects of grafting on potato late blight resistance as well as the associated molecular mechanisms remain unclear. Therefore, we performed RNA transcriptome sequencing analysis and the late blight resistance testing of the scion when the potato late blight-resistant variety Qingshu 9 and the susceptible variety Favorita were used as the rootstock and scion, respectively, and vice versa. The objective of this study was to evaluate the influence of the rootstock on scion disease resistance and to clarify the related molecular mechanisms.Results: A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the expression levels of genes related to plant–pathogen interactions, plant mitogen-activated protein kinase (MAPK) signaling pathways, and plant hormone signal transduction pathways were significantly up-regulated in the scion when Qingshu 9 was used as the rootstock. These genes included late blight response genes encoding calcium-dependent protein kinases (CDPKs), chitin elicitor receptor kinases (CERKs), LRR receptor serine/threonine protein kinases (LRR-LRKs), NPR family proteins in the salicylic acid synthesis pathway, and MAPKs. When Favorita was used as the rootstock, the expression levels of the late blight response genes were not up-regulated in the Qingshu 9 scion, but the expression levels of the genes related to proline metabolism, fatty acid chain elongation, and diterpenoid biosynthesis pathways were down-regulated. Resistance results showed that self-grafting of the susceptible variety and grafting with the resistant variety as the rootstock increased the resistance of the susceptible scion to late blight. However, the resistance was stronger after grafting with the resistant variety as the rootstock. Using the susceptible variety as the rootstock decreased the late blight resistance of the resistant scion.Conclusions: Our results showed that changes to the expression of disease resistance genes in the scion after grafting are associated with late blight resistance. The results provide the basis for exploring the molecular mechanism underlying the effects of rootstocks on scion disease resistance.

Allele Mining in Solanum Germplasm: Cloning and Characterization of RB-Homologous Gene Fragments from Late Blight Resistant Wild Potato Species

2015 ◽  
Vol 33 (5) ◽  
pp. 1584-1598 ◽  
Author(s):  
Jagesh Kumar Tiwari ◽  
Sapna Devi ◽  
Sanjeev Sharma ◽  
Poonam Chandel ◽  
Shashi Rawat ◽  
...  
Keyword(s):  
Late Blight ◽  
Homologous Gene ◽  
Wild Potato ◽  
Allele Mining ◽  
Wild Potato Species ◽  
Potato Species

scholarly journals Competition between Phytophthora infestans Effectors Leads to Increased Aggressiveness on Plants Containing Broad-Spectrum Late Blight Resistance

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10536 ◽  
Author(s):  
Dennis A. Halterman ◽  
Yu Chen ◽  
Jiraphan Sopee ◽  
Julio Berduo-Sandoval ◽  
Amilcar Sánchez-Pérez
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Broad Spectrum ◽  
Late Blight Resistance ◽  
Blight Resistance

scholarly journals Performance of Transgenic Potato Containing the Late Blight Resistance Gene RB

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Dennis A. Halterman ◽  
Lara Colton Kramer ◽  
Susan Wielgus ◽  
Jiming Jiang
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Transgenic Potato ◽  
Field Trials ◽  
Blight Resistance ◽  
Solanum Bulbocastanum ◽  
Wild Potato Species ◽  
Foliar Resistance ◽  
In The Wild

Late blight of potato, caused by Phytophthora infestans, is one of the most devastating diseases of potato. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum through map-based cloning. The full-length gene coding sequence, including the open reading frame and promoter, has been integrated into cultivated potato (S. tuberosum) using Agrobacterium-mediated transformation. RB-containing transgenic plants were challenged with P. infestans under optimal late blight conditions in greenhouse experiments. All transgenic lines containing RB exhibited strong foliar resistance. Field-grown transgenic tubers also were tested for resistance to P. infestans. In contrast to the foliar resistance phenotype, RB-containing tubers did not exhibit increased resistance. Two years of field trials were used to ascertain whether the presence of RB had any effect on tuber yield. We were unable to detect any significant effect on tuber size or yield after addition of the resistance gene to several S. tuberosum cultivars.

scholarly journals The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato

2005 ◽  
Vol 44 (2) ◽  
pp. 208-222 ◽  
Author(s):  
Edwin A.G. Vossen ◽  
Jack Gros ◽  
Anne Sikkema ◽  
Marielle Muskens ◽  
Doret Wouters ◽  
...  
Keyword(s):  
Late Blight ◽  
Broad Spectrum ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Solanum Bulbocastanum
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