Sensitivity Analysis of Computer-Based Diameter Measurement from Digital Images

Crop Science ◽  
2003 ◽  
Vol 43 (2) ◽  
pp. 583 ◽  
Author(s):  
Richard W. Zobel
Keyword(s):  
Sensitivity Analysis ◽  
Digital Images ◽  
Diameter Measurement ◽  
Computer Based

Hematopoietic Colony Forming Unit: Development of a High-Throughput CFU Assay Strategy by the Use of High-Resolution Digital Images Stored in a Laboratory Information System.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 2306-2306 ◽  
Author(s):  
Maria S Albano ◽  
William Rothman ◽  
Chiseko Watanabe ◽  
Anna Gora ◽  
Andromachi Scaradavou ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Digital Imaging ◽  
Digital Images ◽  
Imaging System ◽  
Information Management System ◽  
Traditional Classification ◽  
New Strategy ◽  
One Step ◽  
Computer Based

Abstract Background: Umbilical Cord Blood (CB) is an increasingly accepted graft source for patients lacking related donors. Graft characteristics currently used as determinants of quality and engraftment potential of CB units include the enumeration of Total Nucleated Cell (TNC), CD34+ and Colony Forming Units (CFU). Among those, the 14 day-CFU assay is the only one that determines the functional state as well as the number of hematopoietic progenitor cells. Thus, CFU in pre and post cryopreservation/thawing specimens are strong independent predictors of CB graft engraftment. Traditionally, evaluation of CFU growth is performed by light microscopy (traditional classification), is time consuming, subjective and difficult to standardize. Aims: A) To evaluate whether the incorporation of high-resolution digital imaging and colony staining could make reading the traditional CFU assay objective and readily standardizable. B) To validate the new strategy vs traditional classification. C) To develop a computer based laboratory information management system (LIMS) to support high-throughput CFU assay. Methods and Results: After 14 days of CB culture (CFU assay-Stem Cell Technologies), an image of the 35 mm culture dishes was captured using a high-resolution photographic camera based digital imaging system, which achieves a resolution of 7.6 μM per pixel and thus, allows a clear view of all colonies in the dish with their barcoded IDs. A short one-step staining with MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide) allows an even better definition of CFU-GM/E; CFU-GM and CFU-E by bestowing a specific color on each type (dark purple, purple and red respectively) against a uniformly clear background. A good correlation was observed after comparison of the new strategy against traditional enumeration (R2 linear= 0.95; n= 122 culture dishes evaluated). Low variation was observed after 151 cultures were independently classified and enumerated by three different operators (CV%= 8.9; range 1–27%). Sample plating introduced variation of the CFU assay, in an experiment where nine CB samples were evaluated by multiple plating (Intra-assay CV%= 21.9 %; range 3.4–34.5% and Inter-assay CV%= 23.3%; range 12.6–35%). A computer based laboratory information (LIMS) was developed to store all culture dishes, linked by unique barcoded ID labels to a specific CB unit, and including CB image, incubator location, plating and counting dates, as well as detailed colony enumeration. This system has been used for 5 months in our laboratory and more than 3,000 CB units have been tested in duplicate (average: 30 CB/day). Images of an average of 60 culture dishes and MTT staining can be performed in less than two hours. The specific coloration of CFU colonies allows faster classification and enumeration and thus, permits a more precise analysis of CFU colonies and its relation with CD34+ cell content and post-transplant engraftment. Summary: With this new strategy, CFU can be objectively visualized, differentiated and counted; the digital images can be stored for future review and refined classification. The described system provides computerized information on optical assay parameters and has become an invaluable tool supporting high-throughput implementation. Thus, the combination of high resolution imaging, one-step staining and the traditional CFU assay overcome most technical challenges of the conventional method, supporting standardization and yielding high reproducibility to the assay which can be easily implemented in CB banks where large numbers of samples need to be tested daily.

scholarly journals The Effectiveness of Saireito, a Traditional Japanese Herbal Medicine, in Reducing Postoperative Edema after Acquired Ptosis Surgery: A Prospective Controlled Trial

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Naoki Morimoto ◽  
Natsuko Kakudo ◽  
Toshihito Mitsui ◽  
Atsuyuki Kuro ◽  
Yuko Ueda ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Digital Images ◽  
Controlled Trial ◽  
Controlled Study ◽  
Control Group ◽  
Common Complication ◽  
Visual Analog Scales ◽  
Japanese Herbal Medicine ◽  
Computer Based ◽  
Ptosis Surgery

Background. Persistent edema is a common complication after the surgical treatment of blepharoptosis; however, no objective methods have been established for evaluating or treating this condition. We focused on the Japanese herbal medicine, Saireito, and evaluated its efficacy in reducing postoperative edema. Methods. This was a prospective, nonrandomized, and controlled study. We evaluated the incidence of postoperative edema in a Control group using a subjective patient-assessed visual analog scales (VASs) to assess swelling, pain, itching, and local warmth and an objective surgeon-assessed VAS to evaluate swelling. Swelling was also assessed by an objective computer-based analysis of digital images. These methods were used to evaluate the effects of Saireito (8.1 g/day) for 8 weeks. Results. A total of 49 patients and 80 eyelids were enrolled. Twenty-nine patients and 48 eyelids were assigned to the Control group, and 20 patients and 32 eyelids were assigned to the Saireito group. Our analysis of the Control group indicated that postoperative edema persisted for up to 8 weeks. On the other hand, the postoperative edema in the Saireito group was mostly eliminated at 8 weeks. The computer-based analysis of digital images showed that the edema tended to be reduced in the Saireito group compared with the Control group. Conclusion. Saireito might be effective for reducing postoperative edema after blepharoptosis surgery and almost completely eliminated it at 8 weeks after surgery. This study was a preliminary and nonrandomized study; therefore, the randomized and placebo-controlled study will be needed in the next step.

scholarly journals Acetate Templating on Digital Images Is More Accurate Than Computer-based Templating for Total Hip Arthroplasty

2015 ◽  
Vol 473 (12) ◽  
pp. 3752-3759 ◽  
Author(s):  
Robert Petretta ◽  
Jason Strelzow ◽  
Nicholas E. Ohly ◽  
Peter Misur ◽  
Bassam A. Masri
Keyword(s):  
Total Hip Arthroplasty ◽  
Hip Arthroplasty ◽  
Digital Images ◽  
Total Hip ◽  
Computer Based

Development of a Computer-Based Burn Diagram from Digital Images

2002 ◽  
Vol 23 ◽  
pp. S88 ◽  
Author(s):  
A. P. Sanford ◽  
S. Sanford ◽  
D. N. Herndon
Keyword(s):  
Digital Images ◽  
Computer Based

scholarly journals Sensitivity analysis for correlation-based registration of digital images

1998 ◽  
Vol 27 (9-11) ◽  
pp. 243-255 ◽  
Author(s):  
G. Waldman ◽  
J.R. Wootton ◽  
A. García-Ortiz
Keyword(s):  
Sensitivity Analysis ◽  
Digital Images

scholarly journals Computer art in Italia negli anni Ottanta Tecnologia, matematica e immaginario scientifico

Venezia Arti ◽  
2020 ◽  
Author(s):  
Paola Lagonigro
Keyword(s):  
Digital Images ◽  
Scientific Evidence ◽  
Scientific Research ◽  
Personal Computers ◽  
Computer Art ◽  
Scientific Culture ◽  
Art Scene ◽  
Mathematical Formulas ◽  
New Type ◽  
Computer Based

In the 1980s, the spread of personal computers gave impetus to the birth of a varied computer-based art scene. While traditional techniques prevail in the art system, computer art represents an experimental field in which the image is the product of mathematical formulas and algorithms. Artists, critics and art historians have described it as a new type of art, the latest encounter between humanistic and scientific culture. Moreover, since the computer is a scientific research machine – from astronomy to geometry, from medicine to physics – digital images stand between artistic invention and scientific evidence.

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