Identification of Expressed Sequence Markers for a Major Gene‐Rich Region of Wheat Chromosome Group 1 Using RNA Fingerprinting–Differential Display

Crop Science ◽  
2002 ◽  
Vol 42 (4) ◽  
pp. 1285-1290 ◽  
Author(s):  
Devinder Sandhu ◽  
Deepak Sidhu ◽  
Kulvinder S. Gill
Keyword(s):  
Differential Display ◽  
Major Gene ◽  
Wheat Chromosome ◽  
Rich Region ◽  
Chromosome Group ◽  
Gene Rich Region ◽  
Expressed Sequence ◽  
Rna Fingerprinting ◽  
Group 1

scholarly journals Identification and Physical Localization of Useful Genes and Markers to a Major Gene-Rich Region on Wheat Group1SChromosomes

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1735-1747 ◽  
Author(s):  
Devinder Sandhu ◽  
Julie A Champoux ◽  
Svetlana N Bondareva ◽  
Kulvinder S Gill
Keyword(s):  
Dna Analysis ◽  
Major Gene ◽  
Rflp Markers ◽  
Rich Region ◽  
Wheat Group ◽  
Marker Loci ◽  
Group 1 Chromosomes ◽  
Gene Rich Region ◽  
Homeologous Group ◽  
Group 1

AbstractThe short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. The objectives of this study were to physically localize gene-containing regions of the group 1 short arm, enrich these regions with markers, and study the distribution of genes and recombination. We focused on the major gene-rich region (“1S0.8 region”) and identified 75 useful genes along with 93 RFLP markers by comparing 35 different maps of Poaceae species. The RFLP markers were tested by gel blot DNA analysis of wheat group 1 nullisomic-tetrasomic lines, ditelosomic lines, and four single-break deletion lines for chromosome arm 1BS. Seventy-three of the 93 markers mapped to group 1 and detected 91 loci on chromosome 1B. Fifty-one of these markers mapped to two major gene-rich regions physically encompassing 14% of the short arm. Forty-one marker loci mapped to the 1S0.8 region and 10 to 1S0.5 region. Two cDNA markers mapped in the centromeric region and the remaining 24 loci were on the long arm. About 82% of short arm recombination was observed in the 1S0.8 region and 17% in the 1S0.5 region. Less than 1% recombination was observed for the remaining 85% of the physical arm length.

scholarly journals Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4

Genetics ◽  
2004 ◽  
Vol 168 (2) ◽  
pp. 651-663 ◽  
Author(s):  
Miftahudin ◽  
K. Ross ◽  
X.-F. Ma ◽  
A. A. Mahmoud ◽  
J. Layton ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Wheat Chromosome ◽  
Chromosome Group ◽  
Group 4 ◽  
Expressed Sequence

H9, H10, and H11 compose a cluster of Hessian fly-resistance genes in the distal gene-rich region of wheat chromosome 1AS

2005 ◽  
Vol 110 (8) ◽  
pp. 1473-1480 ◽  
Author(s):  
X. M. Liu ◽  
A. K. Fritz ◽  
J. C. Reese ◽  
G. E. Wilde ◽  
B. S. Gill ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Wheat Chromosome ◽  
Hessian Fly ◽  
Rich Region ◽  
Hessian Fly Resistance ◽  
Gene Rich Region ◽  
Distal Gene

Chromosomal localization of seven HSA3q13→q23 NotI linking clones on chicken microchromosomes: orthology of GGA14 and GGA15 to a gene-rich region of HSA3

2005 ◽  
Vol 111 (2) ◽  
pp. 128-133 ◽  
Author(s):  
A.A. Sazanov ◽  
A.L. Sazanova ◽  
V.A. Stekol’nikova ◽  
A.A. Kozyreva ◽  
M.N. Romanov ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Rich Region ◽  
Gene Rich Region

scholarly journals Differences in recombination frequencies during female and male meioses of the sex chromosomes of the medaka, Oryzias latipes

2001 ◽  
Vol 78 (1) ◽  
pp. 23-30 ◽  
Author(s):  
MARIKO KONDO ◽  
ERIKO NAGAO ◽  
HIROSHI MITANI ◽  
AKIHIRO SHIMA
Keyword(s):  
Genetic Map ◽  
Sex Chromosomes ◽  
Sex Chromosome ◽  
Oryzias Latipes ◽  
Male Recombination ◽  
Male And Female ◽  
Y Chromosomes ◽  
Genetic Length ◽  
Expressed Sequence ◽  
Group 1

In the medaka, Oryzias latipes, sex is determined chromosomally. The sex chromosomes differ from those of mammals in that the X and Y chromosomes are highly homologous. Using backcross panels for linkage analysis, we mapped 21 sequence tagged site (STS) markers on the sex chromosomes (linkage group 1). The genetic map of the sex chromosome was established using male and female meioses. The genetic length of the sex chromosome was shorter in male than in female meioses. The region where male recombination is suppressed is the region close to the sex-determining gene y, while female recombination was suppressed in both the telomeric regions. The restriction in recombination does not occur uniformly on the sex chromosome, as the genetic map distances of the markers are not proportional in male and female recombination. Thus, this observation seems to support the hypothesis that the heterogeneous sex chromosomes were derived from suppression of recombination between autosomal chromosomes. In two of the markers, Yc-2 and Casp6, which were expressed sequence-tagged (EST) sites, polymorphisms of both X and Y chromosomes were detected. The alleles of the X and Y chromosomes were also detected in O. curvinotus, a species related to the medaka. These markers could be used for genotyping the sex chromosomes in the medaka and other species, and could be used in other studies on sex chromosomes.

scholarly journals The highly recombinogenic bz locus lies in an unusually gene-rich region of the maize genome

2001 ◽  
Vol 98 (15) ◽  
pp. 8903-8908 ◽  
Author(s):  
H. Fu ◽  
W. Park ◽  
X. Yan ◽  
Z. Zheng ◽  
B. Shen ◽  
...  
Keyword(s):  
Maize Genome ◽  
Rich Region ◽  
Gene Rich Region

Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Stuart G. Gordon ◽  
Steven K. St. Martin ◽  
Anne E. Dorrance
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Rich Region ◽  
Molecular Linkage Group ◽  
Gene Rich Region

Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs

2007 ◽  
Vol 4 (1) ◽  
pp. 9-14
Author(s):  
Liu Yong-Gang ◽  
Xiong Yuan-Zhu ◽  
Zuo Bo ◽  
Jiang Si-Wen ◽  
Deng Chang-Yan ◽  
...  
Keyword(s):  
Expression Profile ◽  
Differential Display ◽  
Expressed Sequence Tags ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Large White ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Tissues ◽  
Expressed Sequence

AbstractIn order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.

Transcript Mapping of the Human Chromosome 11q12–q13.1 Gene-Rich Region Identifies Several Newly Described Conserved Genes

Genomics ◽  
1998 ◽  
Vol 49 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Paul R. Cooper ◽  
Norma J. Nowak ◽  
Michael J. Higgins ◽  
Deanna M. Church ◽  
Thomas B. Shows
Keyword(s):  
Human Chromosome ◽  
Transcript Mapping ◽  
Rich Region ◽  
Conserved Genes ◽  
Gene Rich Region
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