Nitrate-Induced Iron Deficiency in Soybean Varieties with Varying Iron-Stress Responses

2010 ◽  
Vol 102 (6) ◽  
pp. 1738-1744 ◽  
Author(s):  
John V. Wiersma
Keyword(s):  
Iron Deficiency ◽  
Stress Responses ◽  
Iron Stress

scholarly journals Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)

Plant Stress ◽  
2021 ◽  
pp. 100012
Author(s):  
Leorrie Atencio ◽  
Justin Salazar ◽  
Adrienne N. Moran Lauter ◽  
Michael D. Gonzales ◽  
Jamie A. O'Rourke ◽  
...  
Keyword(s):  
Glycine Max ◽  
Iron Deficiency ◽  
Stress Responses ◽  
Iron Stress ◽  
Glycine Max L ◽  
Short And Long Term

scholarly journals Examining Short-Term Responses to a Long-Term Problem: RNA-Seq Analyses of Iron Deficiency Chlorosis Tolerant Soybean

2020 ◽  
Vol 21 (10) ◽  
pp. 3591
Author(s):  
Adrienne N. Moran Lauter ◽  
Lindsay Rutter ◽  
Dianne Cook ◽  
Jamie A. O’Rourke ◽  
Michelle A. Graham
Keyword(s):  
Iron Deficiency ◽  
Stress Responses ◽  
Crop Production ◽  
Calcareous Soils ◽  
The United States ◽  
Iron Stress ◽  
Iron Deficiency Chlorosis ◽  
Genome Expression ◽  
Whole Genome Expression ◽  
Over Time

Iron deficiency chlorosis (IDC) is a global crop production problem, significantly impacting yield. However, most IDC studies have focused on model species, not agronomically important crops. Soybean is the second largest crop grown in the United States, yet the calcareous soils across most of the upper U.S. Midwest limit soybean growth and profitability. To understand early soybean iron stress responses, we conducted whole genome expression analyses (RNA-sequencing) of leaf and root tissue from the iron efficient soybean (Glycine max) cultivar Clark, at 30, 60 and 120 min after transfer to iron stress conditions. We identified over 10,000 differentially expressed genes (DEGs), with the number of DEGs increasing over time in leaves, but decreasing over time in roots. To investigate these responses, we clustered our expression data across time to identify suites of genes, their biological functions, and the transcription factors (TFs) that regulate their expression. These analyses reveal the hallmarks of the soybean iron stress response (iron uptake and homeostasis, defense, and DNA replication and methylation) can be detected within 30 min. Furthermore, they suggest root to shoot signaling initiates early iron stress responses representing a novel paradigm for crop stress adaptations.

scholarly journals A Mass Spectrometry-Based Study Shows that Volatiles Emitted by Arthrobacter agilis UMCV2 Increase the Content of Brassinosteroids in Medicago truncatula in Response to Iron Deficiency Stress

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 3011
Author(s):  
Idolina Flores-Cortez ◽  
Robert Winkler ◽  
Arturo Ramírez-Ordorica ◽  
Ma. Isabel Cristina Elizarraraz-Anaya ◽  
María Teresa Carrillo-Rayas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Iron Deficiency ◽  
Medicago Truncatula ◽  
Stress Responses ◽  
Crop Yields ◽  
Abiotic Factors ◽  
Defense Responses ◽  
Iron Deficient ◽  
Arthrobacter Agilis ◽  
Microbial Volatiles

Iron is an essential plant micronutrient. It is a component of numerous proteins and participates in cell redox reactions; iron deficiency results in a reduction in nutritional quality and crop yields. Volatiles from the rhizobacterium Arthrobacter agilis UMCV2 induce iron acquisition mechanisms in plants. However, it is not known whether microbial volatiles modulate other metabolic plant stress responses to reduce the negative effect of iron deficiency. Mass spectrometry has great potential to analyze metabolite alterations in plants exposed to biotic and abiotic factors. Direct liquid introduction-electrospray-mass spectrometry was used to study the metabolite profile in Medicago truncatula due to iron deficiency, and in response to microbial volatiles. The putatively identified compounds belonged to different classes, including pigments, terpenes, flavonoids, and brassinosteroids, which have been associated with defense responses against abiotic stress. Notably, the levels of these compounds increased in the presence of the rhizobacterium. In particular, the analysis of brassinolide by gas chromatography in tandem with mass spectrometry showed that the phytohormone increased ten times in plants grown under iron-deficient growth conditions and exposed to microbial volatiles. In this mass spectrometry-based study, we provide new evidence on the role of A. agilis UMCV2 in the modulation of certain compounds involved in stress tolerance in M. truncatula.

scholarly journals Transcriptome Analysis Reveals IsiA-Regulatory Mechanisms Underlying Iron Depletion and Oxidative-Stress Acclimation in Synechocystis sp. Strain PCC 6803

2020 ◽  
Vol 86 (13) ◽  
Author(s):  
Yarui Cheng ◽  
Tianyuan Zhang ◽  
Li Wang ◽  
Wenli Chen
Keyword(s):  
Oxidative Stress ◽  
Network Analysis ◽  
Iron Deficiency ◽  
Regulatory Mechanisms ◽  
Functional Modules ◽  
Iron Stress ◽  
Iron Starvation ◽  
Stress Acclimation ◽  
And Oxidative Stress ◽  
Coexpression Network Analysis

ABSTRACT Microorganisms in nature are commonly exposed to various stresses in parallel. The isiA gene encodes an iron stress-induced chlorophyll-binding protein which is significantly induced under iron starvation and oxidative stress. Acclimation of oxidative stress and iron deficiency was investigated using a regulatory mutant of the Synechocystis sp. strain PCC 6803. In this study, the ΔisiA mutant grew more slowly in oxidative-stress and iron depletion conditions compared to the wild-type (WT) counterpart under the same conditions. Thus, we performed transcriptome sequencing (RNA-seq) analysis of the WT strain and the ΔisiA mutant under double-stress conditions to obtain a comprehensive view of isiA-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed significant differences between the WT strain and ΔisiA mutant, mainly related to photosynthesis and the iron-sulfur cluster. The deletion of isiA affects the expression of various genes that are involved in cellular processes and structures, such as photosynthesis, phycobilisome, and the proton-transporting ATPase complex. Weighted gene coexpression network analysis (WGCNA) demonstrated three functional modules in which the turquoise module was negatively correlated with superoxide dismutase (SOD) activity. Coexpression network analysis identified several hub genes of each module. Cotranscriptional PCR and reads coverage using the Integrative Genomics Viewer demonstrated that isiA, isiB, isiC, ssl0461, and dfp belonged to the isi operon. Three sRNAs related to oxidative stress were identified. This study enriches our knowledge of IsiA-regulatory mechanisms under iron deficiency and oxidative stress. IMPORTANCE This study analyzed the impact of isiA deletion on the transcriptomic profile of Synechocystis. The isiA gene encodes an iron stress-induced chlorophyll-binding protein, which is significantly induced under iron starvation. The deletion of isiA affects the expression of various genes that are involved in photosynthesis and ABC transporters. WGCNA revealed three functional modules in which the blue module was correlated with oxidative stress. We further demonstrated that the isi operon contained the following five genes: isiA, isiB, isiC, ssl0461, and dfp by cotranscriptional PCR. Three sRNAs were identified that were related to oxidative stress. This study enhances our knowledge of IsiA-regulatory mechanisms under iron deficiency and oxidative stress.

scholarly journals Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

2021 ◽  
Vol 22 (20) ◽  
pp. 11032
Author(s):  
Jamie A. O’Rourke ◽  
Michael J. Morrisey ◽  
Ryan Merry ◽  
Mary Jane Espina ◽  
Aaron J. Lorenz ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Iron Deficiency ◽  
Gene Silencing ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Yield Loss ◽  
Abiotic Stress Tolerance ◽  
Iron Stress ◽  
Rna Seq ◽  
Virus Induced Gene Silencing

The soybean (Glycine max L. merr) genotype Fiskeby III is highly resistant to a multitude of abiotic stresses, including iron deficiency, incurring only mild yield loss during stress conditions. Conversely, Mandarin (Ottawa) is highly susceptible to disease and suffers severe phenotypic damage and yield loss when exposed to abiotic stresses such as iron deficiency, a major challenge to soybean production in the northern Midwestern United States. Using RNA-seq, we characterize the transcriptional response to iron deficiency in both Fiskeby III and Mandarin (Ottawa) to better understand abiotic stress tolerance. Previous work by our group identified a quantitative trait locus (QTL) on chromosome 5 associated with Fiskeby III iron efficiency, indicating Fiskeby III utilizes iron deficiency stress mechanisms not previously characterized in soybean. We targeted 10 of the potential candidate genes in the Williams 82 genome sequence associated with the QTL using virus-induced gene silencing. Coupling virus-induced gene silencing with RNA-seq, we identified a single high priority candidate gene with a significant impact on iron deficiency response pathways. Characterization of the Fiskeby III responses to iron stress and the genes underlying the chromosome 5 QTL provides novel targets for improved abiotic stress tolerance in soybean.

Bicarbonate blocks iron translocation from cotyledons inducing iron stress responses in Citrus roots

2013 ◽  
Vol 170 (10) ◽  
pp. 899-905 ◽  
Author(s):  
Mary-Rus Martínez-Cuenca ◽  
Francisco Legaz ◽  
M. Ángeles Forner-Giner ◽  
Eduardo Primo-Millo ◽  
Domingo J. Iglesias
Keyword(s):  
Stress Responses ◽  
Iron Stress
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