Transporter mRNA Expression in a Conditionally Immortalized Rat Small Intestine Epithelial Cell Line (TR-SIE)

2004 ◽  
Vol 19 (4) ◽  
pp. 264-269 ◽  
Author(s):  
Ken-ichi Hosoya ◽  
Masatoshi Tomi ◽  
Megumi Takayama ◽  
Yuko Komokata ◽  
Daisuke Nakai ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Rat Small Intestine

scholarly journals Differentiation of intestinal epithelial cell line (IEC-18) by an acid extract of rat small intestine

FEBS Letters ◽  
1989 ◽  
Vol 255 (2) ◽  
pp. 423-426 ◽  
Author(s):  
Takafumi Shintani ◽  
Tohru Fushiki ◽  
Shin-ichi Fukuoka ◽  
Hiromi Takahashi-Iwanaga ◽  
Etsuro Sugimoto
Keyword(s):  
Small Intestine ◽  
Epithelial Cell ◽  
Cell Line ◽  
Intestinal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Acid Extract ◽  
Rat Small Intestine ◽  
Intestinal Epithelial Cell Line

scholarly journals Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line

2013 ◽  
Vol 79 (7) ◽  
pp. 2225-2232 ◽  
Author(s):  
Murphy Lam-Yim Wan ◽  
Chit-Shing Jackson Woo ◽  
Kevin J. Allen ◽  
Paul C. Turner ◽  
Hani El-Nezami
Keyword(s):  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Defense Mechanisms ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epithelial Cell Line ◽  
Content Type ◽  
Antimicrobial Effects ◽  
Food And Feed

ABSTRACTDefensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four commonFusariumtoxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA), and fumonisin B1 (FB1), on the mRNA expression, protein secretion, and corresponding antimicrobial effects of porcine β-defensins 1 and 2 (pBD-1 and pBD-2) using a porcine jejunal epithelial cell line, IPEC-J2. In general, upregulation of pBD-1 and pBD-2 mRNA expression occurred following exposure toFusariumtoxins, individually and in mixtures (P< 0.05). However, no significant increase in secreted pBD-1 and pBD-2 protein levels was observed, as measured by enzyme-linked immunosorbent assay (ELISA). Supernatants from IPEC-J2 cells exposed to toxins, singly or in combination, however, possessed significantly less antimicrobial activity againstEscherichia colithan untreated supernatants. When single toxins and two-toxin combinations were assessed, toxicity effects were shown to be nonadditive (including synergism, potentiation, and antagonism), suggesting interactive toxin effects when cells are exposed to mycotoxin combinations. The results show thatFusariumtoxins, individually and in mixtures, activate distinct antimicrobial defense mechanisms possessing the potential to alter the intestinal microbiota through diminished antimicrobial effects. Moreover, by evaluating toxin mixtures, this improved understanding of toxin effects will enable more effective risk assessments for common mycotoxin combinations observed in contaminated food and feed.

Zinc transporter mRNA expression in the RWPE-1 human prostate epithelial cell line

BioMetals ◽  
2007 ◽  
Vol 21 (4) ◽  
pp. 405-416 ◽  
Author(s):  
Amy L. Albrecht ◽  
Seema Somji ◽  
Mary Ann Sens ◽  
Donald A. Sens ◽  
Scott H. Garrett
Keyword(s):  
Epithelial Cell ◽  
Mrna Expression ◽  
Cell Line ◽  
Zinc Transporter ◽  
Human Prostate ◽  
Epithelial Cell Line ◽  
Prostate Epithelial Cell ◽  
Human Prostate Epithelial Cell
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