scholarly journals DNA MICROARRAY ANALYSIS OF TRANSCRIPTIONAL RESPONSES OF YEAST CELLS TO EXPRESSION OF CORE PROTEIN OF HEPATITIS C VIRUS

2007 ◽  
Vol 32 (2) ◽  
pp. 201-204 ◽  
Author(s):  
Naoko KUBOTA ◽  
Akira NAGANUMA ◽  
Shusuke KUGE
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Core Protein ◽  
Yeast Cells ◽  
Transcriptional Responses ◽  
Dna Microarray Analysis

scholarly journals The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation

2004 ◽  
Vol 85 (4) ◽  
pp. 971-981 ◽  
Author(s):  
Nathalie Majeau ◽  
Valérie Gagné ◽  
Annie Boivin ◽  
Marilène Bolduc ◽  
Josée-Anne Majeau ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Host Cells ◽  
Yeast Cells ◽  
Main Function ◽  
C Protein ◽  
Multifunctional Protein ◽  
The Core

The core (C) protein of hepatitis C virus (HCV) appears to be a multifunctional protein that is involved in many viral and cellular processes. Although its effects on host cells have been extensively discussed in the literature, little is known about its main function, the assembly and packaging of the viral genome. We have studied the in vitro assembly of several deleted versions of recombinant HCV C protein expressed in E. coli. We demonstrated that the 75 N-terminal residues of the C protein were sufficient to assemble and generate nucleocapsid-like particles (NLPs) in vitro. However, homogeneous particles of regular size and shape were observed only when NLPs were produced from at least the first 79 N-terminal amino acids of the C protein. This small protein unit fused to the endoplasmic reticulum-anchoring domain also generated NLPs in yeast cells. These data suggest that the N-terminal half of the C protein is important for formation of NLPs. Similarities between the HCV C protein and C proteins of other members of the Flaviviridae are discussed.

scholarly journals DNA microarray analysis of transcriptional responses of rats housed on solid and grid cage flooring

2012 ◽  
Vol 37 (1) ◽  
pp. 219-222
Author(s):  
Masayuki Uehara ◽  
Ayako Nishio ◽  
Satoshi Inoue ◽  
Kiyoshi Matsumoto
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Transcriptional Responses ◽  
Dna Microarray Analysis

scholarly journals DNA microarray analysis of transcriptional responses of mouse spinal cords to physical exercise

2009 ◽  
Vol 34 (4) ◽  
pp. 445-448 ◽  
Author(s):  
Kazunori Hashimoto ◽  
Akiko Honda ◽  
Yuichi Hayashi ◽  
Takashi Inuzuka ◽  
Masahiko Satoh ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Transcriptional Responses ◽  
Dna Microarray Analysis

scholarly journals HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells

FEBS Letters ◽  
2012 ◽  
Vol 586 (16) ◽  
pp. 2318-2325 ◽  
Author(s):  
Naoko Kubota ◽  
Yasutaka Inayoshi ◽  
Naoko Satoh ◽  
Takashi Fukuda ◽  
Kenta Iwai ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein Stability ◽  
Core Protein ◽  
Yeast Cells ◽  
Virus Core

Biosynthesis and biochemical properties of the hepatitis C virus core protein.

1994 ◽  
Vol 68 (6) ◽  
pp. 3631-3641 ◽  
Author(s):  
E Santolini ◽  
G Migliaccio ◽  
N La Monica
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Biochemical Properties ◽  
Virus Core

Truncated hepatitis C virus core protein encoded in hepatocellular carcinomas

2004 ◽  
Author(s):  
Rin Yamaguchi ◽  
Seiya Momosaki ◽  
Guang Gao ◽  
Chu Hsia ◽  
Masamichi Kojiro ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Virus Core ◽  
Hepatocellular Carcinomas

scholarly journals The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 60
Author(s):  
Hisae Aoshima ◽  
Masayuki Ito ◽  
Rinta Ibuki ◽  
Hirokazu Kawagishi
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Cell Activation ◽  
Skin Barrier ◽  
Efficacy Evaluation ◽  
Activation Effect ◽  
Expression Levels ◽  
Dna Microarray Analysis

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

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