ANALYSIS OF THE EXPRESSION OF HEME OXYGENASE-1 GENE IN HUMAN ALVEOLAR EPITHELIAL CELLS EXPOSED TO CIGARETTE SMOKE CONDENSATE

2006 ◽  
Vol 31 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Yasuo FUKANO ◽  
Masako OISHI ◽  
Fumika CHIBANA ◽  
Satoshi NUMAZAWA ◽  
Takemi YOSHIDA
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Heme Oxygenase ◽  
Alveolar Epithelial Cells ◽  
Heme Oxygenase 1 ◽  
Cigarette Smoke Condensate ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cells

Cigarette smoke-induced kinin B1 receptor promotes NADPH oxidase activity in cultured human alveolar epithelial cells

Peptides ◽  
2011 ◽  
Vol 32 (7) ◽  
pp. 1447-1456 ◽  
Author(s):  
Sébastien Talbot ◽  
James Chi-Jen Lin ◽  
Karim Lahjouji ◽  
Jean-Philippe Roy ◽  
Jacques Sénécal ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Nadph Oxidase ◽  
Cigarette Smoke ◽  
Oxidase Activity ◽  
Alveolar Epithelial Cells ◽  
Nadph Oxidase Activity ◽  
Alveolar Epithelial ◽  
B1 Receptor ◽  
Kinin B1 Receptor ◽  
Human Alveolar Epithelial Cells

scholarly journals Induction of γ-glutamylcysteine synthetase by cigarette smoke is associated with AP-1 in human alveolar epithelial cells

FEBS Letters ◽  
1996 ◽  
Vol 396 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Irfan Rahman ◽  
Christopher A.D. Smith ◽  
Mark F. Lawson ◽  
David J. Harrison ◽  
William MacNee
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Glutamylcysteine Synthetase ◽  
Human Alveolar Epithelial Cells

Differential regulation of glutathione by oxidants and dexamethasone in alveolar epithelial cells

1998 ◽  
Vol 275 (1) ◽  
pp. L80-L86 ◽  
Author(s):  
Irfan Rahman ◽  
Agnes Bel ◽  
Brigitte Mulier ◽  
Kenneth Donaldson ◽  
William MacNee
Keyword(s):  
Epithelial Cells ◽  
Xanthine Oxidase ◽  
Cigarette Smoke ◽  
Control Level ◽  
Alveolar Epithelial Cells ◽  
Time Dependent ◽  
Cigarette Smoke Condensate ◽  
Alveolar Epithelial ◽  
Glutamylcysteine Synthetase ◽  
Glutamyl Transpeptidase

We studied the regulation of GSH and the enzymes involved in GSH regulation, γ-glutamylcysteine synthetase (γ-GCS) and γ-glutamyl transpeptidase (γ-GT), in response to the oxidants menadione, xanthine/xanthine oxidase, hyperoxia, and cigarette smoke condensate in human alveolar epithelial cells (A549). Menadione (100 μM), xanthine/xanthine oxidase (50 μM/10 mU), and cigarette smoke condensate (10%) exposure produced increased GSH levels (240 ± 6, 202 ± 12, and 191 ± 2 nmol/mg protein, respectively; P < 0.001) compared with the control level (132 ± 8 nmol/mg protein), which were associated with a significant increase in γ-GCS activity (0.18 ± 0.006, 0.16 ± 0.01, and 0.17 ± 0.008 U/mg protein, respectively; P < 0.01) compared with the control level (0.08 ± 0.001 U/mg protein) at 24 h. Exposure to hyperoxia (95% O2) resulted in a time-dependent increase in GSH levels. γ-GCS activity increased significantly at 4 h ( P < 0.001), returning to control values after 12 h of exposure. Dexamethasone (3 μM) exposure produced a significant time-dependent decrease in the levels of GSH and γ-GCS activity at 24–96 h. The activity of γ-GT did not change after oxidant treatment; however, it was decreased significantly by dexamethasone at 24–96 h. Thus oxidants and dexamethasone modulate GSH levels and activities of γ-GT and γ-GCS by different mechanisms. We suggest that the increase in γ-GCS activity but not in γ-GT activity may be required for the increase in intracellular GSH under oxidative stress in alveolar epithelial cells.

Sign in / Sign up

Export Citation Format

Share Document