scholarly journals Subacute toxicity of (-)15-deoxyspergualin in BALB/c mice. I. Hematological study.

1989 ◽  
Vol 14 (4) ◽  
pp. 237-245 ◽  
Author(s):  
Michihito OKUBO ◽  
Yoshihiko MASAKI ◽  
Keiichi INOUE ◽  
Rieko HARA ◽  
Miyako TAIRA ◽  
...  
Keyword(s):  
Subacute Toxicity

Safety Evaluation of Eucalyptus globulus Essential Oils through Acute and Sub-acute Toxicity and Skin Irritation in Mice and Rats

2020 ◽  
Vol 14 (3) ◽  
pp. 187-195
Author(s):  
Berhan Mengiste ◽  
Tizazu Zenebe ◽  
Kassahun Dires ◽  
Ermias Lulekal ◽  
Awol Mekonnen ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oil ◽  
Eucalyptus Globulus ◽  
Biochemical Parameters ◽  
Skin Irritation ◽  
Toxicity Study ◽  
Subacute Toxicity ◽  
Acute Toxicity Study ◽  
Limit Test ◽  
Ointment Formulation

Background: The Eucalyptus globulus extractions have been used by the traditional healers to treat diseases in the study area. Our previous study revealed that the essential oil has antimicrobial and antifungal activity. This study determined phytochemical analysis, skin irritation, acute and subacute toxicity of Eucalyptus globulus essential oil in mice and rats. Methods: The phytochemicals were analyzed using GC-MS mass spectrometry. The acute toxicity study was determined at three dose levels of 1500 mg/kg, 1750mg/kg, and 2000 mg/kg. The essential oil limit test at a dose of 1000 mg/kg was administered to mice for 28 consecutive days for sub-acute toxicity study. The mice mortality, behavioral change, injury and other signs of illness were recorded once daily. Biochemical parameters were evaluated. Liver and kidney were analyzed for histopathological analyses. The 5% ointment formulation was applied to the rat skin to determine skin irritation effects. Results: The Eucalyptus globulus essential oil showed no effect on the mice at a dose of 1500mg/kg and below, but caused signs of toxicity and death at a dose of 1750mg/kg and above compared to the controls (p<0.05). The LD50 value was 1650 mg/kg. There was no significant difference (p > 0.05) in the body weights, gross abnormalities of the organs and biochemical parameters compared to the control at 1000 mg/kg subacute toxicity study. No histopathological changes were detected in the organs tested. The 5% ointment formulation did not show any abnormal skin reaction. Discussion: In the present study, the Eucalyptus globulus essential oil was comparable with other studies in terms of both chemical composition and its effects on sub-acute and topical application. Conclusion: This toxicity study demonstrated that Eucalyptus globulus essential oil is nontoxic at a relatively lower concentration.

scholarly journals Toxicological evaluation of aqueous extract of the traditional Chinese formula Qing Hao Gan Cao

2021 ◽  
Author(s):  
Yongchun Li ◽  
Hui Zhang ◽  
Shanshan Chen ◽  
Liutao Zhao ◽  
Jie Wu ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Toxicity Test ◽  
Artemisia Annua ◽  
Hematological Parameters ◽  
Organ Weight ◽  
Toxicological Evaluation ◽  
Subacute Toxicity ◽  
Qing Hao

Abstract Qing Hao Gan Cao (QHGC), a Chinese medicinal formula containing Artemisia annua and Glycyrrhizae Radix et Rhizoma, has been used to treat sunstroke and as an antiviral agent for more than 800 years. It has not previously been subject to a toxicological safety evaluation in acute and subacute (28 days) studies. Therefore, the acute and subacute toxicity of an aqueous extract of QHGC were evaluated in vivo. For the QHGC preparation, the botanical raw materials were crushed into pieces and mixed in the ratio of 10:1 in distilled water for 12 h, then boiling three times for 2 h each time. The three decoctions were mixed and filtered, then spray-dried with hot air at 160°C for 30 min, and stored at room temperature. For the acute toxicity test, 72.0 g/kg of QHGC extract was administered by gavage to male and female mice. Body weight, general observations, and autopsy results were recorded. No mortality or toxicity signs were observed during the studies. For the subacute toxicity test, 4.0, 8.0, or 16.0 g/kg/day of QHGC extract was administered to rats for 28 days. General observations and mortality, body weight, biochemical and hematological parameters, organ weight, and pathological morphology were analyzed. The acute and subacute toxicity studies did not show significant changes in body weight, general observations, hematology and biochemical parameters, organ weight, and liver, spleen, stomach, duodenum, testis, ovary, lung, heart, and kidney histopathological analyses. The consumption of QHGC aqueous extract can be considered safe within the conditions of this study.

scholarly journals In Vivo Subacute Toxicity and Antidiabetic Effect of Aqueous Extract of Nigella sativa

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Karima Bensiameur-Touati ◽  
Ghouti Kacimi ◽  
El-Mehdi Haffaf ◽  
Sihem Berdja ◽  
Souhila Aouichat-Bouguerra
Keyword(s):  
Aqueous Extract ◽  
Nigella Sativa ◽  
Histological Study ◽  
Diabetic Rats ◽  
Antidiabetic Effect ◽  
Subacute Toxicity ◽  
Wide Range ◽  
Liver And Pancreas ◽  
Liver Tbars

Context. Nigella sativa seeds are usually used as traditional medicine for a wide range of therapeutic purposes. Objective. To investigate the subacute toxicity of NS aqueous extract and select its lowest dose to study its antidiabetic effect. Methods. 5 AqE.NS doses (2, 6.4, 21, 33, and 60 g/Kg) were daily administered to mice by gavage. Biochemical parameters measurements and histological study of the liver and the kidney were performed after 6 weeks of supplementation. Thereafter, and after inducing diabetes by alloxan, rats were treated by 2 g/Kg of AqE.NS during 8 weeks. Metabolic parameters were measured on sera. A horizontal electrophoresis of plasmatic lipoprotein was conducted. Glycogen, total lipids, and triglycerides were measured in the liver. TBARS were evaluated on adipose tissue, liver, and pancreas. Results. AqE.NS showed no variation in urea and albumin at the 5 doses, but hepatotoxicity from 21 g/Kg was confirmed by histopathological observations of the liver. In diabetic rats, AqE.NS significantly decreased glycemia, TG, T-cholesterol, LDL-c, and TBARS and showed a restored insulinemia and a significant increase in HDL-c. Results on the liver indicated a decrease in lipids and a possible glycogenogenesis. Conclusion. AqE.NS showed its safety at low doses and its evident antihyperglycemic, antihyperlipidemic, and antioxidant effect.

scholarly journals Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Alian Désiré Afagnigni ◽  
Maximilienne Ascension Nyegue ◽  
Chantal Florentine Ndoye Foe ◽  
Youchahou Njankouo Ndam ◽  
Frédéric Nico Njayou ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Female Rats ◽  
Male Rats ◽  
Dependent Manner ◽  
Subacute Toxicity ◽  
Antidiarrheal Activity ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

Subacute Toxicity of Cyclopiazonic Acid in Broiler Chicks Fed Normal and High Zinc Diets

1990 ◽  
pp. 151-160 ◽  
Author(s):  
Mark E. Wilson ◽  
Winston M. Hagler ◽  
Jon F. Ort ◽  
John M. Cullen ◽  
Richard J. Cole
Keyword(s):  
Cyclopiazonic Acid ◽  
Broiler Chicks ◽  
Subacute Toxicity ◽  
High Zinc

scholarly journals Acute and Subacute Toxicity Evaluation of Corn Silk Extract

2018 ◽  
Vol 23 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Ae Wha Ha ◽  
Hyeon Jung Kang ◽  
Sun Lim Kim ◽  
Myung Hwan Kim ◽  
Woo Kyoung Kim
Keyword(s):  
Toxicity Evaluation ◽  
Corn Silk ◽  
Subacute Toxicity

Acute and subacute toxicity studies of Aegle marmelos Corr., an Indian medicinal plant

Phytomedicine ◽  
2007 ◽  
Vol 14 (2-3) ◽  
pp. 209-215 ◽  
Author(s):  
A. Veerappan ◽  
S. Miyazaki ◽  
M. Kadarkaraisamy ◽  
D. Ranganathan
Keyword(s):  
Medicinal Plant ◽  
Aegle Marmelos ◽  
Indian Medicinal Plant ◽  
Subacute Toxicity ◽  
Toxicity Studies
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