scholarly journals Lin28 overexpression inhibits neurite outgrowth of primary cortical neurons in vitro

2018 ◽  
Vol 78 (4) ◽  
pp. 297-304
Author(s):  
Mohammad Iqbal Hossain Bhuiyan ◽  
Seong Yun Kim ◽  
Kyung-Ok Cho
Keyword(s):  
Neurite Outgrowth ◽  
Cortical Neurons ◽  
Primary Cortical Neurons

scholarly journals A High-throughput Assay for mRNA Silencing in Primary Cortical Neurons in vitro with Oligonucleotide Therapeutics

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (16) ◽  
Author(s):  
Julia Alterman ◽  
Andrew Coles ◽  
Lauren Hall ◽  
Neil Aronin ◽  
Anastasia Khvorova ◽  
...  
Keyword(s):  
High Throughput ◽  
Cortical Neurons ◽  
Primary Cortical Neurons ◽  
High Throughput Assay

scholarly journals Low-level laser therapy (810 nm) protects primary cortical neurons against excitotoxicity in vitro

2013 ◽  
Vol 7 (8) ◽  
pp. 656-664 ◽  
Author(s):  
Ying-Ying Huang ◽  
Kazuya Nagata ◽  
Clark E Tedford ◽  
Michael R. Hamblin
Keyword(s):  
Laser Therapy ◽  
Cortical Neurons ◽  
Low Level Laser Therapy ◽  
Primary Cortical Neurons ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Population PKPD Modeling of BACE1 Inhibitor-Induced Reduction in Aβ Levels In Vivo and Correlation to In Vitro Potency in Primary Cortical Neurons from Mouse and Guinea Pig

2013 ◽  
Vol 31 (3) ◽  
pp. 670-683 ◽  
Author(s):  
Juliette Janson ◽  
Susanna Eketjäll ◽  
Karin Tunblad ◽  
Fredrik Jeppsson ◽  
Stefan Von Berg ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cortical Neurons ◽  
Primary Cortical Neurons ◽  
Bace1 Inhibitor ◽  
Pkpd Modeling ◽  
Population Pkpd

scholarly journals Coupling (reduced) Graphene Oxide to Mammalian Primary Cortical Neurons In Vitro

2015 ◽  
Vol 2 (3) ◽  
pp. 217-229 ◽  
Author(s):  
Antonina M. Monaco ◽  
◽  
Anastasiya Moskalyuk ◽  
Jaroslaw Motylewski ◽  
Farnoosh Vahidpour ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cortical Neurons ◽  
Primary Cortical Neurons ◽  
Reduced Graphene

scholarly journals Leptomeningeal Cells Induce Synaptic Failure via Cathepsin B-mediated IL-1ß Production after Porphyromonas Gingivalis Infection

2020 ◽  
Author(s):  
Wanyi Huang ◽  
Fan Zeng ◽  
Yebo Gu ◽  
Muzhou Jiang ◽  
Xinwen Zhang ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Cortical Neurons ◽  
Cathepsin B ◽  
Inflammasome Activation ◽  
Primary Cortical Neurons ◽  
Memory Decline ◽  
Protein Levels ◽  
Synaptic Failure ◽  
Bdnf Signaling

Abstract Background: Synaptic failure is the earliest sign before Alzheimer’s disease (AD) onset and closely associated with cognitive decline. Clinical studies have shown that periodontitis is positively correlated with both the onset and pathological progression of AD, and preclinical studies have shown that Porphyromonas gingivalis (P. gingivalis), the key pathogen in periodontitis, and its virulence factors induced memory decline in mice. However, the mechanisms underlying the involvement of P. gingivalis in memory decline remain unclear. Methods: We used primary leptomeningeal cells and primary cortical neurons to evaluate the effects of leptomeningeal cells on synaptic generation and plasticity after P. gingivalis infection in vitro. The expression of the related molecules was examined by mRNA in real time and by protein levels using Western blotting. Pharmacological and genetic approaches were used to explore the mechanism underlying the involvement of leptomeningeal cells in synaptic changes after P. gingivalis infection. Results: NLRP3 inflammasome activation was involved in augmenting the IL-1β secretion by primary leptomeningeal cells after P. gingivalis infection, as determined by the knockdown of NLRP3 with siRNA. Cathepsin B (CatB) mediated the activation of both NLRP3 inflammasomes and NF-κB for the production of IL-1β by P. gingivalis-infected primary leptomeningeal cells, as determined by the pharmacologically specific inhibition of CatB. In contrast, P. gingivalis-infected leptomeningeal cells induced an IL-1β-dependent decrease in pre- and post-synaptic molecules in primary cortical neurons, as determined by the pharmacological blockage of the IL-1 receptor. P. gingivalis-infected leptomeningeal cells also induced the IL-1β-dependent suppression of BDNF signaling in cultured N2a neurons, a stable mouse neural cell line. Furthermore, propolis, which is produced by honeybees, suppressed the expression of IL-1β but increased that of BDNF in P. gingivalis-infected leptomeningeal cells. Conclusion: The CatB-mediated IL-1β production was augmented in leptomeningeal cells, resulting in synaptic failure and blockage of BDNF signaling in neurons during P. gingivalis infection. These findings highlight a new mechanism underlying the involvement of periodontitis in AD initiation and suggest that CatB may be an early intervention therapeutic target for delaying the onset of AD during P. gingivalis infection.

Sonic Hedgehog Promotes Neurite Outgrowth of Primary Cortical Neurons Through Up-Regulating BDNF Expression

2015 ◽  
Vol 41 (4) ◽  
pp. 687-695 ◽  
Author(s):  
Weiliang He ◽  
Lili Cui ◽  
Cong Zhang ◽  
Xiangjian Zhang ◽  
Junna He ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Sonic Hedgehog ◽  
Cortical Neurons ◽  
Bdnf Expression ◽  
Primary Cortical Neurons

Noradrenaline acting on astrocytic β2-adrenoceptors induces neurite outgrowth in primary cortical neurons

2014 ◽  
Vol 77 ◽  
pp. 234-248 ◽  
Author(s):  
Jennifer S. Day ◽  
Eimear O'Neill ◽  
Caroline Cawley ◽  
Nicholas Kruseman Aretz ◽  
Dana Kilroy ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Cortical Neurons ◽  
Primary Cortical Neurons
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