scholarly journals Using Isothiazolone Biocides to Control Microbial and Fungal Contaminants in Plant Tissue Cultures

1998 ◽  
Vol 8 (4) ◽  
pp. 598-601 ◽  
Author(s):  
Randall P. Niedz
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Tissue ◽  
Culture Medium ◽  
Tissue Cultures ◽  
Fungal Contamination ◽  
Plant Tissue Cultures ◽  
Resistant Strains ◽  
Microbial Contaminants ◽  
Fungal Contaminants

Controlling bacterial and fungal contamination in plant tissue cultures is a serious problem. Antibiotics are currently used but are not always effective, can alter plant growth, and are costly, and resistant strains can result with extensive use. Plant preservative mixture (PPM) contains a mixture of two isothiazolones—methylchloroisothiazolinone and methylisothiazolinone, which are a class of broad-spectrum, widely used industrial biocides. The isothiazolones used in PPM are reported by the manufacturer to be nonphytotoxic at concentrations suitable for the prophylactic control of microbial contaminants in plant tissue cultures. Our results indicate that PPM can be routinely added to tissue culture medium to control air- and waterborne bacterial and fungal contaminants effectively.

Polysccharides of plant tissue cultures. II. Acid hydrolysis of wastes from a ginseng tissue culture

1988 ◽  
Vol 24 (3) ◽  
pp. 289-293
Author(s):  
E. P. Kukhta ◽  
I. V. Aleksandrova ◽  
S. P. Afanas'ev ◽  
V. N. Paukov ◽  
M. A. Lyal'chenko
Keyword(s):  
Tissue Culture ◽  
Acid Hydrolysis ◽  
Plant Tissue ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
Hydrolysis Of

scholarly journals Hyaluronic Acid Can be a New Plant Growth Regulator for Hybrid Cymbidium Micropropagation

2012 ◽  
Vol 22 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Naruemol Kaewjampa ◽  
Kazuhiko Shimasaki ◽  
Syeda Jabun Nahar
Keyword(s):  
Tissue Culture ◽  
Hyaluronic Acid ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Growth Regulator ◽  
Culture Medium ◽  
Fresh Weight ◽  
Maximum Percentage

Present authors investigated the effect of two biopolymer hyaluronic acid (HA9) and chitosan (Chitosan H), and N6-benzyladenine (BA) supplemented with modified MS on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium cultivar. The percentages of PLB formation from cultures showed 100 % in all treatments except for control (82.7%), 0.1 mg/l HA9 (93.3%) and 10 mg/l Chitosan H (93.3%) treatments. Addition of 0.1 mg/l HA9 to culture medium was highly efficient for PLB multiplication. Both 10 mg/l HA9 and 1.0 mg/l BA significantly increased the number of shoots in cultures. The maximum percentage of shoot (100%) was obtained at 1.0 mg/l chitosan H. Increase in fresh weight showed higher values at BA treatments. Information gained from this study shows that hyaluronic acid (HA9) which is new additive for plant tissue culture act as growth regulator for hybrid Cymbidium micropropagation. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11261Plant Tissue Cult. & Biotech. 22(1): 59-64, 2012 (June)

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

Cold acclimation of plant tissue cultures

Cryobiology ◽  
1971 ◽  
Vol 8 (4) ◽  
pp. 386-387 ◽  
Author(s):  
Peter L. Steponkus ◽  
L. Bannier
Keyword(s):  
Cold Acclimation ◽  
Plant Tissue ◽  
Tissue Cultures ◽  
Plant Tissue Cultures

Iron Nutrition for Growth and Chlorophyll Development of some Plant Tissue Cultures

Nature ◽  
1964 ◽  
Vol 202 (4938) ◽  
pp. 1235-1236 ◽  
Author(s):  
J. C. WILMAR ◽  
A. C. HILDEBRANDT ◽  
A. J. RIKER
Keyword(s):  
Plant Tissue ◽  
Iron Nutrition ◽  
Tissue Cultures ◽  
Plant Tissue Cultures

scholarly journals Osmotic Shrinkage as a Factor in Freezing Injury in Plant Tissue Cultures

1976 ◽  
Vol 57 (2) ◽  
pp. 290-296 ◽  
Author(s):  
Leigh E. Towill ◽  
Peter Mazur
Keyword(s):  
Plant Tissue ◽  
Freezing Injury ◽  
Tissue Cultures ◽  
Plant Tissue Cultures
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