scholarly journals A Rapid Nondestructive Technique to Predict Leaf Nitrogen Status of Grapefruit Tree with Various Nitrogen Fertilization Practices

1998 ◽  
Vol 8 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Y.C. Li ◽  
A.K. Alva ◽  
D.V. Calvert ◽  
M. Zhang

The objective of this study was to evaluate the use of a leaf chlorophyll meter (SPAD-502) to predict nitrogen status and yield response of grapefruit trees. The study included two irrigation treatments, three sources, and four rates of fertilizer [dry soluble granular fertilizer broadcast or through fertigation with N rates either 50, 100, 150 lb/acre/yr (56, 112, or 168 kg.ha-1.yr-1)], and controlled-release fertilizer with N rates either 25, 50, 100 lb/acre. Irrigation treatments did not affect SPAD readings and leaf N concentrations. Correlations between SPAD readings and leaf N were greater for the spring flush (r2 = 0.61) than for the summer flush (r2 = 0.49). High correlations of SPAD meter readings with either fruit yields or leaf N suggests that SPAD meter reading is an acceptable index of N status and fruit production of the trees. SPAD readings can be made rapidly without destructive sampling of the leaves. This study demonstrates that the SPAD meter can be used to evaluate the tree N status and improve a N fertilization program for grapefruit trees.

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 671c-671
Author(s):  
Y.C. Li ◽  
A.K. Alva ◽  
D.V. Calvert ◽  
M. Zhang

Rapid and accurate diagnosis of plant nutrient deficiency is critical for growers to use fertilizer economically and to minimize environmental concern. The objective of this study was to evaluate the use of leaf chlorophyll meter (SPAD-502) to predict nitrogen status and yield response of grapefruit trees. The study includes two irrigation treatments, three fertilizer sources, and four fertilizer rates. No significant differences in SPAD readings and leaf N were found between two irrigation treatments. Correlations between SPAD readings and leaf N were higher for the spring flush (r = 0.61) than that for summer flush (r = 0.49). There were poor relations between SPAD meter readings and the extractable chlorophyll content in the spring flush on a dry-weight basis (r = 0.25). Higher correlations between SPAD meter readings and N rates or fruit yields than between leaf N concentrations and N rates or fruit yield.


HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 508-512 ◽  
Author(s):  
Denise Neilsen ◽  
Eugene J. Hogue ◽  
Gerald H. Neilsen ◽  
Peter Parchomchuk

Four apple (Malus domestica Borkh) cultivars (`Fuji', `Spartan', `Fiesta', and `Gala') on Malling 9 (M.9) rootstock were grown in the field with three N rates (5, 20, and 35 g N/tree per year), supplied as Ca(NO3)2, and fertigated daily for 9 weeks. In the second year, leaf SPAD readings (chlorophyll readings obtained with the Minolta-502 SPAD meter) increased over the growing season for all cultivars, and leaf N decreased. Leaf SPAD and leaf N measurements increased in response to N fertigation rate at all sampling times. `Gala' consistently had lower SPAD readings than the other cultivars, and, with the exception of the first sampling time, `Fuji' had higher and `Fiesta' lower leaf N concentrations than other cultivars. There were strong relationships between leaf N concentration and SPAD readings for all cultivars until mid-July (r2 = 0.44 to 0.89), but not later in the growing season. Differences in SPAD readings and leaf N concentration due to cultivar and over time were as great as those due to N treatments, indicating that in the future, determination of critical SPAD values for apple leaves must be standardized for cultivar and sampling time. SPAD readings could be used to assess the need for N early in the growing season in fertigated orchards where rapid changes in nutrition programs can be undertaken readily.


Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 482
Author(s):  
Stanisław Kaniszewski ◽  
Artur Kowalski ◽  
Jacek Dysko ◽  
Giovanni Agati

The correct fertilization of vegetable crops is commonly determined on the basis of soil and plant costly destructive analyses, demanding more sustainable non-invasive optical detection. Here, we tested the ability of the combined transmittance/fluorescence leaf clip Dualex device for determining the nitrogen (N) status of cabbage plants. Fully developed leaves from plants grown under different N rates of 0; 100; 200; 300 kg N ha−1 in 2018 and 2019 were measured in the field by the Dualex sensor twice a year in July and October. The chlorophyll (Chl) and nitrogen (nitrogen balance index, NBI) indices and the flavonols (Flav) index of the sensor were positively and negatively correlated to leaf nitrogen, respectively. Merging the two-years data, the NBI versus leaf N correlation was less point dispersed in October than July (R2 = 0.76 and 0.64, respectively). NBI was also correlated to cabbage yield, better in July than October. Our results showed that the multiparametric Dualex device can be used as precision agriculture tool for the early prediction of plant N and cabbage yield with economic advantage for the growers and reduced environmental contamination due to nitrate leaching.


2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Jorge Atílio Benati ◽  
Gilberto Nava ◽  
Newton Alex Mayer

Abstract The objective of this research was to evaluate the technical feasibility of SPAD index in the diagnosis of N status in ‘Esmeralda’ peach. The field trial, with five N rates applied to the soil (0, 40, 80, 120 and 160 kg ha-1 of N as urea form) was conducted in a randomized block design with four replications. During the years 2014, 2015, 2016 and 2017, SPAD readings were carried out with a Minolta SPAD-502 chlorophyll meter, in the leaf middle part, in 40 leaves per plot. The leaf N content determination was performed in CHN-S Elemental Analyzer. SPAD index and leaf N content increased in a linear way in response to increasing N rates applied in soil. There was a positive linear correlation between SPAD index and N concentration in leaves (0.652; 0.756; 0.762 and 0.767, p<0.05). SPAD index can be used for nutritional N diagnosis purpose in peach when readings are taken between the 13th and 15th weeks after full bloom. In the range between 39 and 49, the SPAD index indicates the leaf N content is included in “normal” class (33 – 45 g kg-1) of agronomic interpretation for the States of Rio Grande do Sul and Santa Catarina, Brazil.


HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1497-1500 ◽  
Author(s):  
P.L. Minotti ◽  
D.E. Halseth ◽  
J.B. Sieczka

We report three N rate experiments conducted on a gravelly loam soil to assess the N status of potato (Solanum tuberosum L.) using a Minolta SPAD-502 chlorophyll meter. Highly significant linear and quadratic trends were obtained for the regression of N rate on marketable tuber yields and SPAD readings. SPAD readings were taken at four times during the growing season and decreased as plants aged. Based on regression analysis, the early season SPAD readings, associated with N rates giving maximum marketable tuber yields, ranged from 49 to 56 units depending on year, variety, and location. Potato variety significantly affected SPAD values in eight of the 12 situations where readings were obtained. Precision in interpretation was improved when the highest N rates were considered “reference strips” to standardize the SPAD readings across varieties and growing seasons. Our results suggest that field SPAD readings can readily identify severe N deficiency in potatoes, have the potential to identify situations where supplementary sidedressed N would not be necessary, but would be of limited value for identifying situations of marginal N deficiency unless reference strips are used.


HortScience ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Yun-wen Wang ◽  
Bruce L. Dunn ◽  
Daryl B. Arnall ◽  
Pei-sheng Mao

This research was conducted to investigate the potentials of normalized difference vegetation index (NDVI), a Soil-Plant Analyses Development (SPAD) chlorophyll meter, and leaf nitrogen (N) concentration [% dry matter (DM)] for rapid determination of N status in potted geraniums (Pelargonium ×hortorum). Two F1 cultivars were chosen to represent a dark-green leaf cultivar, Horizon Deep Red, and a light-green leaf cultivar, Horizon Tangerine, and were grown in a soilless culture system. All standard 6-inch (15.24-cm) pots filled with a medium received an initial top-dress application of 5 g controlled-release fertilizer (15N–9P–12K), then plants were supplemented with additional N in the form of urea at 0, 50, 100, or 200 mg·L−1 N every few days to produce plants ranging from N-deficient to N-sufficient. The NDVI readings of individual plants from a NDVI pocket sensor developed by Oklahoma State University were collected weekly until the flowering stage. Data on flower traits, including number of pedicels (NOP), number of full umbels per pot (NOFU), total number of flowers per pot (TNF), number of flowers per pedicel (NOF), and inflorescences diameter (IFD), were collected 3 months after initial fertilizer treatment. After measuring flower traits, pedicels were removed from each pot, and SPAD value, NDVI, and leaf N concentration (g·kg−1 DM) were measured simultaneously. Cultivar and N rate significantly affected all but two flower and one N status parameters studied. The coefficient of determination R2 showed that NOP, NOFU, and TNF traits were more related to the N rates and the status parameters studied for ‘Horizon Tangerine’ than for ‘Horizon Deep Red’. For the latter cultivar, NOP and TNF traits were highly related to NDVI and SPAD values than N rates and leaf N content parameters. Correlation analysis indicated that the NDVI readings (R2 = 0.848 and 0.917) and SPAD values (R2 = 0.861 and 0.950) were significantly related to leaf N content (g·kg−1 DM) between cultivars. However, sensitivity of the NDVI and chlorophyll values to N application rate in geranium was slightly less than leaf N content. Strong correlations (R2 = 0.974 and 0.979, respectively) between NDVI and SPAD values were found within cultivars. The results demonstrated NDVI and SPAD values can be used to estimate N status in geranium. Because the pocket NDVI sensor will be cheaper than the SPAD unit, it has an advantage in determining N content in potted ornamentals.


2006 ◽  
Vol 86 (4) ◽  
pp. 1037-1046 ◽  
Author(s):  
Yan Zhu ◽  
Yingxue Li ◽  
Wei Feng ◽  
Yongchao Tian ◽  
Xia Yao ◽  
...  

Non-destructive monitoring of leaf nitrogen (N) status can assist in growth diagnosis, N management and productivity forecast in field crops. The objectives of this study were to determine the relationships of leaf nitrogen concentration on a leaf dry weight basis (LNC) and leaf nitrogen accumulation per unit soil area (LNA) to ground-based canopy reflectance spectra, and to derive regression equations for monitoring N nutrition status in wheat (Triticum aestivum L.). Four field experiments were conducted with different N application rates and wheat cultivars across four growing seasons, and time-course measurements were taken on canopy spectral reflectance, LNC and leaf dry weights under the various treatments. In these studies, LNC and LNA in wheat increased with increasing N fertilization rates. The canopy reflectance differed significantly under varied N rates, and the pattern of response was consistent across the different cultivars and years. Overall, an integrated regression equation of LNC to normalized difference index (NDI) of 1220 and 710 nm of canopy reflectance spectra described the dynamic pattern of change in LNC in wheat. The ratios of several near infrared (NIR) bands to visible light were linearly related to LNA, with the ratio index (RI) of the average reflectance over 760, 810, 870, 950 and 1100 nm to 660 nm having the best index for quantitative estimation of LNA in wheat. When independent data were fit to the derived equations, the average root mean square error (RMSE) values for the predicted LNC and LNA relative to the observed values were no more than 15.1 and 15.2%, respectively, indicating a good fit. Our relationships of leaf N status to spectral indices of canopy reflectance can be potentially used for non-destructive and real-time monitoring of leaf N status in wheat. Key words: Wheat, leaf nitrogen concentration, leaf nitrogen accumulation, canopy reflectance, spectral index, nitrogen monitoring


2017 ◽  
pp. 161-166
Author(s):  
D. Hamilton ◽  
C. Martin ◽  
M. Bennet ◽  
M. Hearnden ◽  
C.A. Asis

2020 ◽  
Vol 12 (7) ◽  
pp. 1139
Author(s):  
Rui Dong ◽  
Yuxin Miao ◽  
Xinbing Wang ◽  
Zhichao Chen ◽  
Fei Yuan ◽  
...  

Nitrogen (N) is one of the most essential nutrients that can significantly affect crop grain yield and quality. The implementation of proximal and remote sensing technologies in precision agriculture has provided new opportunities for non-destructive and real-time diagnosis of crop N status and precision N management. Notably, leaf fluorescence sensors have shown high potential in the accurate estimation of plant N status. However, most studies using leaf fluorescence sensors have mainly focused on the estimation of leaf N concentration (LNC) rather than plant N concentration (PNC). The objectives of this study were to (1) determine the relationship of maize (Zea mays L.) LNC and PNC, (2) evaluate the main factors influencing the variations of leaf fluorescence sensor parameters, and (3) establish a general model to estimate PNC directly across growth stages. A leaf fluorescence sensor, Dualex 4, was used to test maize leaves with three different positions across four growth stages in two fields with different soil types, planting densities, and N application rates in Northeast China in 2016 and 2017. The results indicated that the total leaf N concentration (TLNC) and PNC had a strong correlation (R2 = 0.91 to 0.98) with the single leaf N concentration (SLNC). The TLNC and PNC were affected by maize growth stage and N application rate but not the soil type. When used in combination with the days after sowing (DAS) parameter, modified Dualex 4 indices showed strong relationships with TLNC and PNC across growth stages. Both modified chlorophyll concentration (mChl) and modified N balance index (mNBI) were reliable predictors of PNC. Good results could be achieved by using information obtained only from the newly fully expanded leaves before the tasseling stage (VT) and the leaves above panicle at the VT stage to estimate PNC. It is concluded that when used together with DAS, the leaf fluorescence sensor (Dualex 4) can be used to reliably estimate maize PNC across growth stages.


2015 ◽  
Vol 39 (4) ◽  
pp. 1127-1140 ◽  
Author(s):  
Eric Victor de Oliveira Ferreira ◽  
Roberto Ferreira Novais ◽  
Bruna Maximiano Médice ◽  
Nairam Félix de Barros ◽  
Ivo Ribeiro Silva

The use of leaf total nitrogen concentration as an indicator for nutritional diagnosis has some limitations. The objective of this study was to determine the reliability of total N concentration as an indicator of N status for eucalyptus clones, and to compare it with alternative indicators. A greenhouse experiment was carried out in a randomized complete block design in a 2 × 6 factorial arrangement with plantlets of two eucalyptus clones (140 days old) and six levels of N in the nutrient solution. In addition, a field experiment was carried out in a completely randomized design in a 2 × 2 × 2 × 3 factorial arrangement, consisting of two seasons, two regions, two young clones (approximately two years old), and three positions of crown leaf sampling. The field areas (regions) had contrasting soil physical and chemical properties, and their soil contents for total N, NH+4-N, and NO−3-N were determined in five soil layers, up to a depth of 1.0 m. We evaluated the following indicators of plant N status in roots and leaves: contents of total N, NH+4-N, NO−3-N, and chlorophyll; N/P ratio; and chlorophyll meter readings on the leaves. Ammonium (root) and NO−3-N (root and leaf) efficiently predicted N requirements for eucalyptus plantlets in the greenhouse. Similarly, leaf N/P, chlorophyll values, and chlorophyll meter readings provided good results in the greenhouse. However, leaf N/P did not reflect the soil N status, and the use of the chlorophyll meter could not be generalized for different genotypes. Leaf total N concentration is not an ideal indicator, but it and the chlorophyll levels best represent the soil N status for young eucalyptus clones under field conditions.


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