scholarly journals Using a Computer Spreadsheet and Compiler to Extend Growth Models to Greenhouse Growers

1993 ◽  
Vol 3 (2) ◽  
pp. 230-233
Author(s):  
George J. Wulster

A software application for the personal computer has been developed using the macro languages of Lotus l-2-3 Release 2.2 and the spreadsheet compiler Baler XE Release 1.0E to provide Easter lily (Lilium longiflorum Thunb.) growers with a tool to track and predict various developmental stages of the crop during greenhouse forcing.

2010 ◽  
Vol 135 (5) ◽  
pp. 445-455 ◽  
Author(s):  
Neil O. Anderson ◽  
Adnan Younis ◽  
Ye Sun

The large genome size of easter lily [Lilium longiflorum (77.1 pg/2C nucleus)], coupled with repetitive DNA sequences, makes it difficult to use molecular techniques to identify or fingerprint lily (Lilium) species, hybrids, and clones. Previous research demonstrated that amplified fragment length polymorphisms could not be optimized for consistency and repeatability to obtain reliable genetic variation assessments of lily species and clones. The objective of this research was to analyze the effectiveness and stringency of intersimple sequence repeats (ISSRs) to determine genetic differences between L. longiflorum ‘Nellie White’ clonal ramet populations from bulb growers over years. DNA from closely related clones of L. longiflorum ‘Nellie White’ included 2002 (n = 11 bulb lots) and 2003 (n = 12 bulb lots). Comparison cultivars and species were also included. Five University of British Columbia (UBC) primers (P808, P810, P811, P814, and P818) that were used produced 56 polymorphic loci. ISSR banding patterns were consistent among three replications within ‘Nellie White’ clonal genotypes. ‘Nellie White’ clones differed significantly within (82%) and among (18%) growers in 2002 and 2003. ‘Nellie White’ clones are not uniform or part of a single ramet population. Principal clades within years separated at Nei's genetic distances (GDs) of GD = 0.6 (growers 2, 4, and 12) to GD = 0.82 (grower 6) in 2002 and GD = 0.51 (grower 4) to GD = 0.78 (grower 14). The most closely related ‘Nellie White’ clones within growers ranged from GD = 0.8 to 0.95 in 2002 and GD = 0.7 to 0.91 for 2003. Five top-performing growers (1, and 3–6) from previous morphological studies and, particularly growers 3 and 5, were in similar clades, cosegregating with phenotypic traits of stem emergence and flowering dates. The lack of a meiotic sieve (Muller's ratchet) may be responsible for the high level of mutational differences present in the ‘Nellie White’ clones and significantly affects the ability of commercial greenhouse growers to produce a uniform easter lily crop, particularly in years when the Easter holiday is early.


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 776
Author(s):  
Shipra Kumari ◽  
Bashistha Kumar Kanth ◽  
Ju young Ahn ◽  
Jong Hwa Kim ◽  
Geung-Joo Lee

Genome-wide transcriptome analysis using RNA-Seq of Lilium longiflorum revealed valuable genes responding to biotic stresses. WRKY transcription factors are regulatory proteins playing essential roles in defense processes under environmental stresses, causing considerable losses in flower quality and production. Thirty-eight WRKY genes were identified from the transcriptomic profile from lily genotypes, exhibiting leaf blight caused by Botrytis elliptica. Lily WRKYs have a highly conserved motif, WRKYGQK, with a common variant, WRKYGKK. Phylogeny of LlWRKYs with homologous genes from other representative plant species classified them into three groups- I, II, and III consisting of seven, 22, and nine genes, respectively. Base on functional annotation, 22 LlWRKY genes were associated with biotic stress, nine with abiotic stress, and seven with others. Sixteen unique LlWRKY were studied to investigate responses to stress conditions using gene expression under biotic and abiotic stress treatments. Five genes—LlWRKY3, LlWRKY4, LlWRKY5, LlWRKY10, and LlWRKY12—were substantially upregulated, proving to be biotic stress-responsive genes in vivo and in vitro conditions. Moreover, the expression patterns of LlWRKY genes varied in response to drought, heat, cold, and different developmental stages or tissues. Overall, our study provides structural and molecular insights into LlWRKY genes for use in the genetic engineering in Lilium against Botrytis disease.


2000 ◽  
Vol 125 (2) ◽  
pp. 248-253 ◽  
Author(s):  
Anil P. Ranwala ◽  
William B. Miller ◽  
Terri I. Kirk ◽  
P. Allen Hammer

The interactions of ancymidol drenches, postgreenhouse cold storage, and hormone sprays on postharvest leaf chlorosis and flower longevity of `Nellie White' Easter lilies (Lilium longiflorum Thunb.) were investigated. Ancymidol drenches (0.5 mg/plant twice) during early growth resulted in leaf chlorosis in the greenhouse which intensified further during postharvest. Cold storage (4 °C) of puffy bud stage plants for 2 weeks also accelerated leaf chlorosis. The combination of ancymidol treatment with cold storage resulted in the most severe leaf chlorosis. Promalin (GA4+7 and BA each at 100 mg·L-1) sprays completely prevented postharvest leaf chlorosis, whereas ProGibb (GA3 at 1000 mg·L-1) was ineffective. Cold storage reduced flower longevity and increased bud abortion, however, the degree of bud abortion varied among experiments in different years. Both ProGibb and Promalin sprays increased flower longevity. Compared to positive DIF (difference between day and night temperature) grown plants, forcing under negative DIF (-8 °C) increased the severity of postharvest leaf chlorosis. Leaves were sampled from basal, middle, and upper sections of the stem after 4 and 12 days in a postharvest evaluation room, and analyzed for soluble carbohydrates and N. Total leaf soluble carbohydrates and N concentrations were less in basal and middle sections of negative DIF-grown plants than in positive DIF-grown plants. Leaf chlorosis was associated with depletion of soluble carbohydrates and N in the leaves. Chemical names used: α-cyclopropyl-α-(p-methoxyphenyl)-5-pyrimidinemethanol (ancymidol); gibberellic acid (GA3); gibberellins A4A7 (GA4+7); N-(phenylmethyl)-1H-purine 6-amine (benzyladenine).


2015 ◽  
Vol 63 (44) ◽  
pp. 9722-9728 ◽  
Author(s):  
Wenping Tang ◽  
John P. Munafo ◽  
Kimberly Palatini ◽  
Debora Esposito ◽  
Mou-Tuan Huang ◽  
...  

1997 ◽  
Vol 54 (1) ◽  
pp. 39-55 ◽  
Author(s):  
Paul R. Fisher ◽  
Royal D. Heins ◽  
Niels Ehler ◽  
J.Heinrich Lieth ◽  
Michael Brogaard ◽  
...  

1978 ◽  
Vol 58 (1) ◽  
pp. 287-290 ◽  
Author(s):  
M. J. TSUJITA ◽  
D. P. MURR ◽  
A. G. JOHNSON

Severity of lower foliage senescence of Easter lilies (Lilium longiflorum Thunb.) was increased in response to application of ancymidol (A-Rest) in combination with low phosphorus nutrition. Leaf senescence in lilies could be related to a reduced foliar content of phosphorus and total chlorophyll. Plant growth was significantly retarded by high rates of A-Rest. Maintaining high phosphorus nutrition lessened the severity of leaf senescence but partially overcame the effect of A-Rest on growth.


HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 189a-189
Author(s):  
Rosanne E. Franco ◽  
Susan S. Han

Senescence of lower leaves of Easter lilies (Lilium longiflorum Thunb.) was previously shown to be delayed with application of the growth regulators, gibberellic acid (GA3) and benzyladenine (BA). This study was done to determine the physiological effects of GA3 and BA in relation to the delay of leaf senescence. Foliar application with 500 ppm BA or GA3 delayed chlorosis and lowered respiration rate in Easter lily leaves. A combination of 500 ppm BA and 500 ppm GA3 was more effective than the individual application of each. Gibberellic acid, BA, or their combination before cold storage resulted in delayed chlorosis and lowered respiration following removal from cold storage. Treatment with growth regulators after cold storage was less effective. Senescence of leaves was not associated with ethylene since ethylene production by leaves was undetectable by gas chromatograph. In addition, pulsing or continuous treatment with silver thiosulphate (STS), an inhibitor of ethylene synthesis, did not delay foliar chlorosis. Analysis of carbohydrate levels in Easter lily leaves treated with GA3, BA, or their combination may contribute to the understanding of the physiological effects of these two growth regulators.


Sign in / Sign up

Export Citation Format

Share Document