Characterization of Contaminant Migration Potential Through In-Place Sediment Caps

2011 ◽  
Author(s):  
Philip Gidley ◽  
Seokjoon Kwon ◽  
Upal Ghosh
Keyword(s):  
Contaminant Migration ◽  
Migration Potential

Characterization of contaminant migration potential in the vicinity of an in-place sand cap

2010 ◽  
Vol 10 (3) ◽  
pp. 440-450 ◽  
Author(s):  
Karen A. Merritt ◽  
Ryan Fimmen ◽  
Bruce Sass ◽  
Eric Foote ◽  
Marc A. Mills ◽  
...  
Keyword(s):  
Contaminant Migration ◽  
Migration Potential ◽  
Sand Cap

Characterization of Contaminant Migration Potential Through In-Place Sediment Caps

2009 ◽  
Author(s):  
Bruce M. Sass ◽  
Ryan L. Fimmen ◽  
Eric A. Foote ◽  
Victor S. Magar ◽  
Upal Ghosh
Keyword(s):  
Contaminant Migration ◽  
Migration Potential

scholarly journals Characterization of a Stem-like Subpopulation in Basal-like Ductal Carcinoma in Situ (DCIS) Lesions

2013 ◽  
Vol 289 (3) ◽  
pp. 1303-1312 ◽  
Author(s):  
Qinglin Li ◽  
Gabriel Eades ◽  
Yuan Yao ◽  
Yongshu Zhang ◽  
Qun Zhou
Keyword(s):  
Cancer Cells ◽  
Aldehyde Dehydrogenase ◽  
Breast Cancer Cells ◽  
Ductal Carcinoma ◽  
Chemopreventive Agent ◽  
Aldehyde Dehydrogenase 1 ◽  
Aldh1 Expression ◽  
Migration Potential

Previously, we found that basal-like ductal carcinoma in situ (DCIS) contains cancer stem-like cells. Here, we characterize stem-like subpopulations in a model of basal-like DCIS and identify subpopulations of CD49f+/CD24− stem-like cells that possess aldehyde dehydrogenase 1 activity. We found that these cells show enhanced migration potential compared with non-stem DCIS cells. We also found that the chemopreventive agent sulforaphane can target these DCIS stem-like cells, reduce aldehyde dehydrogenase 1 (ALDH1) expression, and decrease mammosphere and progenitor colony formation. Furthermore, we characterized exosomal trafficking of microRNAs in DCIS and found that several microRNAs (miRs) including miR-140, miR-29a, and miR-21 are differentially expressed in exosomes from DCIS stem-like cells. We found that SFN treatment could reprogram DCIS stem-like cells as evidenced by significant changes in exosomal secretion more closely resembling that of non-stem cancer cells. Finally, we demonstrated that exosomal secretion of miR-140 might impact signaling in nearby breast cancer cells.

Phenotypic Characterization of Human CD34+ Cells Selected from G-CSF Mobilized Leukopheresis Using Baxter’s Isolex™ 300i Magnetic Cell Selection System (v2.5)

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4769-4769
Author(s):  
Amy Cohen ◽  
Delara Motlagh ◽  
David Amrani
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Selection ◽  
Selection System ◽  
Color Flow ◽  
Migratory Response ◽  
Cd34 Cells ◽  
Migration Potential ◽  
Colony Forming Assay

Abstract Baxter’s Isolex™ 300i Magnetic Cell Selection System (v2.5) is used to select CD34+ cells from G-CSF mobilized leukopheresis units. This study focused on evaluating the cellular composition of the post-Isolex product as well as producing a detailed characterization of the CD34+ cells. A multi-color flow cytometry assay was developed to characterize the Isolex product with a detailed focus on the CD34+ cells. A hematopoietic colony-forming assay was used to determine the clonal potential of the selected cells. Cell function was evaluated by measuring the migration potential of the CD34+ cells toward SDF-1. The post-Isolex cell suspension product contained an average of 96.44% ± 0.84% CD34+ cells. The majority of the remaining cells present were T and B cell lymphocytes with an average 1.74% ± 0.78% CD3 positive T-Cells and 1.61% ± 0.23% CD19 positive B-Cells. The platelets comprised 1.92% ± 0.92% of the product and 99% of this population were co-aggregated to the CD34+ cells. Non-CD34, immature white blood cells averaged less than 0.5% of the total post-Isolex population. A majority of the CD34+ stem cells are lineage-committed HPCs as determined by KDR-CD34+ (94.90% ± 1.99%) and CD38+CD34+ (93.87% ± 2.76%). Commitment to a myeloid cell line (CD33+CD34+) was determined to be 34.75% ± 23.10%. Migration potential of the stem cells toward SDF-1 as defined by dual positivity for CXCR4 and CD34 averaged 1.95% ± 2.49%. While the CXCR4 receptor positivity appears low, the migratory response of the CD34+ cells toward SDF-1 was robust. An examination of VEGF populations in the post-Isolex product averaged 0.32% ± 0.17% for VEGF R2 (KDR) and 34.25% ± 12.74% for VEGF R1. Several adhesion markers were assayed and the positivity was equal to or less than 1% for CD146, CD144, CD73, and CD29. Other adhesion markers, such as CD105 (endoglin) averaged 34.04% ± 20.64% and CD99 averaged 66.33% ± 13.25%. CD117, which binds to the receptor for stem cell factor (SCF), averaged 69.63% ± 6.38%. CD133, which is considered an early HPC marker, averaged 73.90% ± 5.97%. CD90, which is useful for defining a population of highly proliferative cells capable of long-term culture, averaged 60.50% ± 8.50%. The EPC phenotypic profile combination, KDR+CD34+CD133+, averaged 0.30% ± 0.22% in our post-Isolex population. In the hematopoietic colony-forming assay, the Isolex-selected CD34+ cells were able to produce colonies with an efficiency of 20% with CFU-GM forming a majority of the colonies. By utilizing multi-color flow cytometry, we were able to account for the cell populations found in the Isolex product and to further characterize the CD34+ selected cell population. The hematopoietic colony-forming assay gave insight into the efficiency and growth potential of the CD34+ selected cells. The functional migration assay determined that the CD34+ selected cells exhibited a robust migratory response toward SDF-1.

scholarly journals Separation and Characterization of Prostate Cancer Cell Subtype according to Their Motility Using a Multi-Layer CiGiP Culture

Micromachines ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 660 ◽  
Author(s):  
Lin-Xiang Wang ◽  
Ying Zhou ◽  
Jing-Jing Fu ◽  
Zhisong Lu ◽  
Ling Yu
Keyword(s):  
Prostate Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Middle Layer ◽  
Therapeutic Effects ◽  
Tumor Cell Migration ◽  
Cell Subtype ◽  
Migration Potential

Cancer cell metastasis has been recognized as one hallmark of malignant tumor progression; thus, measuring the motility of cells, especially tumor cell migration, is important for evaluating the therapeutic effects of anti-tumor drugs. Here, we used a paper-based cell migration platform to separate and isolate cells according to their distinct motility. A multi-layer cells-in-gels-in-paper (CiGiP) stack was assembled. Only a small portion of DU 145 prostate cancer cells seeded in the middle layer could successfully migrate into the top and bottom layers of the stack, showing heterogeneous motility. The cells with distinct migration were isolated for further analysis. Quantitative PCR assay results demonstrated that cells with higher migration potential had increased expression of the ALDH1A1, SRY (sex-determining region Y)-box 2, NANOG, and octamer-binding transcription 4. Increased doxorubicin tolerance was also observed in cells that migrated through the CiGiP layers. In summary, the separation and characterization of prostate cancer cell subtype can be achieved by using the multi-layer CiGiP cell migration platform.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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