Nf-Kappab as a Critical Biological Link Between Psychological Stress and Breast Cancer

2007 ◽  
Author(s):  
Fiona Yull
Keyword(s):  
Breast Cancer ◽  
Psychological Stress ◽  
Nf Kappab

scholarly journals Co-relation with novel phosphorylation sites of IκBα and necroptosis in breast cancer cells

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sung Hoon Choi ◽  
Hee-Sub Yoon ◽  
Shin-Ae Yoo ◽  
Sung Ho Yun ◽  
Joo-Hee Park ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Phosphorylation Site ◽  
Aurora Kinase ◽  
Serine Phosphorylation ◽  
Flight Mass Spectrometry ◽  
Iκb Kinase ◽  
Orbitrap Mass Spectrometer ◽  
Nf Kappab

Abstract Background Phosphorylation of NF-kappaB inhibitor alpha (IκBα) is key to regulation of NF-κB transcription factor activity in the cell. Several sites of IκBα phosphorylation by members of the IκB kinase family have been identified, but phosphorylation of the protein by other kinases remains poorly understood. We investigated a new phosphorylation site on IκBα and identified its biological function in breast cancer cells. Methods Previously, we observed that aurora kinase (AURK) binds IκBα in the cell. To identify the domains of IκBα essential for phosphorylation by AURK, we performed kinase assays with a series of IκBα truncation mutants. AURK significantly promoted activation of IκBα at serine 32 but not serine 36; by contrast, IκB kinase (IKK) family proteins activated both of these residues. We also confirmed phosphorylation of IκBα by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and nano-liquid chromatography hybrid quadrupole orbitrap mass spectrometer (nanoLC-MS/MS; Q-Exactive). Results We identified two novel sites of serine phosphorylation, S63 and S262. Alanine substitution of S63 and S262 (S63A and S262A) of IκBα inhibited proliferation and suppressed p65 transcription activity. In addition, S63A and/or S262A of IκBα regulated apoptotic and necroptotic effects in breast cancer cells. Conclusions Phosphorylation of IκBα by AURK at novel sites is related to the apoptosis and necroptosis pathways in breast cancer cells.

scholarly journals TNFalpha up-regulates SLUG via the NF-kappaB/HIF1alpha axis, which imparts breast cancer cells with a stem cell-like phenotype

2010 ◽  
Vol 225 (3) ◽  
pp. 682-691 ◽  
Author(s):  
Gianluca Storci ◽  
Pasquale Sansone ◽  
Sara Mari ◽  
Gabriele D'Uva ◽  
Simona Tavolari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Nf Kappab

scholarly journals NF-kappaB Regulates Redox Status in Breast Cancer Subtypes

Genes ◽  
2018 ◽  
Vol 9 (7) ◽  
pp. 320 ◽  
Author(s):  
Bruno Pires ◽  
Renata Binato ◽  
Gerson Ferreira ◽  
Rubens Cecchini ◽  
Carolina Panis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Redox Status ◽  
Breast Cancer Subtypes ◽  
Cancer Subtypes ◽  
Nf Kappab

Stronger endocrine responses after brief psychological stress in women at familial risk of breast cancer

2003 ◽  
Vol 28 (4) ◽  
pp. 584-593 ◽  
Author(s):  
S.M. Gold ◽  
S.G. Zakowski ◽  
H.B. Valdimarsdottir ◽  
D.H. Bovbjerg
Keyword(s):  
Breast Cancer ◽  
Psychological Stress ◽  
Familial Risk
Sign in / Sign up

Export Citation Format

Share Document