Radiation Biomarker Research Using Mass Spectrometry

2007 ◽  
Author(s):  
Stephan B. Bach ◽  
Walter Hubert
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Research

scholarly journals Analytical Considerations of Stable Isotope Labelling in Lipidomics

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 151 ◽  
Author(s):  
Alexander Triebl ◽  
Markus Wenk
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Clinical Settings ◽  
Stable Isotope Labelling ◽  
Stable Isotope Label ◽  
Isotope Labelling ◽  
Isotope Label ◽  
Lipid Biomarker ◽  
Staple Technique ◽  
Biomarker Research

Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in clinical settings. Stable isotope labelling has become a staple technique in the analysis of small molecule metabolism and dynamics, as it is the only experimental setup by which biosynthesis, remodelling and degradation of biomolecules can be directly measured. Using state-of-the-art analytical technologies such as chromatography-coupled high resolution tandem mass spectrometry, the stable isotope label can be precisely localized and quantified within the biomolecules. The application of stable isotope labelling to lipidomics is however complicated by the diversity of lipids and the complexity of the necessary data analysis. This article discusses key experimental aspects of stable isotope labelling in the field of mass spectrometry-based lipidomics, summarizes current applications and provides an outlook on future developments and potential.

scholarly journals Mass spectrometry in cancer biomarker research: a case for immunodepletion of abundant blood-derived proteins from clinical tissue specimens

2014 ◽  
Vol 8 (2) ◽  
pp. 269-286 ◽  
Author(s):  
DaRue A Prieto ◽  
Donald J Johann ◽  
Bih-Rong Wei ◽  
Xiaoying Ye ◽  
King C Chan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Biomarker ◽  
Tissue Specimens ◽  
Biomarker Research

Catch and measure–mass spectrometry‐based immunoassays in biomarker research

2014 ◽  
Vol 1844 (5) ◽  
pp. 927-932 ◽  
Author(s):  
Frederik Weiß ◽  
Bart H.J. van den Berg ◽  
Hannes Planatscher ◽  
Christopher J. Pynn ◽  
Thomas O. Joos ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Research

scholarly journals A dry method for preserving tear protein samples

2017 ◽  
Author(s):  
Weiwei Qin ◽  
Chan Zhao ◽  
Linpei Zhang ◽  
Ting Wang ◽  
Youhe Gao
Keyword(s):  
Mass Spectrometry ◽  
Large Scale ◽  
Low Cost ◽  
Mass Spectrometry Analysis ◽  
Protein Patterns ◽  
Spectrometry Analysis ◽  
Disease Biomarkers ◽  
Tear Protein ◽  
Significant Difference ◽  
Biomarker Research

AbstractTears covering the ocular surface is an important bio-fluid containing thousands of molecules, including proteins, lipids, metabolites, nucleic acids, and electrolytes. Tears are valuable resources for biomarker research of ocular and even systemic diseases. For application in biomarker studies, tear samples should ideally be stored using a simple, low-cost, and efficient method along with the patient’s medical records. For this purpose, we developed a novel Schirmer’s strip-based dry method that allows for storage of tear samples in vacuum bags at room temperature. Using this method, tear protein patterns can also be preserved. Liquid chromatography-mass spectrometry/mass spectrometry analysis of proteins recovered from the dry method and traditional wet method showed no significant difference. Some tissue/organ enriched proteins were identified in tear, thus tear might be a good window for monitoring the change of these tissues or organs. This dry method facilitates sample transportation and enables the storage of tear samples on a large scale, increasing the availability of samples for studying disease biomarkers in tears.

Sign in / Sign up

Export Citation Format

Share Document