The Functions of BRCA2 in Homologous Recombinational Repair

2006 ◽  
Author(s):  
David J. Chen
Keyword(s):  
Recombinational Repair ◽  
Homologous Recombinational Repair

Human SIRT2 and SIRT3 deacetylases function in DNA homologous recombinational repair

2021 ◽  
Author(s):  
Takeshi Yasuda ◽  
Kazuya Takizawa ◽  
Ayako Ui ◽  
Michio Hama ◽  
Wataru Kagawa ◽  
...  
Keyword(s):  
Recombinational Repair ◽  
Homologous Recombinational Repair

scholarly journals Regulation of homologous recombinational repair by lamin B1 in radiation‐induced DNA damage

2015 ◽  
Vol 29 (6) ◽  
pp. 2514-2525 ◽  
Author(s):  
Ning‐Ang Liu ◽  
Jiying Sun ◽  
Kazuteru Kono ◽  
Yasunori Horikoshi ◽  
Tsuyoshi Ikura ◽  
...  
Keyword(s):  
Dna Damage ◽  
Recombinational Repair ◽  
Lamin B1 ◽  
Homologous Recombinational Repair ◽  
Radiation Induced

scholarly journals In Vitro Evidence for Homologous Recombinational Repair in Resistance to Melphalan

2001 ◽  
Vol 93 (19) ◽  
pp. 1473-1478 ◽  
Author(s):  
Z.-M. Wang ◽  
Z.-P. Chen ◽  
Z.-Y. Xu ◽  
G. Christodoulopoulos ◽  
V. Bello ◽  
...  
Keyword(s):  
Recombinational Repair ◽  
Homologous Recombinational Repair

scholarly journals Roles of Vertebrate Smc5 in Sister Chromatid Cohesion and Homologous Recombinational Repair

2011 ◽  
Vol 31 (7) ◽  
pp. 1369-1381 ◽  
Author(s):  
A. K. Stephan ◽  
M. Kliszczak ◽  
H. Dodson ◽  
C. Cooley ◽  
C. G. Morrison
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Cohesion ◽  
Recombinational Repair ◽  
Homologous Recombinational Repair ◽  
Chromatid Cohesion

scholarly journals Homologous Recombinational Repair Factors Are Recruited and Loaded onto the Viral DNA Genome in Epstein-Barr Virus Replication Compartments

2009 ◽  
Vol 83 (13) ◽  
pp. 6641-6651 ◽  
Author(s):  
Ayumi Kudoh ◽  
Satoko Iwahori ◽  
Yoshitaka Sato ◽  
Sanae Nakayama ◽  
Hiroki Isomura ◽  
...  
Keyword(s):  
Dna Replication ◽  
Homologous Recombination ◽  
Viral Genome ◽  
Epstein Barr Virus ◽  
Lytic Replication ◽  
Recombinational Repair ◽  
Viral Dna ◽  
Barr Virus ◽  
Homologous Recombinational Repair ◽  
Epstein Barr

ABSTRACT Homologous recombination is an important biological process that facilitates genome rearrangement and repair of DNA double-strand breaks (DSBs). The induction of Epstein-Barr virus (EBV) lytic replication induces ataxia telangiectasia-mutated (ATM)-dependent DNA damage checkpoint signaling, leading to the clustering of phosphorylated ATM and Mre11/Rad50/Nbs1 (MRN) complexes to sites of viral genome synthesis in nuclei. Here we report that homologous recombinational repair (HRR) factors such as replication protein A (RPA), Rad51, and Rad52 as well as MRN complexes are recruited and loaded onto the newly synthesized viral genome in replication compartments. The 32-kDa subunit of RPA is extensively phosphorylated at sites in accordance with those with ATM. The hyperphosphorylation of RPA32 causes a change in RPA conformation, resulting in a switch from the catalysis of DNA replication to the participation in DNA repair. The levels of Rad51 and phosphorylated RPA were found to increase with the progression of viral productive replication, while that of Rad52 proved constant. Furthermore, biochemical fractionation revealed increases in levels of DNA-bound forms of these HRRs. Bromodeoxyuridine-labeled chromatin immunoprecipitation and PCR analyses confirmed the loading of RPA, Rad 51, Rad52, and Mre11 onto newly synthesized viral DNA, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling analysis demonstrated DSBs in the EBV replication compartments. HRR factors might be recruited to repair DSBs on the viral genome in viral replication compartments. RNA interference knockdown of RPA32 and Rad51 prevented viral DNA synthesis remarkably, suggesting that homologous recombination and/or repair of viral DNA genome might occur, coupled with DNA replication to facilitate viral genome synthesis.

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