Low Melatonin Production During Adulthood - Phase 2: Association with Levels of Hydroxyl Radical Scavenging and DNA Damage

2005 ◽  
Author(s):  
Eugene Sobel ◽  
Zoreh Davanipour
Keyword(s):  
Dna Damage ◽  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Phase 2 ◽  
Melatonin Production

Effect of Hydroxyl Radical Scavenging Capacity on Clustering of DNA Damage

1997 ◽  
Vol 148 (4) ◽  
pp. 325 ◽  
Author(s):  
J. R. Milligan ◽  
J. A. Aguilera ◽  
C. C. Wu ◽  
R. A. Paglinawan ◽  
T-T. D. Nguyen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation

2011 ◽  
Vol 49 (9) ◽  
pp. 2439-2444 ◽  
Author(s):  
Wei Chen ◽  
Yudong Li ◽  
Jianrong Li ◽  
Qiang Han ◽  
Libin Ye ◽  
...  
Keyword(s):  
Dna Damage ◽  
Hydroxyl Radical ◽  
Radical Formation

8. Evaluation of Snow Fungus Polysaccharides on benzo[a]pyrene-induced DNA Damage

2018 ◽  
Author(s):  
Daisy Liu
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Free Radical ◽  
Radical Scavenging ◽  
Chinese Hamster ◽  
Negative Control ◽  
Lung Fibroblasts ◽  
Protective Effects ◽  
Tremella Fuciformis

Snow fungus, Tremella fuciformis, has been demonstrated to have numerous health benefits including purported chemopreventive properties due to free radical-scavenging ability. Protective effects derived from snow fungus polysaccharides are evaluated on Chinese hamster lung fibroblasts (CCL-39) exposed to carcinogen benzo[a]pyrene known to cause free radical formation and oxidative stress to cells. In this experiment, it was hypothesized that the naturally occurring polysaccharides in snow fungus are able to protect against or reduce oxidative stress-induced DNA damage. Polysaccharides were isolated through an alkaline extraction and in-vitro digestion. DNA damage was measured using the single-cell gel electrophoresis comet assay after exposure to benzo[a]pyrene and polysaccharide extract to lung fibroblasts. Results were calculated using the mean and standard deviation data of tail length and area, respectively. Each damaged cell was measured and analyzed through ImageJ Editing Software. The results indicate a promising trend which depict snow fungus polysaccharides yielding lower levels of DNA damage compared to cells exposed to benzo[a]pyrene and compared to the negative control (phosphate buffered saline and Dulbecco’s cell medium). This study suggests polysaccharides from Tremella fuciformis could truly prevent cellular DNA damage by protecting against oxidative stress.

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