Specific Inhibition of HER-2/Neu Transcription Initiation

2005 ◽  
Author(s):  
Scot W. Ebbinghaus
Keyword(s):  
Transcription Initiation ◽  
Specific Inhibition ◽  
Her 2

scholarly journals Label-free Detection of the Transcription Initiation Factor Assembly and Specific Inhibition by Aptamers

2014 ◽  
Vol 35 (5) ◽  
pp. 1279-1284 ◽  
Author(s):  
Shuo Ren ◽  
Yuanyuan Jiang ◽  
Hye Rim Yoon ◽  
Sun Woo Hong ◽  
Donghyuk Shin ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Initiation Factor ◽  
Specific Inhibition ◽  
Label Free ◽  
Transcription Initiation Factor ◽  
Label Free Detection

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

High resolution mapping of nucleic acid containing complexes in vitro and in situ

1996 ◽  
Vol 54 ◽  
pp. 48-49
Author(s):  
D. P. Bazett-Jones ◽  
M. J. Hendzel
Keyword(s):  
Nucleic Acid ◽  
High Resolution ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Heavy Atom ◽  
Regulation Of Transcription ◽  
High Exposure ◽  
Resolution Mapping ◽  
Tata Sequence

Structural analysis of combinations of nucleosomes and transcription factors on promoter and enhancer elements is necessary in order to understand the molecular mechanisms responsible for the regulation of transcription initiation. Such complexes are often not amenable to study by high resolution crystallographic techniques. We have been applying electron spectroscopic imaging (ESI) to specific problems in molecular biology related to transcription regulation. There are several advantages that this technique offers in studies of nucleoprotein complexes. First, an intermediate level of spatial resolution can be achieved because heavy atom contrast agents are not necessary. Second, mass and stoichiometric relationships of protein and nucleic acid can be estimated by phosphorus detection, an element in much higher proportions in nucleic acid than protein. Third, wrapping or bending of the DNA by the protein constituents can be observed by phosphorus mapping of the complexes. Even when ESI is used with high exposure of electrons to the specimen, important macromolecular information may be provided. For example, an image of the TATA binding protein (TBP) bound to DNA is shown in the Figure (top panel). It can be seen that the protein distorts the DNA away from itself and much of its mass sits off the DNA helix axis. Moreover, phosphorus and mass estimates demonstrate whether one or two TBP molecules interact with this particular promoter TATA sequence.

432: The Genetic Polymorphisms of HPC/ELAC2 and HER-2 Gene Associate with Japanese Sporadic Prostate Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 114-114
Author(s):  
Akira Yokomizo ◽  
Hirofumi Koga ◽  
Koji Okumura ◽  
Tatsuo Konomoto ◽  
Taiji Tsukamoto ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Genetic Polymorphisms ◽  
Her 2

Jab1 is overexpressed in human breast cancer and is a downstream target for HER-2/neu

2008 ◽  
Author(s):  
Ming-Chuan Hsu ◽  
Chee-Yin Chai ◽  
Ming-Feng Hou ◽  
Hui-Chiu Chang ◽  
Wan-Tzu Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Downstream Target ◽  
Her 2

Die Bedeutung des stabilisierenden Effekts von Lapatinib auf HER-2 an der Membranoberfläche von Mamma- bzw. Ovarialkarzinom-Linien

2009 ◽  
Vol 69 (03) ◽  
Author(s):  
V Porto ◽  
I Mutz-Dehbalaie ◽  
J Rössler ◽  
A Wiedemair ◽  
A Sapinsky ◽  
...  
Keyword(s):  
Her 2

HER-2 gene amplification in pancreatic cancer

2004 ◽  
Vol 42 (05) ◽  
Author(s):  
K Borka ◽  
A Kiss ◽  
A Tőkés ◽  
P Kaliszky ◽  
P Kupcsulik ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Gene Amplification ◽  
Her 2
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