High Throughput Synthesis and Screening for Agents Inhibiting Androgen Receptor Mediated Gene Transcription

2004 ◽  
Author(s):  
Dale L. Boger
Keyword(s):  
Androgen Receptor ◽  
Gene Transcription ◽  
High Throughput

Abstract 4805: A quantitative high-throughput screen identifies novel potential epigenetic modulators of gene transcription

2010 ◽  
Author(s):  
Jennifer L. Wichterman ◽  
Ruili Huang ◽  
Wenwei Huang ◽  
Christopher Austin ◽  
James Inglese ◽  
...  
Keyword(s):  
Gene Transcription ◽  
High Throughput ◽  
High Throughput Screen

scholarly journals A High-Throughput Ligand Competition Binding Assay for the Androgen Receptor and Other Nuclear Receptors

2008 ◽  
Vol 14 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Clémentine Féau ◽  
Leggy A. Arnold ◽  
Aaron Kosinski ◽  
R. Kiplin Guy
Keyword(s):  
Androgen Receptor ◽  
Nuclear Receptors ◽  
High Throughput ◽  
High Throughput Screening ◽  
Low Cost ◽  
Environmental Chemicals ◽  
Binding Assays ◽  
Receptor Ligands ◽  
Competition Binding ◽  
Nuclear Receptor Ligands

Standardized, automated ligand-binding assays facilitate evaluation of endocrine activities of environmental chemicals and identification of antagonists of nuclear receptor ligands. Many current assays rely on fluorescently labeled ligands that are significantly different from the native ligands. The authors describe a radiolabeled ligand competition scintillation proximity assay (SPA) for the androgen receptor (AR) using Ni-coated 384-well FlashPlates® and liganded AR-LBD protein. This highly reproducible, low-cost assay is well suited for automated high-throughput screening. In addition, the authors show that this assay can be adapted to measure ligand affinities for other nuclear receptors (peroxisome proliferation-activated receptor γ, thyroid receptors α and β). ( Journal of Biomolecular Screening 2009:43-48)

scholarly journals A multifunctional androgen receptor screening assay using the high-throughput Hypercyt®flow cytometry system

2008 ◽  
Vol 73A (5) ◽  
pp. 390-399 ◽  
Author(s):  
Megan K. Dennis ◽  
Harmony J.C. Bowles ◽  
Debra A. MacKenzie ◽  
Scott W. Burchiel ◽  
Bruce S. Edwards ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Androgen Receptor ◽  
High Throughput ◽  
Screening Assay

scholarly journals Determining direct binders of the Androgen Receptor using a high-throughput Cellular Thermal Shift Assay

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Joseph Shaw ◽  
Mathew Leveridge ◽  
Charlotta Norling ◽  
Jakob Karén ◽  
Daniel Martinez Molina ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
High Throughput ◽  
Thermal Shift Assay ◽  
Cellular Thermal Shift Assay ◽  
Thermal Shift
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