Development of a Novel Tissue Slice Culture Model of Human Prostate Cancer

2003 ◽  
Author(s):  
Donna M. Peehl
Keyword(s):  
Prostate Cancer ◽  
Tissue Slice ◽  
Human Prostate ◽  
Slice Culture ◽  
Human Prostate Cancer ◽  
Culture Model

scholarly journals Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics

2017 ◽  
Vol 56 (31) ◽  
pp. 8992-8997 ◽  
Author(s):  
David R. Spiciarich ◽  
Rosalie Nolley ◽  
Sophia L. Maund ◽  
Sean C. Purcell ◽  
Jason Herschel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tissue Slice ◽  
Human Prostate ◽  
Cancer Tissue ◽  
Human Prostate Cancer ◽  
Bioorthogonal Labeling ◽  
Prostate Cancer Tissue

scholarly journals Androgen-regulated Formation and Degradation of Gap Junctions in Androgen-responsive Human Prostate Cancer Cells

2006 ◽  
Vol 17 (12) ◽  
pp. 5400-5416 ◽  
Author(s):  
Shalini Mitra ◽  
Lakshmanan Annamalai ◽  
Souvik Chakraborty ◽  
Kristen Johnson ◽  
Xiao-Hong Song ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gap Junctions ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Junctional Communication ◽  
Culture Model ◽  
Associated Proteins ◽  
A Cell

The constituent proteins of gap junctions, called connexins (Cxs), have a short half-life. Despite this, the physiological stimuli that control the assembly of Cxs into gap junctions and their degradation have remained poorly understood. We show here that in androgen-responsive human prostate cancer cells, androgens control the expression level of Cx32—and hence the extent of gap junction formation—post-translationally. In the absence of androgens, a major fraction of Cx32 is degraded presumably by endoplasmic reticulum–associated degradation, whereas in their presence, this fraction is rescued from degradation. We also show that Cx32 and Cx43 degrade by a similar mechanism. Thus, androgens regulate the formation and degradation of gap junctions by rerouting the pool of Cxs, which normally would have been degraded from the early secretory compartment, to the cell surface, and enhancing assembly into gap junctions. Androgens had no significant effect on the formation and degradation of adherens and tight junction–associated proteins. The findings that in a cell culture model that mimics the progression of human prostate cancer, degradation of Cxs, as well as formation of gap junctions, are androgen-dependent strongly implicate an important role of junctional communication in the prostate morphogenesis and oncogenesis.

Abstract PR2: Optimization and applications of a tissue slice culture model of the normal and malignant human prostate

2012 ◽  
Vol 72 (4 Supplement) ◽  
pp. PR2-PR2
Author(s):  
Sophia Lisette Maund ◽  
Rosalie Nolley ◽  
Donna Peehl
Keyword(s):  
Tissue Slice ◽  
Human Prostate ◽  
Slice Culture ◽  
Culture Model

A novel tissue-slice culture model for non-malignant human prostate

2008 ◽  
Vol 332 (3) ◽  
pp. 489-498 ◽  
Author(s):  
M. Bläuer ◽  
T. L. Tammela ◽  
T. Ylikomi
Keyword(s):  
Tissue Slice ◽  
Human Prostate ◽  
Slice Culture ◽  
Culture Model

scholarly journals Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics

2017 ◽  
Vol 129 (31) ◽  
pp. 9120-9125 ◽  
Author(s):  
David R. Spiciarich ◽  
Rosalie Nolley ◽  
Sophia L. Maund ◽  
Sean C. Purcell ◽  
Jason Herschel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tissue Slice ◽  
Human Prostate ◽  
Cancer Tissue ◽  
Human Prostate Cancer ◽  
Bioorthogonal Labeling ◽  
Prostate Cancer Tissue

scholarly journals Metabolic Reprogramming and Validation of Hyperpolarized13C Lactate as a Prostate Cancer Biomarker Using a Human Prostate Tissue Slice Culture Bioreactor

The Prostate ◽  
2013 ◽  
Vol 73 (11) ◽  
pp. 1171-1181 ◽  
Author(s):  
Kayvan R. Keshari ◽  
Renuka Sriram ◽  
Mark Van Criekinge ◽  
David M. Wilson ◽  
Zhen J. Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Biomarker ◽  
Tissue Slice ◽  
Metabolic Reprogramming ◽  
Prostate Tissue ◽  
Human Prostate ◽  
Slice Culture ◽  
Human Prostate Tissue

629: Novel Laser Activated Gold Nanoshell Thermal Ablation of Human Prostate Cancer in Vivo

2007 ◽  
Vol 177 (4S) ◽  
pp. 210-211 ◽  
Author(s):  
Joshua M. Stern ◽  
Jennifer Stanfield ◽  
Jer-Tsang Hsieh ◽  
Jeffrey A. Cadeddu
Keyword(s):  
Prostate Cancer ◽  
Thermal Ablation ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Gold Nanoshell
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