scholarly journals Genome-Wide Nucleic Acid/Protein Interactions in Breast Cancer

2003 ◽  
Author(s):  
Pamela A. Silver
Keyword(s):  
Breast Cancer ◽  
Nucleic Acid ◽  
Protein Interactions ◽  
Genome Wide ◽  
Acid Protein

Detection of nucleic acid–protein interactions in plant leaves using fluorescence lifetime imaging microscopy

2017 ◽  
Vol 12 (9) ◽  
pp. 1933-1950 ◽  
Author(s):  
Laurent Camborde ◽  
Alain Jauneau ◽  
Christian Brière ◽  
Laurent Deslandes ◽  
Bernard Dumas ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Lifetime ◽  
Protein Interactions ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Plant Leaves ◽  
Lifetime Imaging ◽  
Acid Protein

Fluorometric assays in the study of nucleic acid-protein interactions

1978 ◽  
Vol 90 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Angel Pestaña ◽  
Ricardo Castro ◽  
JoséV. Castell ◽  
Roberto Marco
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Acid Protein

Nucleic acid–protein interactions: Wedding for love or circumstances?

Biochimie ◽  
2009 ◽  
Vol 91 (8) ◽  
pp. 943-950
Author(s):  
Christophe Lavelle ◽  
Malcolm Buckle
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Acid Protein

Fluorometric assays in the study of nucleic acid-protein interactions

1978 ◽  
Vol 90 (2) ◽  
pp. 551-560 ◽  
Author(s):  
JoséV. Castell ◽  
Angel Pestaña ◽  
Ricardo Castro ◽  
Roberto Marco
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Acid Protein

scholarly journals Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins

2018 ◽  
Vol 1 (3) ◽  
pp. e201800088 ◽  
Author(s):  
Claudio Asencio ◽  
Aindrila Chatterjee ◽  
Matthias W Hentze
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Solid Phase ◽  
Protein Complexes ◽  
Simple Procedure ◽  
Silica Matrix ◽  
Cellular Functions ◽  
Dna And Rna ◽  
Acid Protein ◽  
Huh7 Cells

Proteins interact with nucleic acids to regulate cellular functions. The study of these regulatory interactions is often hampered by the limited efficiency of current protocols to isolate the relevant nucleic acid–protein complexes. In this report, we describe a rapid and simple procedure to highly enrich cross-linked nucleic acid–bound proteins, referred to as “2C” for “complex capture.” This method is based on the observation that silica matrix–based columns used for nucleic acid purification also effectively retain UV cross-linked nucleic acid–protein complexes. As a proof of principle, 2C was used to isolate RNA-bound proteins from yeast and mammalian Huh7 cells. The 2C method makes RNA labelling redundant, and specific RNA–protein interactions can be observed and validated by Western blotting. RNA–protein complexes isolated by 2C can subsequently be immunoprecipitated, showing that 2C is in principle compatible with sensitive downstream applications. We suggest that 2C can dramatically simplify the study of nucleic acid–protein interactions and benefit researchers in the fields of DNA and RNA biology.

In search of the nature of specific nucleic acid-protein interactions

2005 ◽  
Vol 92 (1) ◽  
pp. 1-10 ◽  
Author(s):  
JC Biro ◽  
Z Benyó ◽  
C Sansom ◽  
B Benyó
Keyword(s):  
Nucleic Acid ◽  
Protein Interactions ◽  
Acid Protein
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